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Method for assaying trace AL3+ in living cells by double-channel fluorescence imaging

A fluorescence imaging, dual-channel technology, applied in fluorescence/phosphorescence, measurement devices, material analysis through optical means, etc., can solve the problems of lack of spectral characteristics, low sensitivity, weak coordination ability, etc., and achieve good coordination ability and selectivity Good, good visibility effect

Inactive Publication Date: 2017-09-29
GUIZHOU UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, compared to other metal ions, Al 3+ The detection of has been difficult due to the lack of spectral features and weak coordination ability
[0004] Therefore, existing methods to detect trace amounts of Al in living cells 3+ , the presence of reagents and Al 3+ Difficult coordination ability, inconvenient detection, poor visibility, low sensitivity, poor selectivity, easy to be interfered by other ions, and cannot be detected online in real time

Method used

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  • Method for assaying trace AL3+ in living cells by double-channel fluorescence imaging
  • Method for assaying trace AL3+ in living cells by double-channel fluorescence imaging
  • Method for assaying trace AL3+ in living cells by double-channel fluorescence imaging

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Embodiment 1

[0037] 1. The synthetic route of the probe:

[0038]

[0039] The specific preparation method of the probe: in a 100ml three-necked flask, add tris(2-aminoethyl)amine (4.0g, 27.36mmol), rhodamine B (1.638g, 3.42mmol), 60ml of absolute ethanol, nitrogen Reflux for 36 h under protection, distill off ethanol under reduced pressure, extract with dichloromethane (3×100ml), dry the organic phase over anhydrous magnesium sulfate overnight, evaporate the solvent, and obtain a red viscous substance, which is separated by silica gel plate chromatography, washed The liquid was removed to methanol / chloroform / triethylamine at a volume ratio of 9 / 1 / 1 to obtain 1.71 g of a colorless viscous intermediate with a yield of 87.3%. The structural characterization data are as follows: 1 H NMR (500MHz, CDCl 3 )δppm 7.89 (d, J=5.0Hz, 1H, ArH), 7.44-7.46 (br, 2H, ArH), 7.10 (bs, 1H, ArH), 6.40~6.42 (m, 4H, ArH), 6.27 (d ,J=5.0Hz,2H,ArH),3.34(q,J=10.0Hz,8H,NCH 2 CH 3 ),3.15(m,2H,NCH 2 CH 2 N)...

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Abstract

The invention discloses a method for assaying trace Al3+ in living cells by double-channel fluorescence imaging, which assays trace Al3+ in living cells by double-channel fluorescence imaging with a probe with the chemical name of tri[di(naphthylthioureido)-rhodamine formamido]ethylamine fluorescence imaging probe for assaying trace Al3+ in the living cells by double-channel fluorescence imaging. In the method disclosed by the invention, the probe and Al3+ are formed into a complex in the cells, strong fluorescence is emitted, consequently, assay is convenient, visuality is good, sensitivity is high, selectivity is good, and the Al3+ ions in the cells can be assayed on line in real time.

Description

technical field [0001] The invention relates to a method for detecting trace ions in living cells by dual-channel fluorescence imaging, in particular to a method for detecting trace amounts of Al in living cells by dual-channel fluorescence imaging 3+ Methods. Background technique [0002] Aluminum is the third most abundant element in the earth's crust. It is widely used in daily life as packaging materials, aluminum foil, container materials, pharmaceutical preparations, bleaching agents, paper industry, food additives, and pharmaceutical packaging. As we all know, aluminum is not an essential element for living organisms, and it will have obvious adverse effects on human health. Among various biologically and environmentally important metal ions, the detection of aluminum is particularly important due to its toxic effects on the environment and human health. Moreover, aluminum-based drugs such as antiperspirants, anti-ulcer drugs, and antacids are more harmful to human ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6486
Inventor 曾晞廖贤方浚安牟兰
Owner GUIZHOU UNIV
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