Immunoassay method based on iron (II) and phenanthroline system and glucose-encapsulated liposome
An o-phenanthroline and immunoassay technology, which is used in material analysis by observing the effect on chemical indicators, analysis by chemical reaction of materials, and material inspection products, etc., to achieve convenient use, low price, and improved sensitivity. Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0034] 1. Preparation of Glucose-Loaded Liposomes (GLL)
[0035] First, hydrogenated soybean lecithin, cholesterol and phosphoethanolamine (molar ratio: 50:10:1) were added to a mixed solution of chloroform and ethanol (5.0 mL, 3:1, v / v) to dissolve. After sonicating for 5 minutes at room temperature, the mixture was kept under the protection of nitrogen for 45 o C Rotary evaporate until a well mixed film forms. Next, an excess of dextrose solution (5 mM, 10 mL) was injected by syringe, at 45 o Sonicate for 5 minutes at C. In order to obtain a suspension with uniform particle size distribution, the suspension was filtered with a 0.4 μm polycarbonate membrane several times. To remove free glucose molecules, the dispersion was dialyzed against deionized water for 2 h in a dialysis cassette (MWCO, 12–14 kDa). The finally obtained glucose-loaded liposomes (GLL) were redispersed in phosphate buffered saline (1.0 mL, pH 7.4) and stored at a temperature of 4 o C space is left fo...
PUM
Property | Measurement | Unit |
---|---|---|
particle diameter | aaaaa | aaaaa |
diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com