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Breeding swine frozen semen unfreezing diluent, and preparation method thereof

A technology of diluent and frozen semen, applied in the field of frozen semen thawing of pigs, can solve the problems of insufficient antioxidant capacity and inability to effectively resist oxidative damage of sperm, and achieve the effect of reducing oxidative damage and achieving good stability.

Active Publication Date: 2018-07-06
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The main problem of the current thawing diluent of pig frozen semen is insufficient antioxidant capacity, because the temperature of the frozen semen thawing process rapidly rises from minus 196 degrees Celsius to about 25 degrees Celsius within 20 seconds, which is a very drastic temperature change, here A large amount of active oxygen will be generated during the process, and the currently used frozen semen thawing diluent is added with antioxidant components according to the formula of fresh semen diluent, so it cannot effectively resist the oxidative damage of sperm during the recovery process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0019] The specific embodiment one, the proportioning of each component in the 1000mL thawed diluent in the present embodiment is:

[0020] D-glucose 15g, fructose 10g, potassium chloride 0.2g, edetate disodium 1g, sodium bicarbonate 3g, trisodium citrate 8g, polyvinyl alcohol 0.5g, gentamicin 500,000 IU, astragalus polysaccharide 4g, resveratrol 0.4g; The preparation method of above-mentioned pig frozen semen thawing diluent is carried out according to the following steps:

[0021] Step 1, D-glucose, fructose, potassium chloride, disodium edetate, sodium bicarbonate, trisodium citrate, and polyvinyl alcohol are prepared into an aqueous solution with double distilled water;

[0022] Step 2. Place in a 26°C water bath to balance until fully dissolved and the pH value is stable;

[0023] Step 3, filter with a 0.22 μm sterile filter;

[0024] Step 4: Add gentamicin, astragalus polysaccharide and resveratrol 30 minutes before use to obtain a diluent for thawing semen.

[0025] ...

specific Embodiment approach 2

[0026] Specific embodiment two, the proportioning of each component in the 1000mL thawing diluent in the present embodiment is:

[0027] D-glucose 20g, fructose 8g, potassium chloride 0.5g, edetate disodium 2g, sodium bicarbonate 2.5g, trisodium citrate 5.6g, polyvinyl alcohol 1g, gentamicin 400,000 IU, astragalus Polysaccharide 4g, resveratrol 0.46g.

[0028] The preparation method of the thawed diluent in this embodiment is carried out according to the method of Embodiment 1.

[0029] After testing, the pH value of the frozen semen thawing diluent in this embodiment is in the range of 7.2-7.5, and the osmotic pressure is in the range of 240mosM-380mosM.

[0030] Adopt following test to verify invention effect

[0031] Take out a tube of 0.5 mL frozen semen from liquid nitrogen, shake it in a water bath at 38 degrees Celsius for 20 seconds, and then dry it quickly. D-glucose 20g, fructose 8g, potassium chloride 0.5g, edetate disodium 2g, sodium bicarbonate 2.5g, trisodium ...

specific Embodiment approach 3

[0042] Specific embodiment three, the proportioning of each component in the 1000mL thawing diluent in the present embodiment is:

[0043]D-glucose 25g, fructose 5g, potassium chloride 1.0g, edetate disodium 3g, sodium bicarbonate 2g, trisodium citrate 5g, polyvinyl alcohol 2g, gentamicin 350,000 IU, astragalus polysaccharide 4g , Resveratrol 0.5g.

[0044] The preparation method of the thawed diluent in this embodiment is carried out according to the method of Embodiment 1.

[0045] After testing, the pH value of the frozen semen thawing diluent in this embodiment is in the range of 7.2-7.5, and the osmotic pressure is in the range of 240mosM-380mosM.

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Abstract

The invention discloses a breeding swine frozen semen unfreezing diluent, and a preparation method thereof, and belongs to the technical field of swine frozen semen unfreezing. The breeding swine frozen semen unfreezing diluent can be used for solving a problem that semen oxidation damage of swine frozen semen is easily caused in unfreezing diluting process. The breeding swine frozen semen unfreezing diluent is composed of D-glucose, fructose, potassium chloride, disodium ethylene diamine tetraacetate, sodium bicarbonate, trisodium citrate, polyvinyl alcohol, gentamycin, astragalus polysaccharide, and resveratrol. A preparation method comprises following steps: 1, D-glucose, fructose, potassium chloride, disodium ethylene diamine tetraacetate, sodium bicarbonate, trisodium citrate, and polyvinyl alcohol are prepared into an aqueous solution with double distilled water; 2, the aqueous solution is introduced into a water-bath kettle at 26 DEG C for balance treatment until full dissolvingis realized, and the pH value is stable; 3, a bacterial filter is adopted for filtering; 4, and gentamycin, astragalus polysaccharide, and resveratrol are added 30min before using. The breeding swinefrozen semen unfreezing diluent is capable of achieving oxidation resistance effectively, and reducing oxidation damage of violent stability change in frozen semen unfreezing process on semen.

Description

technical field [0001] The invention belongs to the technical field of thawing frozen semen of pigs, and in particular relates to a thawing diluent of frozen semen of pigs and a preparation method thereof. Background technique [0002] Ultra-low temperature cryopreservation technology breaks the limitation of fresh essence on time and space. Ultra-low temperature cryopreservation of pig semen can give full play to the genetic potential of high-quality boar population reproduction, speed up the improvement of livestock varieties, establish animal germplasm resource banks, and bring rapid development to the pig industry. It is an efficient and low-cost method. Porcine semen preservation pathway. On the other hand, as a national strategy, the population recovery and selection of local pig resources is urgent, and the preservation of frozen semen is an important means. my country has a wealth of endemic species, but now these local species have serious problems of continuous d...

Claims

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Application Information

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IPC IPC(8): A01N1/02A61D19/02
CPCA01N1/021A01N1/0215A01N1/0226A61D19/02
Inventor 翁晓刚牟彦双张宇霆蔡铭铭宋健刘忠华
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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