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Method for fermenting and producing curdlan

A fermentation liquid and fermentation culture technology, applied in the field of fermentation and production of curdlan gum, can solve the problems of low yield, long fermentation cycle, complicated process operation, etc., and achieve the effects of increased yield, simple operation and low price

Inactive Publication Date: 2018-06-01
潍坊市海奥斯生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is: in view of the problems of long fermentation period, complicated process operation and low yield in the current production, the present invention provides a method for fermenting curdlan gum: by adding non-ionic surfactant and Application of feeding and other fermentation technologies can significantly shorten the fermentation period and increase the yield of curdlan gum

Method used

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  • Method for fermenting and producing curdlan
  • Method for fermenting and producing curdlan
  • Method for fermenting and producing curdlan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] a. Seed culture: After the Agrobacterium strains are activated, they are transferred to the seed culture solution for cultivation. The cultivation temperature is 28-32°C, the pH value is adjusted to 6.5-7.5, the rotation speed is 180-200rpm, and the cultivation time is 28-28°C. 32h; wherein the components of the seed culture solution are: glucose 20g / L, corn steep liquor dry powder 10g / L, K 2 HPO 4 2g / L, MgSO 4 1g / L.

[0033] B. the grown Agrobacterium seed liquid is inserted in the 50L fermenter that 30L fermentation medium is housed with the inoculum size of 5% (v / v), and initial fermentation medium formula is: glucose 60g / L, dry powder of corn steep liquor 4g / L, (NH 4 ) 2 HPO 4 3g / L, KH 2 PO 4 2g / L, MgSO 4 1g / L, FeCl 3 0.01g / L, ZnCl 2 0.01g / L, polyether defoamer 0.04g / L, pH 6.8-7.0. The culture temperature is 30°C, the tank pressure is 0.05MPa, the ventilation ratio is 0.5vvm, and the stirring speed is 350r / min.

[0034] In the fermentation process...

Embodiment 2

[0040] a. Seed culture: After the Agrobacterium strains are activated, they are transferred to the seed culture solution for cultivation. The cultivation temperature is 28-32°C, the pH value is adjusted to 6.5-7.5, the rotation speed is 180-200rpm, and the cultivation time is 28-28°C. 32h; wherein the components of the seed culture solution are: glucose 20g / L, corn steep liquor dry powder 10g / L, K 2 HPO 4 2g / L, MgSO 4 1g / L.

[0041]B. the grown Agrobacterium seed liquid is inserted in the 50L fermenter that 30L fermentation medium is housed with the inoculum size of 5% (v / v), and initial fermentation medium formula is: glucose 60g / L, dry powder of corn steep liquor 4g / L, (NH 4 ) 2 HPO 4 3g / L, KH 2 PO 4 2g / L, MgSO 4 1g / L, FeCl 3 0.01g / L, ZnCl 2 0.01g / L, polyether defoamer 0.04g / L, pH 6.8-7.0. The culture temperature is 30°C, the tank pressure is 0.05MPa, the ventilation ratio is 0.5vvm, and the stirring speed is 350r / min.

[0042] During the fermentation proc...

Embodiment 3

[0048] a. Seed culture: After the Agrobacterium strains are activated, they are transferred to the seed culture solution for cultivation. The cultivation temperature is 28-32°C, the pH value is adjusted to 6.5-7.5, the rotation speed is 180-200rpm, and the cultivation time is 28-28°C. 32h; wherein the components of the seed culture solution are: glucose 20g / L, corn steep liquor dry powder 10g / L, K 2 HPO 4 2g / L, MgSO 4 1g / L.

[0049] B. the grown Agrobacterium seed liquid is inserted in the 50L fermenter that 30L fermentation medium is housed with the inoculum size of 5% (v / v), and initial fermentation medium formula is: glucose 60g / L, dry powder of corn steep liquor 4g / L, (NH 4 ) 2 HPO 4 3g / L, KH 2 PO 4 2g / L, MgSO 4 1g / L, FeCl 3 0.01g / L, ZnCl 2 0.01g / L, polyether defoamer 0.04g / L, pH 6.8-7.0. The culture temperature is 30°C, the tank pressure is 0.05MPa, the ventilation ratio is 0.5vvm, and the stirring speed is 350r / min;

[0050] Feed KOH during the ferment...

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Abstract

The invention discloses a method for fermenting and producing curdlan. After activation of culture, the product of curdlan is obtained through fine extraction after seed cultivation and fermented cultivation, wherein the control for fermented cultivation conditions is carried out by adopting the technologies of controlling the pH value, supplementing carbon sources in multiple times and adding a non-ionic surface active agent during fermented cultivation. The method solves the problems of long fermentation period, complex technological operation, low yield and the like in current production; through technical control, the fermentation period can be shortened obviously, and the yield of the curdlan is improved.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation engineering, and in particular relates to a method for producing curdlan by fermentation. Background technique [0002] Curdlan gum (Curdlan) is a microbial exopolysaccharide produced by fermentation of Agrobacterium sp. or Rhizobium sp. After the source is exhausted, curdlan gum is secreted, which surrounds the bacteria and is insoluble in water. Curdlan gum has the characteristics of forming a reversible gel under low temperature heating conditions and an irreversible gel under high temperature conditions, and is widely used in the food industry as a food additive. It can improve the water retention and stability of the product, and increase the hardness and taste of the product. In addition, it also has good applications in fields such as oil extraction. [0003] Non-ionic surfactants have hydrophilic groups and lipophilic groups, adding a small amount to the solution can sig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/01
CPCC12P19/04
Inventor 赵忠胜赵文国崔刚
Owner 潍坊市海奥斯生物科技有限公司
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