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Acid-proof trichoderma koningiopsis and application thereof for inhibiting rhizoctonia solani

A technology for simulating Trichoderma corning and mycelium is applied in the field of microorganisms, which can solve problems such as the use of Trichoderma, and achieve the effects of strong acid resistance, improving agricultural production environment, and maintaining the balance of farmland ecosystems.

Active Publication Date: 2018-06-12
MOON (GUANGZHOU) BIOTECH CO LTD
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Problems solved by technology

[0010] Trichoderma plays an important role in the control of Rhizoctonia solani, but there are few reports on the acid resistance of Trichoderma, which limits the use of Trichoderma in acidified environments. Many studies have shown that in acidic conditions The incidence of plant soil-borne diseases is higher than that under neutral conditions, so it is of great significance to screen acid-tolerant Trichoderma and study its inhibitory effect on pathogenic bacteria under acidic conditions

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  • Acid-proof trichoderma koningiopsis and application thereof for inhibiting rhizoctonia solani
  • Acid-proof trichoderma koningiopsis and application thereof for inhibiting rhizoctonia solani
  • Acid-proof trichoderma koningiopsis and application thereof for inhibiting rhizoctonia solani

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Embodiment Construction

[0038] The present invention will be specifically introduced below in conjunction with the accompanying drawings and specific embodiments.

[0039] Part 1: Isolation and Screening to Obtain Acid-resistant Trichoderma pseudokoningeni

[0040] 1. Medium

[0041] Trichoderma selective solid medium: MgSO 4 ·7H 2 O 0.2g / L, K 2 HPO 4 0.9g / L, KCl 0.15g / L, NH 4 NO 3 1.0g / L, glucose 3.0g / L, chloramphenicol 0.25g / L, sulfonate 0.3g / L, pentachloronitrobenzene 0.2g / L, rose bengal 0.15g / L, agar 15g / L, mixed After homogeneity, sterilize at 120°C for 20 minutes, pack it separately when the temperature drops to 50°C-60°C, and let it cool and dry for later use.

[0042] Acid-resistant Trichoderma selective solid medium: Add 2.5 mg of hydrochloric acid with a concentration of 20% to each liter of PDA medium to obtain an acid-resistant Trichoderma selective solid medium with pH 3.0, sterilize at 120°C for 20min, and the temperature drops to When the temperature is 50℃~60℃, put it on a 90m...

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Abstract

The invention discloses an acid-proof trichoderma koningiopsis and application thereof for inhibiting rhizoctonia solani. The acid-proof trichoderma koningiopsis is classified and named as the trichoderma koningiopsis, and is preserved in the China General Microbiological Culture Collection Center (CGMCC) with a preservation number of CGMCC NO.14878. The acid-proof trichoderma koningiopsis has thefollowing beneficial effects: (1) the acid-proof trichoderma koningiopsis has better acid resistance and can tolerate acidification of which the pH is 3.0 or above, after the acid-proof trichoderma koningiopsis is introduced into acidified soil, the rhizoctonia solani can be effectively prevented and treated through biological control, the agricultural production environment can be effectively improved, and the ecological system of a farmland is kept balanced; (2) when the acid-proof trichoderma koningiopsis is used in the farmland, the growth of the rhizoctonia solani can be inhibited; (3) the acid-proof trichoderma koningiopsis has a good inhibition function for various soil-borne disease pathogenic bacteria in both acidic conditions and neutral conditions, the acid-proof trichoderma koningiopsis can prevent and treat target bacterial strains after being applied to soil, various soil-borne diseases can be prevented, and the stability of a soil microbial community is maintained.

Description

technical field [0001] The invention relates to a strain of mold and its application, in particular to an acid-resistant Trichoderma pseudokonine and its application in inhibiting Rhizoctonia solani, belonging to the technical field of microorganisms. Background technique [0002] Rhizoctonia solani (Rhizoctonia solani) is widely distributed in nature, has a wide range of hosts and strong saprophytic properties, and can cause various diseases of vegetables, fruits and crops. It mainly causes root rot, seed rot, damping-off and blight of plants, and also causes sheath blight of cereal crops, and can also infect corn, soybeans, peanuts, sugarcane, cotton, etc. The fungus usually lives in the soil in the form of hyphae and sclerotia, and survives in the soil for a long time. Continuous cropping and crop rotation can cause a large amount of pathogenic bacteria to accumulate, and the disease becomes serious in the middle and late stages of seedling cultivation, causing a large nu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
CPCA01N63/30C12N1/145C12R2001/885
Inventor 金漫蒋先芝王科晶
Owner MOON (GUANGZHOU) BIOTECH CO LTD
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