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Biomarker for detecting osteoarthritis and application of biomarker

A biomarker, osteoarthritis technology, applied in the field of biomedicine, can solve problems such as deficiency

Active Publication Date: 2018-05-18
PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, due to the lack of fundamental understanding of the etiology of OA, the medical community still lacks effective drug means for its radical cure, and joint replacement surgery often becomes the final treatment that patients must choose

Method used

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  • Biomarker for detecting osteoarthritis and application of biomarker
  • Biomarker for detecting osteoarthritis and application of biomarker
  • Biomarker for detecting osteoarthritis and application of biomarker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The collection of embodiment 1 sample

[0047] Case-control study is the method used in this study. The research subjects of the experimental group were 4 patients with severe osteoarthritis who were hospitalized in the Department of Orthopedics of Peking Union Medical College Hospital in 2015 but had not yet received surgical treatment, and the research subjects of the control group were 4 relatively healthy people without osteoarthritis , the experimental group met the diagnostic criteria for osteoarthritis of the Orthopedic Branch of the Chinese Medical Association. The age of the experimental group was 58.5±2.0 years old (56-61 years old), and the age of the control group was 57.6±2.1 years old (55-61 years old). According to the "Adult Weight Judgment" standard formulated by the National Health and Family Planning Commission of the People's Republic of China in 2013, both groups selected overweight individuals with a BMI between 24 and 28. The experimental protoc...

Embodiment 2

[0058] Example 2 iTRAQ experiment screening differential proteins

[0059] 1. Instruments and reagents used

[0060] Vortex oscillator (Haimen Qilinbeier Instrument Manufacturing Co., Ltd., model: QL-901)

[0061] Centrifuge (Thermo, model: PICO17 )

[0062] Ultrasonic cell disruptor (Nanjing Xianou Instrument Manufacturing Co., Ltd., model: XO)

[0063] Microplate reader (Thermo, model: MultiskanMK3 )

[0064] Constant temperature incubator (Shanghai Pudong Rongfeng Scientific Instrument Co., Ltd., model: HH.S4)

[0065] Vacuum freeze dryer (Thermo, model: SPD2010-230 )

[0066] RIGOL L-3000 HPLC system (Beijing Puyuan Jingdian Technology Co., Ltd.), mobile phase A: 98% ddH2O, 2% acetonitrile (pH 10); mobile phase B: 98% acetonitrile, 2% ddH 2 O (pH 10) high performance liquid chromatography: (ThermoScientic EASY-nLC 1000System (Nano HPLC)), mobile phase A: 100% ultrapure water, 0.1% formic acid; mobile phase B: 100% acetonitrile, 0.1% formic acid mass spectrometry syste...

Embodiment 3

[0132] Example 3 ELISA verification of the expression of CRISP3 and CALML3 proteins in patients with osteoarthritis

[0133] 1. Sampling

[0134]Referring to the standard for selecting samples in Example 1, 10 cases of osteoarthritis patients were selected in the Department of Orthopedics of Peking Union Medical College Hospital in 2015, respectively labeled as A1-A10, and the subjects of the control group were relatively healthy people who did not suffer from osteoarthritis 10 The examples are labeled B1-B10 respectively. Collect 20ml of the second mid-morning urine from the experimental group and the control group, first put it in a sterile wide-mouth container, then transfer it to a centrifuge tube, centrifuge at 4,000g for 10min (4°C), take 1ml of the supernatant and put it in Store at -80°C for later use.

[0135] 2. Experimental process

[0136] Experimental selection of human CALML3 ELISA kit and human CRISP3 ELISA kit (purchased from Abcam)

[0137] The operation s...

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Abstract

The invention discloses a biomarker for detecting osteoarthritis. The biomarker comprises CRISP3 protein and / or CALML3 protein. The invention discloses the protein CRISP3 and / or CALML3 protein relatedto osteoarthritis, and down-regulated expression of the CRISP3 protein and / or CALML3 protein in urine is further confirmed. With the utilization of the protein for detecting osteoarthritis, rapid andeffective early detection can be achieved, and therapeutic targets and important basis are provided for clinical applications of gene therapy, drug therapy and the like.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a biomarker for detecting osteoarthritis and its application. Background technique [0002] Osteoarthritis (OA) is a chronic degenerative joint disease that is caused by multiple factors and is very common in middle-aged and elderly people. Joints can also be found in shoulder joints, wrist joints, ankle joints, etc. Osteoarthritis is characterized by wear and tear of articular cartilage, degeneration and bone hyperplasia around the joints, often accompanied by synovitis in the acute phase. According to the survey, the prevalence rate of osteoarthritis in the crowd over 60 years old is more than 50%, and the prevalence rate of the crowd over 75 years old is as high as 80%, and the disability rate of the disease can reach 53%. [0003] Osteoarthritis of the knee joint causes pain, swelling, stiffness, limited range of motion and dysfunction of the knee joint, which seriously affects t...

Claims

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Application Information

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IPC IPC(8): C07K14/47G01N33/558G01N33/68
CPCC07K14/47G01N33/558G01N33/68G01N2800/105
Inventor 朱乾坤邱贵兴吴志宏翁习生肖刻
Owner PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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