Extraction method of effective components of isatis roots

A technology of active ingredients and extraction method, applied in the field of extraction of active ingredients of Radix isatidis, can solve the problems of long heating time and destruction of active ingredients, etc.

Inactive Publication Date: 2018-05-18
ANHUI YONSENT PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for extracting effective components of Radix isatidis, which solves the problems of longer heating time for concentration after water extraction and alcohol precipitation after alcohol precipitation, and greater damage to active components. The extraction method is scientific and stable, and has strong practicability , suitable for industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Pre-extraction: Wash the Radix Radix and soak it for 1 hour, then decoct the soaked Radix Radix together with the soaking liquid for 2 hours, collect the decoction liquid, continue to add water to the dregs and decoct it for 1 hour, and combine the two decoction liquids. Radix isatidis pre-extraction, dregs dried and ground to obtain dregs powder;

[0025] (2) Microwave extraction: put 10kg Radix isatidis pre-extraction into the microwave extraction equipment, add the dregs powder and stir evenly, then add 800g 70% ethanol solution, 300g 10% ether solution and 50g 0.01% dihydrogen phosphate Sodium aqueous solution, turn on the microwave extraction equipment, and irradiate and extract for 5 minutes at a temperature of 35°C and a power of 300W to obtain the Radix Radix Radix Extract;

[0026] (3) Ultrafiltration separation and purification: Pass the Radix Radix extract into an organic ultrafilter, and under the conditions of a temperature of 5°C and a pressure of 0.2M...

Embodiment 2

[0030] (1) Pre-extraction: Wash the Radix Radix and soak it for 2 hours, then decoct the soaked Radix Radix and the soaking liquid for 3 hours, collect the decoction liquid, continue to add water to the dregs and decoct it for 1 hour, and combine the two decoction liquids to get the product Radix isatidis pre-extraction, dregs dried and ground to obtain dregs powder;

[0031] (2) Microwave extraction: put 10kg Radix isatidis pre-extraction into the microwave extraction equipment, add the dregs powder and stir evenly, then add 1.5kg 50% ethanol solution, 300g 10% ether solution and 120g 0.01% hydrogen phosphate Disodium aqueous solution, turn on the microwave extraction equipment, and irradiate and extract for 8 minutes at a temperature of 40°C and a power of 500W to obtain the extract of Radix Radix Radix;

[0032] (3) Separation and purification by ultrafiltration: Pass the Radix Radix extract into an organic ultrafilter, and at a temperature of 25°C and a pressure of 0.6Mpa,...

Embodiment 3

[0036] (1) Pre-extraction: Wash the Radix Radix and soak it for 1.5 hours, then decoct the soaked Radix Radix and the soaking liquid for 2.5 hours, collect the decoction liquid, add water to the dregs and decoct for 1 hour, and combine the two decoction liquids The isatis root pre-extraction solution is obtained, and the dregs are dried and ground to obtain the dregs powder;

[0037] (2) Microwave extraction: put 10kg Radix isatidis pre-extraction into the microwave extraction equipment, add medicinal dregs powder and stir evenly, then add 1kg 50% ethanol solution, 700g 10% ether solution and 80g 0.01% dihydrogen phosphate Sodium aqueous solution, turn on the microwave extraction equipment, and irradiate and extract for 6 minutes at a temperature of 36°C and a power of 400W to obtain the Radix Radix Radix extract;

[0038] (3) Separation and purification by ultrafiltration: Pass the Radix Radix extract into an organic ultrafilter, and at a temperature of 20°C and a pressure of...

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PUM

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Abstract

The invention discloses an extraction method of effective components of isatis roots. The method particularly includes the steps: pre-extraction; microwave extraction; ultra-filtration, separation andpurification; freeze drying. According to the method, a traditional extraction process of a water-extraction and alcohol-precipitation method is improved, isatis roots are decocted and pre-extractedand then further extracted, the microwave radiation process includes that high-frequency electromagnetic wave penetrate extracting media to reach microtubule bundles and glandular cell systems insidethe isatis roots, microwave energy is absorbed, so that the temperature in cells rapidly rises, the pressure in the cells exceeds bearable capacity of cell wall expansion, the cells are fractured, effective components in the cells freely flow out and are dissolved in the extracting media at low temperature, and the effective components in the isatis roots are sufficiently extracted in a short time.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine extraction, in particular to a method for extracting effective components of Radix isatidis. Background technique [0002] Banlangen Granules have the effects of clearing heat and detoxifying, cooling blood and relieving sore throat. It is used for sore throat and dry mouth and throat caused by excessive heat in the lung and stomach; acute tonsillitis sees the above syndromes. [0003] The current standard preparation method of Banlangen Granules in the National Pharmacopoeia is: decoct Banlangen twice, the first time for 2 hours, the second time for 1 hour, filter the decoction, combine the filtrates, concentrate to a relative density of 1.2 (50°C), and then Add ethanol to make the alcohol content reach 60%, let it stand for precipitation, take the supernatant, recover the ethanol and concentrate to an appropriate amount, add appropriate amount of auxiliary materials (dextri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/195A61K36/315A61K125/00
CPCA61K36/195A61K36/315A61K2236/331A61K2236/39A61K2236/51A61K2236/53
Inventor 程玉柱夏运喜刘晓燕
Owner ANHUI YONSENT PHARMA
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