Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Low-molecular-weight polysaccharide sulfate and preparation method and application thereof

A technology of diester sodium alginate and low molecular weight, which is applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, organic active ingredients, etc., can solve problems such as adverse reactions and weakness, and achieve improved solubility, reduced bleeding side effects, and prolonged Effects of clotting time and bleeding time

Active Publication Date: 2013-06-26
MARINE BIOMEDICAL RES INST OF QINGDAO CO LTD
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because of this, PSS is relatively weak in basic research on the material basis of its drug effects, quality control, and structure-activity relationship, which leads to the diversity of drug effects in the clinical application of PSS, and even adverse reactions. PSS is playing its role Strong anticoagulant effect will produce side effects such as bleeding, thrombocytopenia and some allergic reactions

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Low-molecular-weight polysaccharide sulfate and preparation method and application thereof
  • Low-molecular-weight polysaccharide sulfate and preparation method and application thereof
  • Low-molecular-weight polysaccharide sulfate and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: the preparation of low molecular weight sodium alginate diester

[0021] Weigh 9 g of sodium alginate diester (weight average molecular weight is about 14 kDa) and dissolve it in 120 mL of distilled water to prepare a 7.5% sodium alginate diester solution. Add 0.1mol·L -1 FeSO 4 solution and 30% H 2 o 2 aqueous solution, so that Fe in the reaction system 2+ and H 2 o 2 concentrations to achieve the specified final concentrations shown in Table 1. The closed shaking reaction was carried out at 60°C for 0.5h. After the reaction, according to 3mg·mL -1 The amount of reaction system Add ascorbic acid to consume H 2 o 2 The reaction was terminated and rapidly cooled, and 3 times the volume of 95% ethanol was added, and left standing overnight at 4°C. Centrifuge at 3000r·min-1 for 20min to separate the precipitate and supernatant. Dissolve the precipitate in a small amount of water, desalt and purify the sample with 732 cation exchange resin, and co...

Embodiment 2

[0029] Embodiment 2: The main pharmacological activity experiment of low molecular weight sodium alginate diester

[0030] The main pharmacological activity experimental results of the low molecular weight sodium alginate diester obtained by applying the method of the present invention are as follows, and the following results are all obtained through systematic experimental research according to the method stipulated by the state.

[0031] A: Anticoagulant activity test of low molecular weight sodium alginate diester

[0032] Insert the capillary tube into the venous plexus behind the inner canthus of the mouse to take blood, and record the time from blood collection to the appearance of coagulation filaments as the index for testing the coagulation time; after the tail of the mouse is docked, record the time from the natural outflow of blood to the natural cessation of blood To test the index of bleeding time. The above two indicators were used to detect and compare the ant...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
molecular weightaaaaaaaaaa
molecular weight distributionaaaaaaaaaa
Login to View More

Abstract

The invention provides a low-molecular-weight polysaccharide sulfate and a preparation method and application of the low-molecular-weight polysaccharide sulfate. The constitutional formula of the low-molecular-weight polysaccharide sulfate includes that the molecular skeleton of the constitutional formula is a binary linear block polymer composed of beta-D-(1,4)-mannuronic acid and alpha-L-(1,4)-guluronic acid. In the constitutional formula, R=Na or CH2CHOHCH3, R1=H or SO3Na, in each saccharide link, at least one R1=SO3Na, m+n is smaller than or equal to 25, the weight-average molecular weight of the low-molecular-weight polysaccharide sulfate ranges from 3kDa to 9kDa, and dispersion of molecular weight distribution ranges from 1.20 to 1.70. The low-molecular-weight polysaccharide sulfatecan be applied to treatment of anticoagulation and anti-thrombus diseases, reduces risk of haemorrhagia and avoids anaphylaxis, and supplies raw materials for wide bioactivity of a series of low-molecular-weight PSS (polysaccharide sulfate) products.

Description

technical field [0001] The invention relates to the field of antithrombotic and anticoagulant drugs, in particular to a low molecular weight sodium alginate diester and its preparation method and application. Background technique [0002] Sodium alginate (Propylene glycol alginate sodium sulfate, PSS) is based on the oligomeric sodium alginate molecule, and is modified by introducing propylene glycol ester groups and sulfonyl groups on the 6-position carboxyl group and the 2 and 3-position hydroxyl groups of the sugar ring, respectively. A class of heparin-like drugs obtained, wherein the weight-average molecular weight of the PSS bulk drug is generally 10-20kDa, the molecular weight distribution width is <1.80, and the organic sulfur content is 9%-13%. As a heparin-like drug, PSS has the functions of lowering blood viscosity, anticoagulation, lowering blood lipids, and improving microcirculation, and plays an important role in the prevention and treatment of hypercoagula...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/734C08B37/04A61P7/02
Inventor 李春霞管华诗孙杨李海花何小溪于广利
Owner MARINE BIOMEDICAL RES INST OF QINGDAO CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products