Gene chips for skin gene detection

A gene chip and gene detection technology, applied in the field of gene detection, can solve the problems of low accuracy and few detection sites, and achieve the effects of high accuracy, many detection sites, and strong practicability

Pending Publication Date: 2018-07-31
广州中安基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Patent 201710964440.2 discloses a gene detection kit, including freckle gene chips, acne gene chips, and skin color gene chips. Although this patent detects freckle and acne-related genes and achieves the effect of facilitating personalized customized preventive medical solutions, its detection Few sites, low accuracy

Method used

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  • Gene chips for skin gene detection
  • Gene chips for skin gene detection

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] 1. Kit components

[0052] A skin gene detection kit, including anti-sun spots gene chip, multiplex PCR amplification primer set 1, anti-freckle gene chip, multiplex PCR amplification primer set 2, anti-tanning gene chip, multiplex PCR amplification primer set 3, Hybridization solution and washing solution.

[0053] 1. Anti-sun spot gene chip

[0054] Includes solid support (nylon membrane) and probe set A. The probe group A includes probes 1-6, and the sequences of the probes in the probe group A are shown in SEQ ID NO: 1-6.

[0055] 2. Anti-freckle gene chip

[0056] Includes solid support (nitrocellulose membrane) and probe set B. The probe group B includes probes 1-10, and the sequences of the probes in the probe group B are shown in SEQ ID NO: 1-10.

[0057] 3. Anti-tanning gene chip

[0058] Includes solid support (polystyrene) and probe set C. The probe group C includes probes 1-2 and probes 9-18, and the sequences of the probes in the probe group C are sh...

Embodiment 2

[0071] Sample screening: Randomly select subjects with sun spots, freckles or dark skin and normal skin on the face, obtain oral epithelial cells through cotton swabs, extract genomic DNA, and use genomic DNA as a template to amplify IRF4 gene, MC1R gene, BNC2 gene, TYR gene, OCA2 gene, SLC24A5 gene, HERC2 gene and SLC45A2 gene were subjected to Sanger sequencing. IRF4 gene amplification primers are 19-20, the primer sequence is shown in SEQ ID NO: 19-20, MC1R gene amplification primers are 21-24, the primer sequence is shown in SEQ ID NO: 21-24, BNC2 gene amplification The primers are 25-26, the primer sequence is as shown in SEQ ID NO: 25-26, the TYR gene amplification primers are 27-28, the primer sequence is as shown in SEQ ID NO: 27-28, and the OCA2 gene amplification primer is 29-30, The primer sequence is shown in SEQ ID NO: 29-30, the SLC24A5 gene amplification primer is 31-32, the primer sequence is shown in SEQ ID NO: 31-32, the HERC2 gene amplification primer is 33-...

Embodiment 3

[0092] Embodiment 3 verification experiment

[0093] 1) Questionnaire

[0094]Before the genetic test, a questionnaire will be conducted to understand the facial conditions of the subjects. The specific questions of the questionnaire mainly include age, gender, skin quality, whether there is stress or smoking in life, alcoholism, staying up late, frequent exposure to radiation, The status of heavy metals, whether there are spots and the type of spots, skin color, skin care products used, whether sunscreen is used, etc. The results of the questionnaire can be checked for follow-up testing and preventive measures can be customized for each individual.

[0095] One of the subjects (Ms. Zhang) was selected through the questionnaire for subsequent verification experiments.

[0096] 2) Actual observation

[0097] a. Through the observation of Ms. Zhang after cleansing, it can be clearly seen that Ms. Zhang's overall skin color is a dark wheat color, and there are obvious yellow-b...

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Abstract

The invention provides gene chips for skin gene detection and a kit thereof. The gene chips comprise the anti-sun-spot gene chip, the anti-freckle gene chip and the anti-sunburn gene chip. The kit comprises the gene chips, the anti-sun-spot capacity, the anti-freckle capacity and the anti-sunburn capacity of an individual can be quickly detected, genetic information of the spot generation resistance of the individual is obtained before generation of sun spots and freckles, the chips also help the individual to know about genetic information of skin color change of the individual, the individual is guided in taking related prevention measures, and the powerful support is provided for individualized medical beauty. The gene chips are adopted for detection, multiple detection genetic loci exist, in combination with questionnaires and actual observation, the sensitivity of a detection effect is good, the accuracy rate is high, the practicability is high, sampling is conducted conveniently,and the gene chips are conveniently popularized and applied to the beauty industry.

Description

technical field [0001] The invention relates to the technical field of gene detection, in particular to a skin gene SNP detection carrier chip and related detection kits. Background technique [0002] Genetic testing refers to the technology of detecting genomic DNA through blood, oral mucosa cells or other tissue cells. After extracting the DNA in the cells and amplifying the relevant genes, the genetic information in the cells is detected by specific equipment, and the genotype information contained in it is analyzed, so that people can understand the difference between themselves and others from the genetic level, so as to use targeted Means to prevent or ameliorate the possible effects of related genes. [0003] Each person's somatic cell nucleus contains 23 pairs of chromosomes composed of DNA and ribosomal proteins. There are about 3 billion base pairs on these chromosomes. DNA sequence polymorphisms caused by single nucleotide variations at the genome level Sex is c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/6858C12Q1/6837
CPCC12Q1/6837C12Q1/6858C12Q1/6883C12Q2600/156C12Q2600/16C12Q2531/113C12Q2537/143
Inventor 陈茜张欣鑫张凯
Owner 广州中安基因科技有限公司
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