Protein of gracilaria chouae glucophosphomutase and encoding gene and application of protein

A technology of glucose phosphate and coding gene, which is applied in the field of Gracilaria brittle phosphoglucomutase protein and its coding gene, and can solve the problems of lack of coding gene and application, etc.

Active Publication Date: 2018-08-07
OCEAN UNIV OF CHINA
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0005] At present, there is a lack of a protein of Gracilaria brittle phosphoglucomutase with high activity, its coding gene and its use

Method used

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  • Protein of gracilaria chouae glucophosphomutase and encoding gene and application of protein
  • Protein of gracilaria chouae glucophosphomutase and encoding gene and application of protein
  • Protein of gracilaria chouae glucophosphomutase and encoding gene and application of protein

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Embodiment 1

[0035]Trehalose is a non-reducing disaccharide formed by combining two glucose molecules through α,α-1,1-glycosidic bonds. The most widely existing synthetic pathway in organisms is uridine diphosphate glucose pyrophosphorylase (UDP-glucosepyrophosphorylase, UGP) catalyzed 1-phosphate glucose (Glucose-1-P, G-1-P) to produce uridine diphosphate Phosphoglucose (UDP-glucose, UDPG). UDPG and 6-phosphate glucose (Glucose-6-P, G-6-P) produced by phosphoglucomutase (Phosphoglucomutase, PGM) catalyzed G-1-P are used as substrates together, and trehalose synthase (Trehalose-6-P-synthase, TPS) catalyzed reaction to synthesize trehalose. This pathway has been discovered and validated in bacteria, fungi and plants. G-6-P can be converted into various forms of sugars in vivo, including free glucose, other monosaccharides, disaccharides, cell wall polysaccharides, starch, etc., and its chemical modification and allosterism play an important role in the whole biological sugar metabolism. ...

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Abstract

The invention discloses a protein of gracilaria chouae glucophosphomutase and an encoding gene and an application of the protein. The protein of the gracilaria chouae glucophosphomutase comprises a protein GchPGM1 and a protein GchPGM2, the amino acid sequence of the protein GchPGM is as shown in a sequence table SEQ ID NO.2, and the amino acid sequence of GchPGM2 is as shown in a sequence table SEQ ID NO. 4. The nucleotide sequence of the encoding gene GchPGM1 of the protein GchPGM1 of the gracilaria chouae glucophosphomutase is as shown in sequence table SEQ ID NO.1. The trehalose metabolicpathway, functions and effects of trehalose in aspects of system development, environmental adaptation and the like are further deeply known and mastered favorably; and the gene is used as a label toscreen or breed seaweed with high trehalose content, or genetic engineering seaweed with high trehalose content is constructed by over-expression of the gene.

Description

technical field [0001] This field relates to the technical field of algae cell engineering, especially relates to a protein of Gracilaria brittle phosphoglucomutase and its coding gene and application. Background technique [0002] Trehalose (Trehalose) is ubiquitous in bacteria, fungi, insects, invertebrates, plants and other biological kingdoms. In addition to being used as energy and carbon source storage in organisms, it is also an important stress-resistant protection substance, which can Protects proteins and cell membranes from damage in stressful environments including desiccation, dehydration, high or low temperature (Elbein et al., 2003). Macroalgae generally have high salt tolerance due to their special and diverse intertidal habitats. During the ebb and flow of the tide, it can still maintain good vitality under the stress of frequent extreme conditions such as high temperature, dry sun, osmotic shock, etc., and the trehalose contained in it is considered to be ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/12C12N9/90C12N15/54C12N15/60C12N15/70
CPCC07K2319/21C12N9/1205C12N9/90C12N15/70C12Y207/0101C12Y504/02002C12Y504/02005C12Y504/02006
Inventor 池姗刘涛刘翠
Owner OCEAN UNIV OF CHINA
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