Microinjection operation method

A technology of microinjection and injection needle, which can be applied in the direction of using microinjection method, other methods of inserting foreign genetic materials, recombinant DNA technology, etc., and can solve problems such as inability to correspond

Active Publication Date: 2018-05-25
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the above-mentioned problem that the needle tip of the injection needle pulled out by the needle pulling instrument cannot be used to control the inner diameter of the broken needle mouth, the present invention provides a method that can be conveniently adjusted when the injection needle breaks by adjusting the position of the injection needle and the angle of the fixed needle. A convenient and quick method of microinjection operation to quickly obtain a suitable size of needle port

Method used

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Embodiment Construction

[0023] During the microinjection operation, the most critical step is the broken needle of the injection needle. If the fracture of the needle tip of the injection needle is too thick, the death rate of the cells during injection will increase; if the fracture is too thin, the injection volume will be too small and the needle will be easily blocked. The general way to break an injection needle is that the needle hits the wall of the fixed needle tube to break the needle tip of the injection needle, but the size of the needle opening after the needle is broken is random, and the inner diameter of the needle opening after the needle is broken is 3-5 μm. Appropriate, so this operation requires a certain technical content and the operation time is generally long and accidental. This method also has high requirements on the injection needle, the needle point of the injection needle must be very sharp and short (5-8mm) and must have a certain hardness. When the needle pulling instr...

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Abstract

The invention discloses a microinjection operation method. In the microinjection operation method, a needle pulling instrument, a fixing needle, an injection needle, a fixing needle operating arm, aninjection needle operating arm and a microscope are used at least, wherein the fixing needle is rotationally connected with the fixing needle operating arm; the injection needle is rotationally connected with the injection needle operating arm; the microscope comprises an object stage; a cell carrier is arranged on the object stage; a first knob is arranged on the fixing needle operating arm; a second knob is arranged on the injection needle operating arm; the fixing needle has the bending angle of 30 degrees. The invention provides the convenient and rapid microinjection operation method, bywhich when the injection needle breaks, a needle mouth with the suitable size can be conveniently and rapidly obtained through adjustment of the position of the injection needle and the angle of the fixing needle.

Description

technical field [0001] The invention relates to a microinjection operation, in particular to a microinjection operation method. Background technique [0002] Microinjection technology is a technology that injects exogenous genes or other substances into cells by using microinjection needles after adjusting and fixing the injected cells in the field of view of the microscope. It is one of the important technical means of modern bioengineering, especially transgenic , cloning and test-tube babies and other cell engineering fields. Compared with other transgenic techniques, microinjection has the advantages of stable transgenic efficiency, unlimited injection gene size, no need for vectors, and no special requirements for operating objects, etc. been widely used in. [0003] During the microinjection operation, the most critical step is the broken needle of the injection needle. If the fracture of the needle tip of the injection needle is too thick, the death rate of the cel...

Claims

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Application Information

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IPC IPC(8): C12N15/89
CPCC12N15/89
Inventor 代小新高阳赵波牟毅储张杰蔡雅松彭莹莹张政霆张林
Owner ZHEJIANG OCEAN UNIV
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