New target using curcumin to inhibit hepatic cancers and application

Abstract

The invention belongs to the technical field of medical treatment, and discloses a new target using curcumin to inhibit DAMP (damage associated molecular pattern) molecule HSP70 and TLR4 signals to inhibit hepatic cancers and application. By establishing a cell culture and cell heat stress model, the statistical analysis is performed by CCK-8 test, scratch experiment, Transwell transfer experiment, cell cycle analysis by a flow cytometry, cell withering analysis by the flow cytometry, Western blot analysis, enzyme linked immunosorbent assay and other methods. Proofed by the statistical analysis result, the application has the advantages that the level of DAMP molecule HSP700 outside the cell and the expression of TLR4 protein are obviously increased by the heat stress, and the expression of DAMP molecule HSP700 inside the cell is obviously reduced; the proliferation, invasion and transfer of human hepatoma cell line HepG2 cells are inhibited by the curcumin, the cell withering is promoted, the cell is blocked at the S period, and the heat-stress level of the DAMP molecule HSP70 (eHSP70) outside the HepG2 cell is obviously reduced; after the curcumin is removed, the eHSP70 is increased again.

Description

technical field [0001] The invention belongs to the field of medical technology, and in particular relates to a new target and application for inhibiting liver cancer by using curcumin to inhibit DAMP molecules HSP70 and TLR4 signals Background technique [0002] Curcumin has been widely reported to have anti-inflammatory, antibacterial, antioxidant, antitumor, and even antineurodegenerative properties. The mechanism of action of curcumin is unknown, especially in inhibiting liver cancer. However, activated NF-κB signaling pathway is associated with liver cancer progression, and HSP70 can bind to TLR4 receptor to activate NF-κB. The "danger theory" of immunology shows that DAMPs are related to inflammation, autoimmune diseases, tumors and various aging diseases. Extracellular HSP70 is a typical DAMP molecule, which can stimulate the immune system to generate an immune response as an endogenous immune stimulator. Many aging-related diseases share common origins and characte...

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Problems solved by technology

[0003] To sum up, the problems existing in the existing technology are: it has been reported that Curcumin can inhibit the occurrence of liver cancer, curcumin inhibits TLR4 signaling by inhibiting the DAMP molecule eHSP70, thereby inhibiting NF-κB is a technical blank

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