A rapid germination medium for primary culture of Chinese fir
A germination medium and primary culture technology, applied in plant regeneration, horticulture, horticultural methods, etc., can solve the problems of restricting the popularization and planting of Chinese fir, limited number of seed strips, and low survival rate of cuttings, etc. Fast speed, good induction effect
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Embodiment 1
[0022] A rapid germination medium for primary culture of Zhongshan fir, including induction medium and strong seedling medium;
[0023] The formula of the induction medium is the 6- The camphor oil of the agar+0.02 organic matter+4g sucrose+2mg / L of BA+10g / L; Wherein said organic matter is the mixture of glutathione reductase and cysteine, and said glutathione reductase The mass ratio to cysteine is 2:1.
[0024] The formula of described strong seedling culture medium is the PVP+10mg of the GA+6mg / L of the ascorbic acid+5mg / L of 1 / 2MS medium+10mg / L sodium nitrophenolate+10mg / L / L of activated carbon powder + 2g / L of sucrose + 10g / L of agar.
Embodiment 2
[0026] A rapid germination medium for primary culture of Zhongshan fir, including induction medium and strong seedling medium;
[0027] The formula of the induction medium is the 6- The camphor oil of the agar+0.06 organic matter+6g sucrose+3mg / L of BA+20g / L; Wherein said organic matter is the mixture of glutathione reductase and cysteine, and said glutathione reductase The mass ratio to cysteine is 2:1.
[0028] The formula of described strong seedling culture medium is the IAA+0.06mg / L of the IAA+0.06mg / L of the ascorbic acid+10mg / L of the ascorbic acid+10mg / L of 1 / 2MS medium+30mg / L sodium nitrophenolate+20mg / L activated carbon powder + 4g / L sucrose + 20g / L agar.
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