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Method for improving heparin sensitivity of activated partial thromboplastin time reagent and application

A technology of thromboplastin time and reagents, applied in biological testing, material testing, etc., can solve the problems of different heparin effects, loss of heparin, and inability to sensitively reflect changes in heparin concentration, so as to prolong clotting time and improve sensitivity. sexual effect

Active Publication Date: 2018-07-31
SHANGHAI LONG ISLAND BIOTEC CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in practical applications, different components of APTT will lead to different effects of heparin on it, and generally cannot sensitively reflect changes in heparin concentration
The normal reference range of the APTT value of normal plasma without adding heparin is between 20 and 40 seconds, and the blood concentration of heparin used in clinical practice is usually between 0.2 and 0.8 U / mL. The APTT value measured by the existing APTT reagent , can not sensitively reflect the change of heparin concentration in the blood, thus losing the significance of clinical evaluation of heparin therapeutic effect

Method used

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Examples

Experimental program
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Embodiment

[0030] In this example, soluble metal salts of manganese ions or magnesium ions are added to the APTT reagent, and then the plasma is detected. Wherein, the activator of APTT reagent is ellagic acid or kaolin. The APTT reagent preparation method of this example is as follows:

[0031] Weigh 1.0g of kaolin or ellagic acid and dissolve it in 1000mL 20mM pH7.5 Tris-HCl buffer solution, stir for 30min until the whole liquid presents a uniform white suspension, then add 2.0g of phospholipid, mix well, and keep warm at 37°C for 1h. Finally, polyethylene glycol, gelatin and sodium azide are added in proportion, and then divalent metal salts of manganese ions or magnesium ions are added, mixed evenly, and the liquid APTT reagent is obtained. Sodium azide is a commonly used preservative, but other preservatives can also be used in this application. TRIS is a commonly used buffer salt, and other buffer salts that can maintain the pH at 6-8 can also be used in this application.

[003...

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PUM

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Abstract

The invention discloses a method for improving the heparin sensitivity of an activated partial thromboplastin time reagent and application. The method provided by the invention comprises the followingstep: adding a soluble manganese ion and / or magnesium ion metal salt into a reaction system when the activated partial thromboplastin time reagent is used for detecting plasma; or adding the solublemanganese ion and / or magnesium ion metal salt into the activated partial thromboplastin time reagent in advance before application of the reagent to activate partial thromboplastin time detection. According to the method, the heparin sensitivity of the activated partial thromboplastin time reagent is improved by adding the metal salt of soluble manganese ions and / or magnesium ions, so the activated partial thromboplastin time reagent can more sensitively reflect the changes of a heparin concentration. The invention also discloses the application of the soluble manganese ion or magnesium ion metal salt to the preparation of the activated partial thromboplastin time reagent sensitive to heparin.

Description

technical field [0001] The application relates to the field of blood detection reagents, in particular to a method for improving the sensitivity of activated partial thromboplastin time reagents to heparin, and the use of soluble manganese ion or magnesium ion metal salts in the preparation of activated partial thromboplastin time sensitive to heparin Use in time reagents. Background technique [0002] The detection of activated partial thromboplastin time, namely APTT (Activated Partial Thromboplastin Time), is the most commonly used method for screening the endogenous coagulation system, and it is used by medical institutions as an auxiliary diagnosis. The detection principle is: adding activated partial thromboplastin time reagent to the plasma to be tested, mixing and pre-warming at 37°C for a certain period of time, with the participation of calcium ions, the endogenous coagulation system is activated to convert fibrinogen into insoluble fibrin, and the coagulation is m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/86
CPCG01N33/86
Inventor 江明朱斌斌
Owner SHANGHAI LONG ISLAND BIOTEC CO LTD
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