Processing method and processing device of high-throughput sequencing data, storage medium and processor

Abstract

The invention provides a processing method and a processing device of high-throughput sequencing data, a storage medium and a processor. The processing method includes: acquiring secondary sequencingsequences, wherein the secondary sequencing sequences are sequencing sequences which are in the high-throughput sequencing data, can be recognized by amplification primers of a target fragment and areafter removing corresponding amplification primers; carrying out alignment on the secondary sequencing sequences and a reference genome sequence to obtain a primary variation result; and utilizing mutation data in known mutation data to correct the primary variation result to obtain a processing result. False positive processing results caused by primer mutation existing in amplification productoverlapping regions are reduced through partially removing the primers in all the sequences from the original data, which are obtained by high-throughput sequencing, according to known primer information. Certain erroneously-amplified sequences in the high-throughput sequencing data can also be removed, accuracy of subsequent analysis is improved, and decreasing of an overall data amount and improving of analysis efficiency are also facilitated.

Description

technical field [0001] The present invention relates to the field of high-throughput sequencing data processing, in particular, to a high-throughput sequencing data processing method, processing device, storage medium and processor. Background technique [0002] At present, there are many methods for gene sequencing to detect mutations. Among them, it is an efficient and economical common method to amplify specific target regions through multiple amplification and perform high-throughput sequencing on the products. However, the high-throughput sequencing process will generate a large amount of sequence information. Therefore, how to quickly and accurately process these sequencing data information has become an urgent technical problem to be solved. [0003] Although there are many processing and analysis methods for high-throughput sequencing data in the prior art, these methods have the defect of low accuracy of processing results. Therefore, there is still a need to impr...

AI Technical Summary

Problems solved by technology

[0004] The main purpose of the present invention is to provide a processing method, processing device, storage medium and processor for high-throughput sequencing data, so as to solve the problem of many false positive sites in the existing processing results

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