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Preparation method of gradient bionic artificial vitreous body

A technology of vitreous body and preparation process, applied in prosthesis, tissue regeneration, medical science and other directions, can solve problems such as easy degradation, and achieve the effect of good biocompatibility, excellent biocompatibility and long maintenance time

Active Publication Date: 2018-06-01
SHANGHAI QISHENG BIOLOGICAL PREPARATION CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, biomacromolecules are slightly cross-linked with cross-linking agents, which can effectively prolong their degradation time in vivo and solve the problem that ordinary biomacromolecules are easily degraded in the eye

Method used

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  • Preparation method of gradient bionic artificial vitreous body
  • Preparation method of gradient bionic artificial vitreous body

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Preparation of HA Nanospheres with Cystamine Grafted on the Surface in Phosphate Buffer

[0061] At 4°C, add 0.01g HA (Mw=1×10 6 ) was dissolved in 1 mL of PBS (pH=7.2) containing BDDE (1% wt). After the dissolution was complete, it was added to a 250 mL three-necked flask containing 80 mL of n-hexane (containing 0.05% wtSDS). The addition rate was 0.03 mL / min, while the system was mechanically stirred at a speed of 1000 rpm, and the reaction was maintained at 30°C for 4 hours, and then at 60°C for 1 hour. Then, the reaction system was subjected to suction filtration (0.45 μm filter membrane), and the collected HA nanospheres were washed with 100 mL of PBS for 3 times, each time for 30 minutes. Microspheres were stored in PBS.

[0062] Weigh 0.1g HA nanospheres and add them into 10mL PBS solution containing cystamine (10%wt), then add 0.25g DMTMM under the condition of mechanical stirring (300rpm), react for 6 hours, suction filter, and wash with 50mL PBS Wash 3 tim...

Embodiment 2

[0064] Preparation of gelatin system with sulfhydryl groups in phosphate buffer (alcohol precipitation method)

[0065] Prepare 100mL gelatin / PBS solution of 1% wt at 40°C, add 2.65g citric acid, add 1.00g DMTMM after dissolving evenly, mechanically stir at 200rpm for 24 hours, settle with ethanol, and wash three times. The alcohol-precipitated powder was dried in a vacuum drying oven to constant weight, and finally a gelatin powder joined with citric acid was obtained.

[0066] The above powder was prepared into 1%wt 50mL PBS solution at 40°C, 0.84g (2 times the mole number) of cysteine ​​was added, and 0.19%wt of DMTMM was added after it was dissolved evenly. After stirring and dissolving, let stand for 24h. Settled again with ethanol and washed three times. The obtained alcohol-precipitated powder was dried to constant weight in a vacuum oven, and the 2 environment. When in use, it is redissolved with PBS solution to obtain a gelatin system with sulfhydryl groups.

Embodiment 3

[0068] Preparation of gelatin system with sulfhydryl groups in phosphate buffer (dialysis method)

[0069] Prepare 100 mL of 1% wt gelatin / PBS solution at 40°C, add 3.20 g of oxalic acid, add 1.00 g of DMTMM after it is uniformly dissolved, and mechanically stir at 200 rpm for 24 hours. The reacted system was put into a dialysis bag (molecular weight cut-off 10,000Da) for dialysis against PBS, and the solution was changed every day for 4 days. The end result is gelatin bound with citric acid.

[0070] Take 50 mL of gelatin solution connected with citric acid, add 0.84 g (2 times the number of moles) of cysteine, dissolve evenly, and then add 0.19% wt of DMTMM. After stirring and dissolving, let stand for 16h. The reacted system was packed into a dialysis bag (molecular weight cut-off 10,000Da) in N 2 The environment was dialyzed with PBS, and the medium was changed every day for 4 days. Finally, a gelatin system with mercapto groups is obtained.

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Abstract

The invention develops a hybrid cross-linked gel used for substituting vitreous body. Sodium hyaluronate nano-microsphere is covered by a biomacromolecule network, and the sodium hyaluronate concentration is in gradient distribution, thereby simulating the structure and composition change of the human vitreous body, wherein the sodium hyaluronate nano-microsphere is in light cross-linking, can form a reversible cross-linking network with the biomacromolecule, has a certain self-healing capacity, and is capable of effectively proloning the maintenance time in the body; and meanwhile, the compositions of the hybrid cross-linked gel are all the biomacromolecule and has good biocompatibility.

Description

technical field [0001] The invention relates to the technical field of medical biomaterials. Two different systems are mixed to prepare a soft gel containing nano-microspheres with a gradient change in concentration, which can be used as a substitute for human vitreous body to treat retinal fissures and retinal detachment and other symptoms. Background technique [0002] The vitreous humor is a gel structure that fills between the lens and the retina. It is the largest tissue in the eye and plays an important role in the growth and structure of the eye. More than 95% of the specific gravity in the vitreous body is water, and 15-20% of it exists in the form of combined water with mucopolysaccharides, which has the effect of ensuring the stable structure of the vitreous body. Although the protein content in the vitreous is very small, it is diverse and complex. Another important substance in the vitreous is mucopolysaccharide, the main components of which are hyaluronic acid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/48A61L27/52A61L27/58
CPCA61L27/48A61L27/52A61L27/58A61L2430/16
Inventor 不公告发明人
Owner SHANGHAI QISHENG BIOLOGICAL PREPARATION CO LTD
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