Method for fast screening different producing area difference of barbat skullcap via UPLC-MS-MS

Abstract

The invention discloses a method for fast screening the different producing area difference of barbat skullcap via UPLC-MS-MS. The method comprises: (1) collecting barbat skullcap medicinal materialsfrom different producing areas in different batches to prepare standard decoction samples; (2) allowing the samples to enter a UPLC-MS-MS system for analysis to obtain corresponding mass spectrum data; (3) directly importing originally collected data to Compound Discoverer 2.1 for data analysis; (4) adopting a dynamic programming calculation method to correct drift of chromatographic peaks, aligning the chromatographic peaks, and registering a substance with the approximate retention time as a compound by using a nearest neighbor clustering method; and (5) analyzing and computing difference substances among samples by using variance, and determining the difference of barbat skullcap obtained from different producing areas. According to the method, compound mass spectrum information is output as an original file, which doesn't need to be converted to other formats and can be directly imported into Compound Discoverer 2.1 database for compound search. The method allows different compounds in the barbat skullcap samples to be fast locked and is suitable for fast analyzing and measuring massive samples.

Description

technical field [0001] The invention belongs to the technical field of research on components of traditional Chinese medicines, and in particular relates to a UPLC-MS-MS method for quickly screening the differences of different producing areas of Scutellaria barbata. Background technique [0002] Banzhilian Scutellaria barbata The dried whole herb of D.Don is mainly produced in Hebei, Henan, Shanxi, Shaanxi, Anhui and other places. The active ingredients of Chinese medicinal materials are different due to factors such as regions, climates, varieties, etc. Even if the same Chinese medicinal materials are used, there will be differences in active ingredients. Therefore, screening the same medicinal materials from different origins has important practical significance for improving the quality of traditional Chinese medicines. [0003] At present, the identification methods for the origin of Chinese medicinal materials mainly include HPLC, IR, NIR, TLC, etc. There are a larg...

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Problems solved by technology

[0003] At present, the identification methods for the origin of Chinese medicinal materials mainly include HPLC, IR, NIR, TLC, etc. There are a large number of false positives and false negatives in these methods, resulting in the possibility of missing important material information when using these methods for analysis, and then As a result, the screened differential substances cannot effectively characterize the group differences, which brings difficulties to the identification of the same Chinese herbal medicines from different origins

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