Primer, plasmid and method for detection of mulberry mosaic type atrophy-associated viruses

Abstract

The invention discloses a primer, a plasmid and a method for the detection of mulberry mosaic type atrophy-associated viruses. The primer comprises an upstream primer MCP746f and a downstream primer MCP1148r, and nucleotide sequences of the upstream primer and the downstream primer are respectively shown as SEQ ID NO.1 and SEQ ID NO.2; meanwhile, the positive plasmid for detection is constructed,the fragment construction plasmid 13MCP-pEASY-Bblunt with a size of 1309 bp is obtained by the amplification of an upstream primer 13MCP156f and a downstream primer 13MCP1464r, and nucleotide sequences of the plasmid and the primers are respectively shown as SEQ ID NO.3, SEQ ID NO.4 and SEQ ID NO.5. The primer disclosed by the invention can specifically detect the mulberry mosaic type atrophy-associated viruses, is reliable in detection results, easy to operate, strong in specificity and high in sensitivity, particularly has an important practical application significance in the detection of diseased leaves at the early stage of infection (during the latent incubation period), meanwhile can detect pathogenic bacteria existing on leaves, petioles, buds and branches of diseased plants and has a practical guiding significance in the prevention and the control of the mulberry mosaic atrophy-associated viruses.

Description

technical field [0001] The invention relates to the technical field of pathogen molecule detection. More specifically, it relates to an application in the molecular detection of viruses associated with mulberry leaf wasting disease. Background technique [0002] Mulberry is an important economic crop in my country. With the comprehensive utilization of mulberry resources, in addition to being the main food for silkworms, mulberry has more food and medicinal value and ecological restoration functions. With the growth of the mulberry industry, mulberry diseases will gradually become the focus of our attention. [0003] As one of the most important mulberry virus diseases, mulberry mosaic leaf atrophy is common and serious in silkworm regions in my country. The disease has a great impact on the yield of mulberry leaves. The promotion of hybrid mulberry varieties has led to a decrease in the planting area of ​​disease-resistant varieties, which eventually led to a large increa...

AI Technical Summary

Problems solved by technology

Although the use of PCR technology to detect geminiviruses has been reported (Xie Yan et al., 2002, Ye Qingjing et al., 2009), but the virus associated with mulberry leaf wilt disease is a new species of geminiviruses (Ma Yuxin, 2015), and its PCR detection The technology needs further research, and the research of using PCR technology to detect mulberry virus has not been carried out so far.

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