Familial deteriorating atrioventricular conduction block gene diagnosis kit

Abstract

The invention discloses a familial deteriorating atrioventricular conduction block gene diagnosis kit, and is characterized in that the invention relates to the diagnosis kit for detecting familial deteriorating atrioventricular conduction block genes by a large-scale parallel sequencing platform NGS technique. The kit is mainly characterized in that: A, a capture probe is uniquely designed and prepared and aims at all exon fragments of genes SCN5A and TRPM4; B, a connector with a label sequence is uniquely designed; C, a probe sequence is subjected to PCR amplification by universal primers; and D, a uniquely designed target fragment after mixing is subjected to capture operation.

Description

Technical field [0001] The invention belongs to the field of molecular biology, relates to medical diagnosis and biotechnology, relates to an in vitro diagnostic kit for familial deteriorating atrioventricular block gene mutations, and is a family of NGS technology applied to a new generation sequencing platform Gene diagnosis kit for sexually worsening atrioventricular block. Background technique [0002] Heart block is a permanent or temporary impulse conduction disorder caused by anatomy or malfunction. It can be divided into 3 degrees according to its severity. Common causes of complete atrioventricular block are drug action, hypertonic vagus nerve, coronary heart disease, electrolyte imbalance, endocarditis, tumor, mucinous edema, calcified aortic stenosis, polymyositis, myocardial infiltrating disease And open heart surgery. In adults, drug poisoning, coronary heart disease and degenerative changes are the most common causes. Coronary atherosclerosis is the primary cause...

AI Technical Summary

Problems solved by technology

[0003] As a traditional sequencing technology, Sanger sequencing can only determine one base sequence in one reaction. Although it is accurate, it is inefficient and the cost of single nucleotide sequencing is high.

For the research of complex diseases with genetic heterogeneity, it is usually necessary to sequence the entire coding regions of several or even dozens of genes to find valuable mutation information, and Sanger sequencing cannot meet the requirements of sequencing

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