Method for accurately determining chitosan content by resonance Rayleigh scattering method

Abstract

The invention relates to a method for accurately determining chitosan content by a resonance Rayleigh scattering method and belongs to the field of macromolecular substance detection. The method solves the problem that in the prior art the chitosan content of a finished product is difficult to determine and processes are complex. The method utilizes the characteristic of chitosan reacting with Allura Red under weakly acidic conditions to produce an association complex, and comprises measuring the RRS intensity IRRS of the ion association complex and the RRS intensity I0RRS of the reagent blankat the maximum RRS wavelength, calculating delta IRRS according to a formula of delta IRRS=IRRS-I0RRS, building standard curves of delta I and different molecular weight chitosan concentrations, selecting the standard curve according to the chitosan molecular weight in the sample, and calculating the chitosan content of the sample according to the delta IRRS of the detected sample. The method hasthe advantages of cheap reagents, high sensitivity and good reproducibility, and is suitable for promotion and application in practical applications.

Description

technical field [0001] The invention relates to a method for accurately measuring chitosan content by a resonance Rayleigh scattering method, belonging to the field of detection of macromolecular substances. Background technique [0002] Chitosan (chitosIn) is a degradation product of chitin. The chemical name is polyglucosamine (1,4)-2-amino-2-deoxy-D-glucan, which is the main component of the exoskeleton of crustaceans (shrimp, crab) and insects. Chitosan is found in nature It is widely distributed and belongs to the polymer green material. Human understanding of chitosan began in the 19th century, and the research on chitin / chitosan became increasingly active until the 1960s. Internationally, since 1894, 6 conferences on chitin / chitosan have been held; Asia also held the first academic conference on chitin in 1994, and now it has held 2 conferences. However, my country has only paid attention to the development and application of chitin / chitosan in recent years. In Oc...

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the technical deficiency that the content of chitosan in the finished product is difficult to quantify in the prior art, and operation technology is loaded down with trivial details, the present invention provides a kind of method that accurately measures chitosan content by resonant Rayleigh scattering method, the present invention utilizes chitosan Under weakly acidic conditions, it reacts with allura red to form an association, and measures the RRS intensity I of the ion association at the maximum RRS wavelength RRS and the RRS intensity of the reagent blank I 0RRS , to calculate ΔI RRS =I RRS -I 0RRS , establish the standard curve of ΔI and different molecular weight chitosan concentrations, then select the appropriate standard curve according to the molecular weight of chitosan in the sample, according to the ΔI of the sample measured RRS , so as to calculate the content of chitosan in the sample. The determination of this method is affected by the molecular weight of chitosan. It has the advantages of cheap reagents, high sensitivity and good reproducibility, and is suitable for popularization and use in practical applications.

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