Method for analyzing influence of discovered protein marker on liver cancer

Abstract

The invention discloses a method for analyzing influence of a discovered protein marker on liver cancer. The method comprises the following steps: through real-time fluorescence quantitative RT-PCR and a Western blot technology, detecting a novel liver cancer marker in liver tissues of a liver cancer patient, a benign liver tumor patient and a normal person respectively, determining the expressionamount thereof in gene and protein levels and location thereof, and analyzing a relationship between the novel liver cancer marker and the clinical pathology of the liver cancer patient; constructingan eukaryotic expression vector of a novel liver cancer protein marker encoding gene with a fluorescent label, and transfecting expression of a liver cancer cell line; measuring cell characteristicsbefore and after transfection by using laser confocal microscopy and the like. The invention finds an effective tumor marker, which is of a great significance in research of a canceration mechanism, early diagnosis of a disease and prognostic judgment.

Description

technical field [0001] The invention relates to the fields of gene transcriptomics and proteomics, in particular to a method for analyzing the impact of discovered protein markers on liver cancer. Background technique [0002] In the 20th century, primary liver cancer has risen to become the second cancer killer in my country, second only to lung cancer in urban areas and gastric cancer in rural areas [1] . At present, about 35,000 people in Guangxi die of malignant tumors every year, among which, about 12,000 people die of liver cancer. It can be seen that liver cancer is the first cancer killer in Guangxi. In Guangxi, the hazards of liver cancer are very uneven. The counties and cities with high mortality rate of liver cancer are mainly concentrated in the southwestern part of Guangxi. The three counties of Fusui, Long'an and Wuming around Nanning have the highest incidence. The incidence density of liver cancer in this local area reaches 50-60 / 100,000. In some administ...

AI Technical Summary

Problems solved by technology

The high sensitivity of mass spectrometry can help people discover low-abundance proteins and peptides in biological samples in the stage of marker discovery. The dynamic range greatly affects the quantitative detection of low-abundance proteins and peptides in biological fluids by mass spectrometry

Although researchers have tried to use a variety of methods, including chromatographic separation as much as possible, removal of high-abundance proteins, etc., the precision and accuracy of the results are still far from meeting the requirements of analysis, until mass spectrometry multiple reaction monitoring (multiple reaction monitoring) The emergence of monitoring, MRM) technology