Bacillus subtilis LPB-3, lipopeptide type biosurfactant and biodegradable environment-friendly glass cleaning liquid

A Bacillus subtilis, LPB-3 technology, applied in the direction of cationic surface active compounds, microorganisms, microorganisms, etc., can solve the problems of soil and groundwater pollution, soil and basement pollution, violation of environmental protection concepts, etc., to achieve environmental protection and low cost. , the effect of high yield

Active Publication Date: 2018-05-04
湖北绿瓜生物科技有限公司
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AI Technical Summary

Benefits of technology

The present invent describes methods for producing biocide products from Bacillus spp., which are used by farmers or researchers during their production processes. These techniques involve culturing cells that produce these substances while they grow on various substrates such as rocks or plants. This results in an increase in productivity compared to traditional ways of making them through different steps like extraction, concentration, sterilization, etc. Additionally, this method allows us to create pure forms of these compounds without any unwanted side reactions caused by previous means. Overall, this technology provides technical benefits over current methods but also offers environmental advantages due its ability to be made into nonbiodesoluble materials suitable for use in applications where easy decomposition cannot occur.

Problems solved by technology

The technical problem addressed in this patented text relating to improving the efficiency and effectiveness of biocide products like lactic acid ester compounds (lactoesters) due to its ability to reduce harmful effects on ecosystems while being able to actively promote growth.

Method used

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  • Bacillus subtilis LPB-3, lipopeptide type biosurfactant and biodegradable environment-friendly glass cleaning liquid
  • Bacillus subtilis LPB-3, lipopeptide type biosurfactant and biodegradable environment-friendly glass cleaning liquid
  • Bacillus subtilis LPB-3, lipopeptide type biosurfactant and biodegradable environment-friendly glass cleaning liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A ring of Bacillus subtilis LPB-3 strain was inoculated into LB test tube slant medium for activation, the culture temperature was 32°C, and the culture was statically cultured for 24 hours. The activated slant strain was transferred into a 2L shake flask for primary seed expansion cultivation at a cultivation temperature of 32° C., a shaking table rotation speed of 150 rpm, and a cultivation period of 10 h. According to the inoculum amount of 1%, the primary seed solution was put into a 10L seed tank for secondary seed expansion cultivation. The cultivation temperature was 32°C, the rotation speed was 100rpm, the ventilation rate was 1vvm, the tank pressure was 0.02MPa, and the cultivation period was 8h. According to the inoculum amount of 5%, the secondary seed solution was put into a 100L fermenter for fermentation and cultivation. The cultivation temperature was 32°C, the initial pH was 6.5, the rotation speed was 50rpm, the ventilation rate was 0.5vvm, the tank pres...

Embodiment 2

[0028]Take a ring of Bacillus subtilis LPB-3 strain and inoculate it in the slant medium of LB test tube for activation. The culture temperature is 35°C, and the culture is static for 24 hours. The activated slant strain was transferred into a 2L shake flask for primary seed expansion cultivation at a cultivation temperature of 35° C., a shaking table rotation speed of 200 rpm, and a cultivation period of 15 hours. Put the primary seed liquid into a 10L seed tank according to the inoculation amount of 4% for secondary seed expansion cultivation, the cultivation temperature is 35°C, the rotation speed is 150rpm, the ventilation volume is 1.5vvm, the tank pressure is 0.04MPa, and the cultivation period is 12h. According to the inoculum amount of 8%, the secondary seed solution was put into a 100L fermenter for fermentation and cultivation. The cultivation temperature was 35°C, the initial pH was 7.0, the rotation speed was 100rpm, the ventilation volume was 1vvm, the tank pressur...

Embodiment 3

[0030] Take a ring of Bacillus subtilis LPB-3 strain and inoculate it in the slant medium of LB test tube for activation. The culture temperature is 35°C, and the culture is static for 24 hours. The activated slant strain was transferred into a 2L shake flask for primary seed expansion cultivation at a cultivation temperature of 35° C., a shaking table rotation speed of 150 rpm, and a cultivation period of 12 hours. Put the primary seed liquid into a 10L seed tank according to the inoculum amount of 5% for secondary seed expansion cultivation, the cultivation temperature is 35°C, the rotation speed is 100rpm, the ventilation volume is 1vvm, the tank pressure is 0.04MPa, and the cultivation period is 8h. According to 10% inoculum amount, the secondary seed liquid was put into a 100L fermenter for fermentation culture. The culture temperature was 35°C, the initial pH was 6.5, the rotation speed was 100rpm, the ventilation volume was 1vvm, the tank pressure was 0.04 MPa, and the c...

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Abstract

The invention discloses bacillus subtilis LPB-3, a lipopeptide type biosurfactant and biodegradable environment-friendly glass cleaning liquid. The bacillus subtilis LPB-3 is named as bacillus subtilis in a classified way; the bacterial strain is preserved in CGMCC (China General Microbiological Culture Collection Center) on December 4, 2017; the preservation number is CGMCC No.14375. The preparedbacillus subtilis is subjected to culture fermentation and separation and purification to obtain the lipopeptide type biosurfactant for replacing a chemical surfactant in a common glass water formula; other difficult-to-decompose organic matters and inorganic matters in the glass water formula are replaced by a biodegradable biological formula; the cleaning performance of the glass water is realized; meanwhile, the complete biodegradation can be reached; zero harm is caused on environment. Meanwhile, the preparation process of the biosurfactant is simple and efficient; the yield is high; thecost is low; the green and environment-friendly effects are realized.

Description

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Claims

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Application Information

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Owner 湖北绿瓜生物科技有限公司
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