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A rapid germination medium for primary culture of Chinese fir

A germination medium and primary culture technology, applied in plant regeneration, horticulture, horticultural methods, etc., can solve the problems of restricting the popularization and planting of Chinese fir, limited number of seed strips, and low survival rate of cuttings, etc. Fast speed, good induction effect

Active Publication Date: 2021-09-07
JIANGSU WUJIN HIGHWAY PLANT NURSERY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the main method of propagating Zhongshan fir seedlings is cutting, which can keep the seedlings to maintain the excellent characteristics of the mother tree, but Zhongshan fir seedlings are lignified earlier, the survival rate of cuttings is low, and the number of seedlings is limited, the speed is slow, and the seedling production The rate is low, which restricts the large-scale popularization and planting of Zhongshan fir

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A rapid germination medium for primary culture of Zhongshan fir, including induction medium and strong seedling medium;

[0023] The formula of the induction medium is the 6- The camphor oil of the agar+0.02 organic matter+4g sucrose+2mg / L of BA+10g / L; Wherein said organic matter is the mixture of glutathione reductase and cysteine, and said glutathione reductase The mass ratio to cysteine ​​is 2:1.

[0024] The formula of described strong seedling culture medium is the PVP+10mg of the GA+6mg / L of the ascorbic acid+5mg / L of 1 / 2MS medium+10mg / L sodium nitrophenolate+10mg / L / L of activated carbon powder + 2g / L of sucrose + 10g / L of agar.

Embodiment 2

[0026] A rapid germination medium for primary culture of Zhongshan fir, including induction medium and strong seedling medium;

[0027] The formula of the induction medium is the 6- The camphor oil of the agar+0.06 organic matter+6g sucrose+3mg / L of BA+20g / L; Wherein said organic matter is the mixture of glutathione reductase and cysteine, and said glutathione reductase The mass ratio to cysteine ​​is 2:1.

[0028] The formula of described strong seedling culture medium is the IAA+0.06mg / L of the IAA+0.06mg / L of the ascorbic acid+10mg / L of the ascorbic acid+10mg / L of 1 / 2MS medium+30mg / L sodium nitrophenolate+20mg / L activated carbon powder + 4g / L sucrose + 20g / L agar.

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Abstract

The invention relates to a rapid germination medium for the primary culture of Zhongshan fir, including an induction medium and a strong seedling medium; the medium provided by the invention is rich in nutrients, suitable for the proliferation and rapid germination of Zhongshan fir, and greatly shortens the cultivation time of Zhongshan fir. The cycle reduces the production cost; the germination rate of explants in the first generation of Zhongshan fir is more than 98%, the average germination time is less than 18 days, and the germination rate is fast; it can effectively reduce the browning rate of explants during tissue culture and increase the explant Implant survival rate, improve reproductive efficiency.

Description

technical field [0001] The invention relates to the technical field of Zhongshan fir tissue culture, in particular to a rapid germination medium for primary culture of Zhongshan fir. Background technique [0002] Zhongshan fir is a semi-evergreen tall tree with a tall and straight trunk and a beautiful tree shape. It is an excellent interspecific hybrid of three tree species: North American cypress, Mexican cypress, and pool fir. After years of experimental research by the Institute of Botany, Chinese Academy of Sciences, Jiangsu Province Selected and bred. It has the characteristics of long green period, fast growth, water and humidity resistance, salt and alkali resistance, strong wind resistance, less pests and diseases, wide adaptability, rapid growth, and excellent material quality than its parents. It is not only an excellent tree species for farmland forest network and tidal flat afforestation, but also presents broad development prospects in urban landscaping, water...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 谢健仇玉成许丽群王婷俞佳丽潘海峰俞璟武洁洁沈卿周奕成
Owner JIANGSU WUJIN HIGHWAY PLANT NURSERY
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