Method for shortening period of Luzhou-flavor liquor fermentation by external esterification
A technology of esterification of Luzhou-flavor liquor, which is applied in the field of brewing, can solve the problems of reducing production efficiency, increasing the production cost of Luzhou-flavor liquor, and low liquor yield, so as to increase the richness, shorten the fermentation cycle, and improve the aroma of the cellar. Elegant effect
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Embodiment 1
[0040] Example 1, a method for shortening the fermentation period of Luzhou-flavor liquor through esterification outside the pit, firstly through the cultivation of caproic acid compound acid liquid outside the pit, to obtain a compound acid liquid mainly composed of caproic acid; To increase the alcohol content of the liquid, add yellow water and esterification enzyme to obtain a mature esterified liquid through normal temperature and closed esterification outside the cellar; the mature esterified liquid is added to the bottom pot before being placed in a retort for string distillation.
Embodiment 2
[0041] Embodiment 2, a method for shortening the fermentation cycle of Luzhou-flavor liquor by esterification outside the pit, specifically comprises the following steps:
[0042] (1) Preparation of caproic acid mixed acid solution: caproic acid bacteria use caproic acid fermentation medium to carry out caproic acid fermentation, and leave to stand for airtight culture for 7 days to obtain caproic acid compound culture solution for esterification;
[0043] (2) Equipped with esterification solution: take the fermented hexanoic acid compound culture solution, add appropriate amount of yellow water, adjust the alcohol content of the mixed culture solution with high alcohol, add esterification enzyme, stir evenly, seal it, and esterify at room temperature for 24 hours to obtain Esterification solution;
[0044] (3) String steaming of Luzhou-flavor liquor: according to the brewing process of Luzhou-flavor liquor, the fermented Luzhou-flavor fermented grains fermented for 42 days ar...
Embodiment 3
[0045] Example 3, in the step (1) of a method for shortening the fermentation period of Luzhou-flavor liquor through esterification outside the cellar described in Example 2, the formula of the seed liquid culture medium w / w of caproic acid bacteria is: NaAc 0.6 %, Yeast Extract 0.5%, K 2 HPO 3 0.04%, MgSO 4 0.02%, (NH 4 ) 2 SO 4 0.05%, ethanol 2%, ethanol is added after the culture medium is sterilized; the culture method is: culture at 37°C, initial pH 7.0, liquid volume ≥ 90% submerged culture for 10 days.
[0046] The expansion and strengthening medium w / w formula used for the expansion of caproic acid bacteria seed liquid is: ethanol 2%, pit mud extract 0.5%, yeast extract 0.5%, Daqu powder 0.6%, culture at 37°C, initial pH 7.0. Caproic acid bacteria seeds were inoculated at 5% w / w and submerged for 5 days to achieve expanded cultivation.
[0047] The esterase described in step (2) is lipase, preferably a food-grade liquid super-concentrated lipase synthesized by...
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