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Lytic vibrio phage ValDsh-1, sterilization composition containing phage and application of lytic vibrio phage ValDsh-1 and sterilization composition

A valdsh-1 and bacteriophage technology, applied in the biological field, can solve the problems of narrow lysed host spectrum, short duration of lysed ability, easy resistance of host bacteria, etc., and achieve good control effect, low production cost, and high potency Effect

Inactive Publication Date: 2018-05-25
SHENZHEN INST OF ADVANCED TECH
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

As one of the important weapons to deal with antibiotic resistance, phage has great potential for clinical application, but as far as its current application of single phage therapy is concerned, there are still some potential disadvantages in the application process, such as narrow lytic host spectrum and lytic ability. The duration is short, and the host bacteria are easy to develop resistance, etc. Among them, the host bacteria are easy to develop resistance as the main defect in the application of single phage

Method used

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  • Lytic vibrio phage ValDsh-1, sterilization composition containing phage and application of lytic vibrio phage ValDsh-1 and sterilization composition
  • Lytic vibrio phage ValDsh-1, sterilization composition containing phage and application of lytic vibrio phage ValDsh-1 and sterilization composition
  • Lytic vibrio phage ValDsh-1, sterilization composition containing phage and application of lytic vibrio phage ValDsh-1 and sterilization composition

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Embodiment

[0093] The present invention separates and obtains 7 strains of pathogenic Vibrio from its lesions by collecting environmentally friendly fish and shrimp samples in the water samples of Shanwei prawn breeding grounds. The screening flow chart of the bacteriophage of the present invention is as follows figure 1 shown. The pathogenicity of the isolated Vibrio was confirmed through field experiments, and 5 of them were identified as highly pathogenic, LD 50 4 CFU / mL, action time <100h.

[0094] By plate spot method, according to the presence or absence of transparent spots, the host spectrum of the Vibrio phage under 32 strains of different species was determined (Table 1), and it was concluded that the phage had strong host specificity and was a Vibrio phage. A specific split phage.

[0095] Table 1 host spectrum

[0096]

[0097]

[0098] Remarks: "+" means it can be lysed, "-" means it can't be lysed; the above strains are all isolated from aquaculture fish and shrimp...

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Abstract

The invention relates to lytic vibrio phage ValDsh-1, a sterilization composition containing the phage and application of the lytic vibrio phage ValDsh-1 and the sterilization composition. The phage can fast inhibit the growth of vibrio within a short time; in addition, the inhibition effect can be maintained for a long time. In addition, the sterilization composition of the phage is safe, effective, high in specificity and is low in production cost; in the pathogen control process, the defects of a single phage treatment method are overcome.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a Vibrio schisis phage ValDsh-1 and a bactericidal composition containing the phage and its application. Background technique [0002] my country is a big country in aquaculture. In recent years, my country's aquaculture industry has developed rapidly. With the increasing scale of marine aquaculture and the continuous promotion of intensive farming methods, the frequent occurrence of fish diseases poses a serious threat to the healthy development of large-scale marine fish farming. Fish pathogens mainly include bacteria, viruses, parasites, etc. Among them, bacterial diseases are a type of disease that is more harmful to fish. [0003] Vibrio is a short rod-shaped spore-shaped bacterium that can move. It is widely distributed in seawater and organisms in coastal and estuary sea areas. It is one of the most common bacterial groups in the ocean, and it is also the most important path...

Claims

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Application Information

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IPC IPC(8): C12N7/00A61K35/76A61P31/04C12R1/92
CPCA61K35/76C12N7/00C12N2795/00021C12N2795/00032A61K2300/00Y02A50/30
Inventor 马迎飞樊继强王婧婷陈玲刘陈立
Owner SHENZHEN INST OF ADVANCED TECH
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