One-step embryo culture medium and preparation method thereof

Abstract

The invention belongs to the field of assisted reproduction, and in particular relates to a one-step embryo culture solution and a preparation method thereof. The composition used to prepare one-step embryo culture solution in the present invention, the composition is composed of (±)-α-lipoic acid, acetyl L-carnitine or acetyl L-carnitine hydrochloride, N-acetyl-L ‑cysteine ​​composition; the molar ratio of (±)‑α‑lipoic acid, acetyl L‑carnitine or acetyl L‑carnitine hydrochloride, N‑acetyl‑L‑cysteine ​​is 0.055‑ 0.098: 0.044‑0.078: 0.702‑0.858. The one-step embryo culture solution prepared by using the composition retains the active ingredients secreted by the embryo into the culture solution, can protect cells and inhibit the decomposition and utilization of harmful products, and avoid possible mechanical damage and culture environment during embryo transfer. The problem of low blastocyst formation rate caused by different stress responses.

Description

technical field [0001] The invention belongs to the field of assisted reproduction, and in particular relates to a one-step embryo culture solution and a preparation method thereof. Background technique [0002] In Vitro Fertilization-Embryo Transfer In Vitro Fertilization-Embryo Transfer, referred to as IVF-ET, refers to the removal of eggs and sperm, in vitro fertilization, and then the embryo precursor - fertilized eggs are transplanted back into the mother's uterus to develop into fetuses. Test-tube babies are babies born by artificially fertilizing eggs and sperm outside the body and carrying out early embryonic development, and then transplanting them into the mother's womb. [0003] Since the world's first "test-tube" baby was born in the UK on July 25, 1978, as of July 1, 2012, 5 million "test-tube" babies have been born. IVF has brought good news to more than 50 million infertile couples worldwide. However, more than three decades of clinical practice has not sign...

AI Technical Summary

Problems solved by technology

[0005] The nutrition of the currently used semen preparation is incomplete, or its physical or chemical indicators are too wide, the same test process, the same batch of semen may easily cause the death of sperm or egg cells during the culture process, or the sperm capacitation is insufficient and the motility is low, or Egg viability is compromised, as in a sperm motility test, with a low sperm viability or a rate close to or below the qualifying criteria (≥75% viability)

Some fertilized semen have a high consistency, which contains harmful ingredients and inflammatory cells are not removed cleanly, which seriously affects the development and growth of fertilized eggs

[0006] In addition, the effectiveness of the general culture medium cannot be visually judged before use. If it continues to be used after it has deteriorated, it will cause irreversible results to the test or clinical operation.

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