Magnetic bead sealer for magnetic particle chemiluminescence diagnosis system

Abstract

The invention discloses a magnetic bead sealer for a magnetic particle chemiluminescence diagnosis system. The magnetic bead sealer comprises an aqueous solution of BSA, Gly, horse serum and triethanolamine. The final concentration of the BSA in the aqueous solution is 5-15 mg / mL, the final concentration of the Gly in the aqueous solution is 10-20 mg / mL, the final concentration of the horse serumin the aqueous solution is 100-300 ul / mL, and the final concentration of the triethanolamine in the aqueous solution is 50-150 ul / mL. The magnetic bead sealer of the magnetic particle chemiluminescence diagnostic system combines commonly used blocking agents for the first time, and has an unexpected technical effect. On the basis of ensuring already cross-linked proteins, the magnetic bead sealercan seal activated groups and non-specific adsorption sursurfaces on magnetic beads, not only does not change the high signal intensity of reaction in the prior art, but also ensures long-term continuous stability of a magnetic particle reagent, meanwhile has a lower background signal value, and can greatly improve test sensitivity and linear range.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnostic testing, in particular to a magnetic bead sealing agent for a magnetic particle chemiluminescence diagnostic system. Background technique [0002] The magnetic particle chemiluminescence diagnostic system needs to use magnetic bead blocking agents. The commonly used magnetic bead blocking agents in the prior art include BSA, serum, skimmed milk powder, casein, etc., but the conventional magnetic bead blocking agents are not satisfactory. [0003] BSA and serum are the most commonly used blocking agents, and a single component is suitable for most situations. However, if the immunogen is coupled to BSA, BSA and serum have strong immunogenicity, which will lead to a lot of antibodies against BSA and serum. To avoid cross-reaction, BSA serum, skim milk powder, etc. cannot be used to block, and large There are a small amount of antibody residues in some BSA and serum, which will lead to c...

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to overcome the single component of the existing magnetic bead sealant, which often has defects such as small usable range and high background, and provide a magnetic bead sealer for magnetic particle chemiluminescence diagnostic system