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A sugarcane endophytic Burkholderia cz08152 and its application

A technology for growing Burkholderia and Burkholderia, which is applied to endophytic Burkholderia CZ08152 in sugarcane and its application field, and can solve the problem of poor soil water retention, poor fertilizer retention, and fertilizer problems. Problems such as low utilization rate can achieve the effects of promoting crop growth, protecting the ecological environment, and enhancing resistance

Active Publication Date: 2021-09-03
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, more than 90% of the sugarcane land in my country's main sugarcane production areas is dry slope land, the soil water and fertilizer retention capacity is poor, and the utilization rate of chemical fertilizers is very low.

Method used

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  • A sugarcane endophytic Burkholderia cz08152 and its application
  • A sugarcane endophytic Burkholderia cz08152 and its application
  • A sugarcane endophytic Burkholderia cz08152 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Isolation, screening and identification of strains

[0029] First, the separation of strains

[0030] Test materials: collected from the Guangxi Zhuang Autonomous Region Chongzuo sugarcane varieties grown in the outskirts of ROC tissue stem base number of 22.

[0031] Sugar cane take in clean table first with 70% ethanol immersion 30s, rinsed three times with sterile water, and then soaked in 0.1% mercuric chloride for 1min, 70% ethanol immersion 30s, sterile water five times. Weigh 1g tissue surface sterilized phosphate buffer 10mL block immersed in a sterile mortar after standing was triturated 5min, supernatant taken 10 fold dilution made with sterile water and dilutions of stock solution were taken 100μL Ashby nitrogen-free coating medium plates 2-3d culture grown to single colonies at 28 ℃. After colonies were grown in bacteria and purified by Ashby no scribing method performed on a plate medium nitrogen.

[0032] Second, confirm the sugarcane strain azotobacteria

[0...

Embodiment 2

[0060] Berkholdia CZ08152 strain to promote growth effect on sugarcane

[0061] The cultivated matrix is ​​a normal straw seedlings, and 10 mg is added per kg substrate ( 15 NH 4 ) 2 SO 4 , Dispensed into a plastic basin. After the activation of Berkhobacterium CZ08152, it was cultured overnight in LB liquid medium, centrifugally collected bacteria, and centrifugally washed with sterile water and washed twice. 1-2 × 10 per milliliter 1-2 × 10 8 Cells, i.e., CZ08152, CZ08152, vaccinated. The group culture seedlings of sugarcane variety (ROC22) were selected as inoculated objects, and the sugarcane group culture seedlings consistent with the seedling seedlings were planted into a plastic basin equipped with a cultivated matrix. The resulting Berkho Erdi CZ08152 vaccinated 100ml, as an experimental group "inoculated CZ08152". The inoculation isometric sterile water, the inoculation of Berk Holdacteria CZ08152 is replaced with an isometric sterile water, and the remaining operation is...

Embodiment 3

[0066] The promotion of the growth of corn seedlings in Berkholdia CZ08152

[0067] After activation of Berk Holds CZ08152, cultured overnight in LB liquid medium, centrifugally collected a bacteria, and centrifugally, with a sterile water, centrifugation was cleaned 2 times, diluted to 1-2 × 10 per milliliter 1-2 × 10 8 A cell is used as a biochemical suspension, that is, CZ08152 in Berk Holder CZ08152. Take a small size of corn seeds for 30 min in 2% NaClo, washed with sterile water to no NaClo residue, placed in aseptic petri dish, soaked in a sterile petri dish, immersed the antibody 1d, pour the excess water, then placed in 30 Grouting at ° C. After the seed is gown, it is broadcast in the normal straw seedlings. When the second sheet is completely expanded, the root is completely expanded and transplanted (the specific method is: select the size of the seedlings to be placed in Berk Holds CZ08152 bacterial suspension As the experimental group "vaccination CZ08152"; the contr...

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Abstract

The invention discloses a strain of endophytic Burkholderia sp. CZ08152 in sugarcane. The classification of Burkholderia sp. CZ08152 is named as Burkholderia sp. CZ08152. The list is as shown in SEQ ID NO.1, and the depository unit is: General Microorganism Center of China Committee for Culture Collection of Microorganisms, and the deposit number is CGMCC No.5743. The invention can be applied to the production of microbial agents and bio-organic fertilizers that can promote the growth of crops, inhibit plant diseases, and improve plant resistance to adversity stress, enhance the resistance of plants to adversity stress, and play an important role in reducing the use of chemical fertilizers and pesticides and protecting the ecological environment. significance and has broad application prospects.

Description

Technical field [0001] The present invention relates to the field of biotechnology, and more particularly to a cane, Burk Holder, CZ08152, and its application thereof. Background technique [0002] Guangxi is my country's largest sucrose production area, sugar cane and sucrose production account for more than 60% of the country. However, the cost of sugar cane in my country has always been high, lacking international competitiveness. Among the various agricultural materials in sugar cane, fertilizers, especially the proportion of nitrogen fertilizer, and its effect on sugar cane production is also the most significant. In addition, 90% of the sugar cane, soil watering, poor storage capacity, poor fertilizer utilization. Sugarcane tip rot is a commonly occurring sugarcane disease, and the fryarium moniliforme sheldon has the occurrence of the disease in my country's sugarcane area, Guangdong, Fujian, Yunnan. The main pathogenesis of sugar cane tidal rot in the tip of sugar cane, t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P3/00A01P21/00C12R1/01
CPCA01N63/00C12N1/205C12R2001/01
Inventor 胡春锦曾泉史国英农泽梅岑贞陆叶雪莲
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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