Biological three-dimensional printing preparation method of chondrocyte loading anti-inflammatory meniscus bracket

A chondrocyte and three-dimensional printing technology, applied in tissue regeneration, medical science, prosthesis, etc., to avoid the operation of in vitro cell culture and promote regeneration

Inactive Publication Date: 2018-08-17
SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, there are no reports or applications of meniscal scaffolds that can have anti-inflammatory effects

Method used

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  • Biological three-dimensional printing preparation method of chondrocyte loading anti-inflammatory meniscus bracket
  • Biological three-dimensional printing preparation method of chondrocyte loading anti-inflammatory meniscus bracket
  • Biological three-dimensional printing preparation method of chondrocyte loading anti-inflammatory meniscus bracket

Examples

Experimental program
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Effect test

preparation example Construction

[0044] A method for preparing three-dimensional bioprinting of an anti-inflammatory meniscus stent loaded with chondrocytes. The method for preparing three-dimensional bioprinting of an anti-inflammatory meniscus stent loaded with chondrocytes includes the following steps:

[0045] S1. Construct a three-dimensional model of the meniscus:

[0046] 1. Data scanning: First, scan the knee joint on the healthy side of the patient with a three-dimensional scanning electron microscope, and then store the raw data obtained from the scan in the database;

[0047] 2. Data processing: segment and edit the knee joint model of the patient’s healthy side saved in step one through the bio-three-dimensional printer software to obtain a three-dimensional model of the outer or inner side of the knee joint meniscus. The three-dimensional model is mirrored to obtain a three-dimensional model of the meniscus suitable for the defect side of the patient, and then the three-dimensional meniscus model obtain...

Embodiment 1

[0073] 1. Perform nano-level 3D scanning electron microscopy on the left healthy knee joint of the patient, scan the original data, and store it in the database; segment and edit the knee joint image in the biological 3D printer software to obtain the outer side of the meniscus between the left knee joint Three-dimensional model. It is mirrored to obtain a three-dimensional model suitable for the outer side of the patient's right meniscus. Import the 3D data into the biological 3D printer software, perform partial correction and curved surface processing in turn, and save it as a stl format file.

[0074] 2. The processed three-dimensional meniscus model is sliced ​​and layered, and the printed polycaprolactone material and the hydrogel material loaded with fullerene and chondrocytes are respectively set into two printing paths. The two printing paths intersect layer by layer.

[0075] 3. After mixing the collagen and sodium alginate, add them to the DMEM medium, stir to dissolve...

Embodiment 2

[0079] The chondrocyte-loaded anti-inflammatory meniscus scaffold prepared by using the three-dimensional bioprinting technology in Example 1 above was used to characterize the cell survival rate. The scaffold was placed in a 12-well plate for culture, and then the chondrocyte viability in the scaffold was detected by the MTT method. After culturing for 1, 3, 5, 7 and 9 days, discard the old medium and add PBS to rinse to remove dead cells. Add 500 μL of serum-free medium and 1500 μL of MTT solution to each well and incubate at 37°C for 4 hours. Then add 500μL of DMSO to each well to fully dissolve the formazan crystals, take 100μL of the dissolving solution and use a microplate reader to detect the absorbance at a wavelength of 492nm. Three parallel hole experiments were performed at each time point.

[0080] MTT results like image 3 It is shown that the chondrocytes on the scaffold have good proliferation behavior and their cell viability is good.

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Abstract

The invention relates to the field of biological three-dimensional printed meniscus bracket, in particular to a biological three-dimensional printing preparation method of a chondrocyte loading anti-inflammatory meniscus bracket. The biological three-dimensional printing preparation method of the chondrocyte loading anti-inflammatory meniscus bracket can well simulate a three-dimensional macroscopic morphology, interior microcosmic collagen fibers and a porous structure of meniscus tissues, is beneficial to cell growth and exchange of nutrients and promotes regeneration of the meniscus tissues. For the biological three-dimensional printing preparation method of the chondrocyte loading anti-inflammatory meniscus bracket, by selecting polycaprolactone to print a frame structure of the meniscus bracket, enough mechanics is provided to joint support; meanwhile, by loading chondrocytes, operation of cell culture in vitro after bracket printing is avoided; and by loading a fullerene material, the bracket has the anti-inflammatory effect.

Description

Technical field [0001] The invention relates to the field of biological three-dimensional printing meniscus stents, in particular to a biological three-dimensional printing preparation method of anti-inflammatory meniscus stents loaded with chondrocytes. Background technique [0002] Nowadays, meniscus injury is a very common disease. In the United States, more than 1 million people receive meniscus surgery every year. The meniscus is a cartilage tissue, and lack of blood supply results in poor self-healing ability. For severely damaged meniscus, the clinical treatment of meniscus is usually used. Although meniscus resection has the advantages of good short-term curative effect and fast functional recovery, the quality of the regenerated meniscus after meniscus resection cannot perform the biomechanical function of normal meniscus, and it will inevitably aggravate articular cartilage degeneration in the long term. Allogeneic meniscus transplantation can replace the missing men...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/38A61L27/54A61L27/56B33Y10/00B33Y70/00B33Y80/00
CPCA61L27/18A61L27/3817A61L27/3852A61L27/54A61L27/56A61L2300/108A61L2300/41A61L2430/06B33Y10/00B33Y70/00B33Y80/00C08L67/04
Inventor 乔之光孙彬彬戴尅戎刘永佳倪兵王友
Owner SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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