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Method for detecting trace Fe<3+>, Hg<2+> and Cu<2+> in living cells through double-channel fluorescence imaging

A fluorescence imaging, dual-channel technology, used in chemical instruments and methods, measurement devices, fluorescence/phosphorescence, etc., can solve the problems of inconvenient detection, poor selectivity, and inability to achieve efficient detection.

Inactive Publication Date: 2018-06-19
GUIZHOU UNIV
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  • Claims
  • Application Information

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Problems solved by technology

[0006] There have been many reports as probes for the detection of trace metal ions in cell imaging, but most of them are single-probe detection of a single target ion, which is inconvenient for detection, poor in selectivity, and cannot achieve efficient detection of multiple target ions in cells by a single probe. question

Method used

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  • Method for detecting trace Fe&lt;3+&gt;, Hg&lt;2+&gt; and Cu&lt;2+&gt; in living cells through double-channel fluorescence imaging
  • Method for detecting trace Fe&lt;3+&gt;, Hg&lt;2+&gt; and Cu&lt;2+&gt; in living cells through double-channel fluorescence imaging
  • Method for detecting trace Fe&lt;3+&gt;, Hg&lt;2+&gt; and Cu&lt;2+&gt; in living cells through double-channel fluorescence imaging

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Embodiment 1

[0057] The synthetic route of the probe:

[0058]

[0059] The specific synthesis method of the probe:

[0060]1) Synthesis of Intermediate 1: In a 100ml three-necked flask, add 27.36mmol of tris(2-aminoethyl)amine, 3.42mmol of rhodamine B and 60ml of absolute ethanol, reflux for 36h under nitrogen protection, and depressurize Ethanol was evaporated, extracted 3 times with 100ml dichloromethane respectively, the organic phase was dried overnight with anhydrous magnesium sulfate, and the solvent was evaporated to obtain a red viscous substance, which was separated by silica gel column chromatography, and the eluent was 9 / 1 / 1 methanol / chloroform / triethylamine to obtain 1.71g of colorless viscous intermediate 1;

[0061] 2) Synthesis of Intermediate 2: In a 250ml three-neck flask, add Intermediate 1, 120ml of chloroform and 5.01mmol of 4-chloro-7-nitrobenzofurazan, react for 2h under nitrogen protection in an ice bath, evaporate Remove the solvent, and separate by silica ge...

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Abstract

The invention discloses a method for detecting trace Fe<3+>, Hg<2+> and Cu<2+> in living cells through double-channel fluorescence imaging. A triamine-ethylamine derivative called as a probe for shortserves as a fluorescence imaging probe for detection of trace Fe<3+>, Hg<2+> and Cu<2+> in living cells through double-channel fluorescence imaging, and the trace Fe<3+>, Hg<2+> and Cu<2+> in livingcells are detected through double-channel fluorescence imaging. By means of the method, detection of multiple target ions can be realized. The probe and Fe<3+>, Hg<2+> and Cu<2+> form a probe-Fe<3+> complex, a probe-Hg<2+> complex and a probe-Cu<2+> complex respectively, strong fluorescence with different wavelengths is emitted, and the method facilitates detection, is good in visibility, high insensitivity and good in selectivity and can realize real-time online detection of Fe<3+>, Hg<2+> and Cu<2+> ions in the cells.

Description

technical field [0001] The invention relates to a method for detecting trace ions in living cells by dual-channel fluorescence imaging, in particular to a method for detecting trace Fe in living cells by dual-channel fluorescence imaging 3+ , Hg 2+ and Cu 2+ Methods. Background technique [0002] In cell biology and medical research, fluorescent probes are the most powerful tools for allowing researchers to monitor events in living cells and living organisms in real time due to their high spatial resolution and minimal disturbance of the living body. One of them is being paid more and more attention to and used. Probes are particularly important for the study of metal ion transport carriers and the crossing behavior of cell membranes in drug delivery systems. Fluorescence imaging to detect intracellular ions can provide the most direct information on the temporal and spatial distribution of ions in living systems. Due to its non-invasive, high sensitivity, and high selec...

Claims

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Application Information

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IPC IPC(8): G01N21/64C07D491/107C09K11/06
CPCC07D491/107C09K11/06C09K2211/1088G01N21/6428G01N21/6486
Inventor 曾晞张红方浚安廖贤牟兰
Owner GUIZHOU UNIV
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