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Preparation of FGF(fibroblast growth factor)-21 analogue and application to thrombus treatment

A technology of analogs and thrombus, which is applied in the field of biomedicine and can solve problems such as the inability to inactivate tPA activity

Active Publication Date: 2018-08-17
JIANGSU KANIONREAL BIOMEDICAL TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fibrinolytic activity of plasma is closely related to the action of plasminogen activator (tPA) and plasminogen activator inhibitor (PAI). Under physiological conditions, tPA is difficult to activate PLG, but when the fibrin content reaches a certain level PAI can enhance the effect of tPA, and PAI cannot inactivate tPA activity, leading to the activation of fibrinolytic system and inhibiting thrombus formation

Method used

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  • Preparation of FGF(fibroblast growth factor)-21 analogue and application to thrombus treatment
  • Preparation of FGF(fibroblast growth factor)-21 analogue and application to thrombus treatment
  • Preparation of FGF(fibroblast growth factor)-21 analogue and application to thrombus treatment

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Experimental program
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Effect test

Embodiment 1

[0112] Embodiment 1, the preparation of FGF-21 analog

[0113] 1. Sequence design of FGF-21 analogs

[0114] The NCBI retrieved human FGF21 sequence was spliced ​​with the elastin-like sequence, and the elastin-like amino acid sequence was optimized on the basis of the article (Urry DW et al., Biochim Biophys Acta.1974Dec 18; 371(2):597-602), specifically The sequence is 8 VPGAG pentapeptide repeats plus 12 VPGVG pentapeptide repeats. Finally, the sequence of the analog of the FGF-21 protein (abbreviated as FGF-21 analog) is obtained, as shown in SEQ ID No.1 (positions 1-183 are the sequence of the FGF-21 protein; positions 184-283 are elastinoids sequence); the coding gene of the FGF-21 analogue is shown in SEQ ID No.2 (the 1-549th position is the FGF-21 protein coding gene sequence; the 550-849th position is the elastin-like protein coding gene sequence).

[0115] 2. Construction of recombinant plasmids

[0116] 1. Synthesize the gene sequence designed in step 1 ("catatg+...

Embodiment 2

[0133] Embodiment 2, the therapeutic effect of FGF-21-like elastin on thrombus

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Abstract

The invention discloses preparation of fibroblast growth factor-21(FGF-21) analogue and application to thrombus treatment. The invention provides application of the fibroblast growth factor-21 analogue to any one of the following fields: (A) preparation of products for treating and / or preventing thrombus related diseases; and (B) treating and / or preventing thrombus related diseases. The inventionproves that the fibroblast growth factor-21 analogue (namely fusion protein formed through fusion of fibroblast growth factor-21 and elastin-like polypeptide) can effectively prevent thrombus from occurring, can serve as a drug to treat and / or prevent thrombus, and has the significant value on treatment and prevention of thrombus.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the preparation of a fibroblast growth factor-21 (FGF-21) analogue and its application in the treatment of thrombus. Background technique [0002] The formation of thrombus is a semi-solid clot formed after the coagulation of blood components in the circulating blood when the blood coagulation system is activated to varying degrees under physiological or pathological conditions. Thrombus is composed of insoluble fibrin, deposited platelets, accumulated white blood cells, and trapped red blood cells, causing partial or complete blockage of the blood vessel cavity, resulting in poor blood flow and obstruction of blood supply to the corresponding parts, which can cause ischemia and ischemia in the corresponding tissues or organs. Oxygen, necrosis, thereby affecting organ function and even life-threatening. [0003] Thrombosis can occur in any part of the blood vessel, which belongs to ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/18A61P7/02A61P29/00C07K14/50C12N15/70
CPCA61K38/1825A61P7/02A61P29/00C07K14/50C12N15/70
Inventor 李德山李帅孙旭王文飞任桂萍
Owner JIANGSU KANIONREAL BIOMEDICAL TECH CO LTD
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