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Application of raspberry extract in the preparation of drugs for the prevention and treatment of retinal damage diseases

A retinal injury, raspberry technology, applied in the direction of drug combination, sensory disease, cardiovascular system disease, etc., to achieve the effect of strong protective effect, strong protective effect and high drug efficacy

Active Publication Date: 2021-01-01
SHENYANG EYE IND TECH INST LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, which ophthalmic diseases raspberries have specific effects on and the relevant mechanism of action have not been reported at home and abroad.

Method used

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  • Application of raspberry extract in the preparation of drugs for the prevention and treatment of retinal damage diseases
  • Application of raspberry extract in the preparation of drugs for the prevention and treatment of retinal damage diseases
  • Application of raspberry extract in the preparation of drugs for the prevention and treatment of retinal damage diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Raspberry Extract

[0045] (1) Extraction process, such as figure 1

[0046] Take 400g raspberries, grind them into powder, and extract twice with 3200mL and 2400mL of 70% ethanol under reflux, each time for 1.5h, combine the extracts, recover the ethanol under reduced pressure, and obtain 370mL extracts, and transfer the obtained 370mL extracts into a large Carry out chromatographic separation on a porous adsorption resin column, elute with 95% ethanol, and recover the ethanol solvent from the obtained eluate under reduced pressure to obtain 24.49 g ethanol eluate (FPZ-Z).

[0047] The 15.00g ethanol eluate (FPZ-Z) was separated by ODS column chromatography, and eluted with 20% methanol aqueous solution, 40% methanol aqueous solution, 70% methanol aqueous solution and 100% methanol respectively to obtain 20% methanol aqueous solution elution 4.33 g of the product was designated as FPZ-20, 5.66 g of the product eluted with 40% methanol aqueous solution was d...

Embodiment 2

[0057] (1) H 2 o 2 Establishment of induced retinal-associated cell (ARPE-19, RGC5, Muller) damage model

[0058] ARPE-19 cells were subcultured according to 2×10 5 cell / ml drop plate, 100ul / well. After 24h, different concentrations of H 2 o 2 (0mM, 0.5mM, 1mM, 1.5mM, 2mM) acted on ARPE-19 cells for 24h respectively. Coloring: Add 20ul of MTT solution to each well and continue to incubate for 2-4h. Colorimetry: Select a wavelength of 490nm, measure the light absorption value of each well on an enzyme-linked immunosorbent monitor, record the results, and use different concentrations of H 2 o 2 The cell growth curve is drawn as the abscissa and the absorbance value as the ordinate. When the cell viability was around 50%, the H 2 o 2 The concentration was used as the modeling concentration of the oxidative damage model.

[0059] Figure 3A different concentrations of hydrogen peroxide (H 2 o 2 ) to establish the ARPE-19 cell model. Depend on Figure 3A It can be s...

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Abstract

The invention discloses a use of raspberry extract in preparation of drugs for preventing and treating retina injury diseases. The protection effect of raspberry extract on the H2O2-induced oxidativestress damage to human retina related cells (ARPE-19, RGC5 and Muller) is observed and raspberry effective active monomers with effects of protecting damaged retinal pigment epithelium (RPE) are screened. The use provides the theoretical basis for prevention and treatment on retinal cell injury associated eye diseases through the raspberry extract and related component monomers.

Description

technical field [0001] The present invention relates to a new pharmacological action of a natural plant traditional Chinese medicine, in particular to the application of raspberry and its active component monomers in the preparation of health care products or new medicines for the prevention and treatment of retinal damage diseases, and is useful for the prevention and treatment of ophthalmology-related diseases. Clinical treatment develops new methods and ideas. Background technique [0002] The retina, also known as the peripheral brain, is the same as the brain in origin and is the part that has direct contact with the outside world. The sensory layer on the retina is composed of three neurons, whose main function is to transmit visual information to the visual center step by step to form vision and be responsible for visual transmission. The types of cells included in the retina are: pigment epithelial cells, cone cells, rod cells, biganglion cells, ganglion cells and M...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/73A61K45/06A61K31/352A61K31/7048A61P27/02A61P9/10
CPCA61K31/352A61K31/7048A61K36/73A61K45/06A61K2300/00
Inventor 张东蕾杜威威何向东何伟
Owner SHENYANG EYE IND TECH INST LTD
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