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Cordyceps sinensis mycelium, and fermentation culture medium and fermentation method for cordyceps sinensis mycelium

A technology of Cordyceps sinensis mycelium and fermentation medium, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of high production cost, low yield, and low content of key nutrients

Inactive Publication Date: 2018-08-10
HANGZHOU ZHONGMEI HUADONG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the mycelium of Cordyceps sinensis is mainly cultivated by submerged fermentation culture, and the yield is generally low. When the fermentation culture is completed, the ratio of the dry weight of the mycelium to the volume of the fermentation liquid is only 1%, the production cost is relatively high, and the content of key nutrients is relatively high. Low

Method used

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  • Cordyceps sinensis mycelium, and fermentation culture medium and fermentation method for cordyceps sinensis mycelium
  • Cordyceps sinensis mycelium, and fermentation culture medium and fermentation method for cordyceps sinensis mycelium

Examples

Experimental program
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preparation example Construction

[0029] Any method known in the art can be used to prepare the Cordyceps sinensis fermentation medium of the present invention. Here are only the following exemplary methods: prepare the solid substrate and nutrient solution (or water) in an appropriate ratio; use a glass triangular flask to contain the above screening medium, wrap the bottle with cotton gauze, sterilize at 121°C for 30 minutes, and cool To room temperature.

[0030] The conditions or environment suitable for culture in the present invention include a temperature of 12-18°C and a pH value of 5.5-7.0.

[0031] The culture or fermentation method of the present invention is not limited, and any technique or method known in the art can be used. The present invention only exemplarily enumerates the following methods.

[0032] Take the cordyceps freshly collected on the Qinghai-Tibet Plateau, separate and screen it on a specific screening medium for 3 generations to obtain a pure strain free of contaminants. The pure stra...

Embodiment 1

[0036] Example 1 Experiment on the effect of sorbose on the yield of mycelium and mannitol

[0037] The basic medium is formulated based on the following formula:

[0038] Soybean powder 2%, yeast extract powder 2%, glucose 2%, magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.02%, the balance water, a total of 100%.

[0039] Then, the basal medium was added with different contents of sorbose as shown in Table 1 below to prepare different medium. And use these media to ferment mycelium. Determine the dry weight and cordycepic acid content of different mycelium. The results are shown in Table 1 below.

[0040] Table 1 The effect of different concentrations of sorbose on the obtained mycelium

[0041] Program

Embodiment 2

[0042] Example 2 Experiment on the influence of Angelica sinensis extract on the obtained mycelium

[0043] The basic medium described in Example 1 was added with the homemade Angelica sinensis extract with different contents shown in Table 2 below to prepare different mediums. And use these media to ferment mycelium. Determine the dry weight and ash% of different mycelium. The results are shown in Table 2 below.

[0044] Table 2 Effect of Angelica extract on the mycelium

[0045] Program

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Abstract

The invention discloses a fermentation culture medium and a culture method for a cordyceps sinensis mycelium. The fermentation culture medium for the cordyceps sinensis mycelium further contains sorbose, sea cucumber extract, radix angelicae sinensis extract and soybean meal on the basis of the traditional culture media. As compared with natural cordyceps sinensis, the fermentation culture mediumand the culture method have the advantages that the contents of cordyceps polysaccharides can be increased by the fermentation culture medium and can reach 7.5% by weight, the content of cordycepic acid can reach 8.0%, and the dry weight of the cordyceps sinensis mycelium can be obviously increased and can reach 25 g / L; indexes of amino acid, adenosine, heavy metal and the like in mycelium powderare superior to indexes of amino acid, adenosine, heavy metal and the like in the natural cordyceps sinensis.

Description

Technical field [0001] The invention relates to cordyceps mycelium and its fermentation medium and fermentation method. Background technique [0002] Cordyceps belongs to the fungus species of the ergot family and Cordyceps. Its Latin name is Cordyceps sinensis, also written as phiocordyceps sinensis, and the name of the fungus is Hiptera spores, also known as Chinese spores. Cordyceps has a long history of medicinal use in China and Asian countries. It is a precious Chinese medicine tonic. Its healing and health effects have been recorded from the ancient medical book "Materia Medica" to the contemporary "Chinese Pharmacopoeia". The natural growth area of ​​Cordyceps is distributed in high altitude areas of Qinghai-Tibet Plateau such as Qinghai and Tibet. Due to predatory exploitation, resources and the environment have been severely damaged, and product prices have continued to rise. [0003] Cordyceps has a high nourishing health value, but natural Cordyceps is very expensive....

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 王鸿艳王丽琴张薇傅立峰
Owner HANGZHOU ZHONGMEI HUADONG PHARMA
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