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Marker for glioblastoma, application thereof and kit

A technology for glioblastoma and glioma, applied in the field of tissue tumor markers

Active Publication Date: 2018-07-31
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current treatment methods can only slightly prolong the survival period of patients, and the diagnosis must be confirmed by comprehensive medical history, auxiliary diagnosis and postoperative pathological diagnosis. Therefore, the development of new treatment methods and diagnostic markers has become an urgent need for the prevention and treatment of glioblastoma. need

Method used

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  • Marker for glioblastoma, application thereof and kit
  • Marker for glioblastoma, application thereof and kit
  • Marker for glioblastoma, application thereof and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: Through the data analysis of TCGA database, mine the relationship between METTL3 gene expression and the survival rate of glioblastoma patients.

[0022] The TCGA standard method was used to download the expression profile microarray data of two groups of glioblastoma tissue samples and the survival status of clinical follow-up patients, and statistical software was used to draw the relationship between the expression level of METTL3 and the overall survival rate of glioblastoma patients.

[0023] Result: see figure 1 , the expression of METTL3 was significantly correlated with the survival rate of glioblastoma patients, and the overall survival rate of patients with high expression of METTL3 was significantly lower than that of patients with low expression of METTL3, suggesting that METTL3 may play a role in promoting cancer development.

Embodiment 2

[0024] Example 2: mining the relationship between the expression of METTL3 and the overall survival rate of glioma patients through the database.

[0025] The TCGA database was used to download the transcriptome RNA-seq sequencing data of glioma patients, and the expression level of METTL3 in different samples was analyzed.

[0026] Result: see figure 2 A, analysis found that compared with normal brain tissue, the expression level of METTL3 in glioma was significantly increased, which can be considered as an early diagnostic indicator for glioma.

Embodiment 3

[0027] Example 3: RT-qPCR verification of the expression difference of METTL3 gene in glioma samples and normal brain tissues.

[0028] By measuring the METTL3 gene expression status in normal brain tissue cells and tumor tissues of patients with glioma, 10 tissue samples in the control group were obtained from patients with traumatic brain tissue. Glioma tissue was obtained through neurosurgical resection and confirmed by pathological diagnosis.

[0029] First, total cellular RNA was extracted. Keep all operations and related reagents on ice. Use Trizol reagent to extract total RNA from cells, and the specific steps are as follows: Prepare to extract total RNA. Cells were directly digested and lysed with Trizol, and Trizol was added. After the cells were added with Trizol, they were left at room temperature for 5 min to fully lyse. Use a high-speed refrigerated centrifuge to centrifuge at 12,000 rpm at 4°C for 5 minutes, and discard the precipitate. Add chloroform at 200u...

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Abstract

The invention discloses a marker METTL3 (Methyltransferase Like 3) for glioblastoma and application thereof. The METTL3 is obviously highly expressed in a glioma cell; further, the extent of the expression of the METTL3 has obvious relevance with a clinical scale; the METTL3 is most highly expressed in the glioblastoma with the highest malignant degree; the expression of the METTL3 is knocked downto be capable of inhibiting the growth of the glioblastoma, and the METTL3 is prompted to be capable of becoming a target spot for treating the glioblastoma.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to a tissue tumor marker related to glioblastoma and an application thereof. Background technique [0002] Glioma is the most common primary intracranial malignancy. Children and adolescents are prone to onset, and the progress is rapid, the course of the disease is short, and the brainstem can be involved in a short period of time, and the condition is dangerous; the course of the disease is long and the progress is slow in adults, because there are no obvious symptoms at the beginning of the development, the tumor has grown to the size of the corresponding tumor by the time the patient notices it. brain area. Surgical resection causes brain tissue damage, coupled with the characteristics of difficult resection and infiltrating tumor growth, the prognosis of glioma is often poor. At present, the treatment of glioma is mainly based on surgery, supplemented by radiotherapy and ch...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886G01N33/574A61K45/00A61P35/00
CPCA61K45/00A61P35/00C12Q1/6886C12Q2600/118C12Q2600/136C12Q2600/158G01N33/57484G01N2800/52
Inventor 赵蔚易旸李福喜
Owner SUN YAT SEN UNIV
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