Biological sample preparation method and application thereof and qualitative and quantitative metabolite analysis method

Abstract

The invention provides a biological sample pretreatment method for treating a biological sample to obtain a pretreatment sample before gas chromatography and mass spectrometer combined detection and analysis on a metabolite in the biological sample from a living organism, application thereof to metabolite detection, and a corresponding metabolite detection and analysis method. The biological sample pretreatment method comprises the following steps: 1, performing extracting treatment; 2, performing a derivative reaction: performing the derivative reaction on the extracted biological sample by using an auxiliary reagent and a derivatization reagent to obtain a derivative biological sample; 3, standardizing and removing impurities, wherein the auxiliary reagent at least comprises a strong alkaline solution and a low-temperature alcohol organic solvent; in the step 1, the strong alkaline solution is used first and then the low-temperature alcohol organic solvent is used for continuously extracting the biological sample.

Description

technical field [0001] The invention belongs to the field of chemistry, and in particular relates to a biological sample used for processing biological samples to obtain pre-treated samples before performing gas chromatography and mass spectrometry detection and analysis on metabolites in biological samples from living organisms The preparation method and its application in the qualitative and quantitative analysis of metabolites, and the corresponding qualitative and quantitative analysis methods of metabolites. Background technique [0002] There are a large number of metabolites produced by various pathways in organisms, some are produced by the metabolism of the organism itself, some are produced by the metabolism of the organism and the microbiota in the organism, and some are produced by the microbiota in the organism produced by its own metabolism. The metabolites produced by each pathway exert their respective functions and cooperate with each other to affect the ov...

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Problems solved by technology

[0003] The concentration of metabolites produced by various pathways varies greatly, and their physical and chemical properties also vary widely, especially the metabolites produced by the intestinal bacteria of the human body (organism) in the organism (host), and the relationship between intestinal bacteria and the organism (host). ) co-metabolites, including short-chain fatty acids, amino acids, benzoyl and phenyl derivatives, indole derivatives, lipids, bile acids, choline, vitamins and polyamines, etc., which are quite different Physical and chemical properties, so how to simultaneously measure as many metabolites as possible with as few platforms as possible is extremely challenging

[0004] At present, there is no method that can simultaneously detect short-chain fatty acids, amino acids, benzoyl and phenyl derivatives, indole derivatives, lipids, bile acids, choline, vitamins and polyamines, etc. molecular metabolites

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