Method for quickly testing praziquantel enantiomer composition based on near infrared spectroscopy

Abstract

The invention discloses a method for detecting the content of a praziquantel enantiomer, and particularly relates to a method for establishing a quantitative model to detect the content of the praziquantel enantiomer in a solution by combining a PLS (Partical Least Square method) based on a near infrared spectroscopy. The method comprises the following steps: after performing smoothing processingon collected spectral information through an SG smoothing filter, comprehensively analyzing SG (5,5) at the two wave bands of 1816.9-1884.3 nm and 1405.3-1425.4 nm through a second-order derivative treatment, and establishing a good model. The method can realize quick and undamaged detection sample, is high in sensitivity, and is applicable to production workshops. Based on the method, the invention further provides an infrared spectroscopy modeling toolkit for testing the praziquantel enantiomer composition and application of the infrared spectroscopy modeling toolkit in a praziquantel medicine analysis field. The infrared spectroscopy modeling toolkit comprises a spectrum file access integration module, an image preprocessing module, a wave band optimization module, a modeling module anda model evaluation module.

Description

technical field [0001] The invention relates to the field of drug analysis, in particular to a method for detecting the composition of praziquantel enantiomers in a sample based on near-infrared spectrum regression modeling. Background technique [0002] Schistosomiasis is a common, zoonotic, and serious parasitic disease. Praziquantel (PZQ) is currently the drug of choice for the treatment of schistosomiasis, with broad-spectrum anti-blood-sucking and anti-tapeworm pharmacological effects. Praziquantel belongs to pyrazinoisoquinoline compounds, is a chiral compound and has two enantiomers. Studies have found that the left-handed isomer is the main form of drug efficacy, while the right-handed isomer will produce certain toxic and side effects. Therefore, the content determination of the enantiomer of praziquantel is of great significance to its quality control. [0003] At present, the most commonly used method for the determination of enantiomers of chiral drugs is high ...

AI Technical Summary

Problems solved by technology

[0005] (1) The analysis time of liquid chromatography is relatively long and time-consuming;

[0006] (2) Since the sample needs to be prepared into a solution for chromatography, and some also need to introduce other reagents, it will cause loss of the original sample;

[0007] (3) The sensitivity of liquid chromatography is low;

[0008] (4) Liquid chromatography works offline and cannot be monitored in real time during the production of drugs

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