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Preparation method of nano silver bactericide for inhibiting bipolaris maydis

Inactive Publication Date: 2018-06-08
ANHUI SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the long-term, large-scale, and single use of chemical pesticides, pathogenic bacteria gradually develop drug resistance. As of now, only for benzimidazole fungicides, nearly 60 genera and more than 100 pathogenic bacteria have produced it in the world. apparent drug resistance

Method used

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  • Preparation method of nano silver bactericide for inhibiting bipolaris maydis
  • Preparation method of nano silver bactericide for inhibiting bipolaris maydis
  • Preparation method of nano silver bactericide for inhibiting bipolaris maydis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] A kind of preparation method of the nano-silver bactericide that suppresses corn spot bacterium, comprises the following steps:

[0045] (1) Remove the core of ginkgo, wash and dry;

[0046](2) Take 10g of ginkgo pulp into a beaker, add 100ml of deionized water and mix evenly, then place it in a water bath at 95°C and heat for 30min, during the heating process, keep stirring with a glass rod to form a mixture;

[0047] (3) Filter the mixture while it is hot in a funnel lined with sterile filter paper, and cool the filtrate to room temperature for later use;

[0048] (4) Use a pipette gun to absorb 5ml of filtrate and dilute to 100ml with deionized water to make the first mixed solution;

[0049] (5) Add AgNO to the first mixture 3 powder, so that AgNO in the mixed solution 3 Concentration is 1mmol / L, obtains the second mixed liquor;

[0050] (6) adding HCl solution and NaOH solution to the second mixed solution to make the pH value of the second mixed solution 3 to ...

Embodiment 2

[0056] A kind of preparation method of the nano-silver bactericide that suppresses corn spot bacterium, comprises the following steps:

[0057] (1) Remove the core of ginkgo, wash and dry;

[0058] (2) Take 10g of ginkgo pulp into a beaker, add 100ml of deionized water and mix evenly, then place it in a water bath at 95°C and heat for 30min, during the heating process, keep stirring with a glass rod to form a mixture;

[0059] (3) Filter the mixture while it is hot in a funnel lined with sterile filter paper, and cool the filtrate to room temperature for later use;

[0060] (4) Use a pipette gun to absorb 20ml of the filtrate and dilute to 100ml with deionized water to make the first mixed solution;

[0061] (5) Add AgNO to the first mixture 3 powder, so that AgNO in the mixed solution 3 Concentration is 5mmol / L, obtains the second mixed solution;

[0062] (6) adding HCl solution and NaOH solution to the second mixed solution to make the pH value of the second mixed soluti...

Embodiment 3

[0068] A kind of preparation method of the nano-silver bactericide that suppresses corn spot bacterium, comprises the following steps:

[0069] (1) Remove the core of ginkgo, wash and dry;

[0070] (2) Take 10g of ginkgo pulp into a beaker, add 100ml of deionized water and mix evenly, then place it in a water bath at 95°C and heat for 30min, during the heating process, keep stirring with a glass rod to form a mixture;

[0071] (3) Filter the mixture while it is hot in a funnel lined with sterile filter paper, and cool the filtrate to room temperature for later use;

[0072] (4) Use a pipette gun to absorb 50ml of filtrate and dilute to 100ml with deionized water to make the first mixed solution;

[0073] (5) Add AgNO to the first mixture 3 powder, so that AgNO in the mixed solution 3 Concentration is 8mmol / L, obtains the second mixed solution;

[0074] (6) adding HCl solution and NaOH solution to the second mixed solution to make the pH value of the second mixed solution 1...

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Abstract

The invention provides a preparation method of a nano silver bactericide for inhibiting bipolaris maydis. The preparation method comprises the following steps: heating 10 parts by weight of gingko pulp and 100 parts by weight of deionized water in a water bath kettle at 95 DEG C for 30min, and carrying out filtering while the mixture is hot; mixing the filtrate with deionized water according to avolume ratio of 1:1-19; adding AgNO3 to make the concentration of AgNO3 in the mixed solution be 1-8mmol / L, adding HCl and NaOH to make the pH value be 3-11, putting the mixture in a constant temperature environment of 4-80 DEG C for reaction for 15min, filtering the product twice with a microporous filter membrane with pores of 0.22[mu]m to obtain a filtrate, centrifuging the filtrate at 12000rpmfor 20min, discarding the supernatant, adding anhydrous ethanol, centrifuging the mixture again, discarding the supernatant, and finally carrying out drying at 65 DEG C to obtain nano silver powder;and preparing the nano silver powder into 10mg / ml nano silver mother liquor which is mixed with a 10mg / ml bactericide to obtain the nano silver bactericide.

Description

technical field [0001] The invention relates to a method for inhibiting plant pathogenic fungi, in particular to a preparation method for a nano-silver fungicide for inhibiting corn leaf spot fungus. Background technique [0002] Maize spot disease is one of the important diseases that commonly occur in maize producing areas in the world. In our country, especially the summer corn producing areas suffer the most. Generally, it results in a 15%-20% reduction in production, and in severe cases, it can reach more than 50% or even no grains. Although there are various control methods for plant diseases, chemical control is still the main measure to control maize spot disease. However, with the long-term, large-scale, and single use of chemical pesticides, pathogenic bacteria gradually develop drug resistance. As of now, only for benzimidazole fungicides, nearly 60 genera and more than 100 pathogenic bacteria have produced it in the world. obvious drug resistance. In addition...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N59/16A01N61/00A01N43/653A01N47/14A01N43/36A01P3/00B22F9/24B82Y40/00
CPCA01N43/36A01N43/653A01N47/14A01N59/16A01N61/00B22F9/24B82Y40/00
Inventor 黄伟东兰彬段海明武德功杜军利余海兵
Owner ANHUI SCI & TECH UNIV
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