A preparation method of a two-ion starch-based stem cell expansion hydrogel and a method for expanding and collecting stem cells

Abstract

The invention discloses a preparation method of a two-ion starch-based stem cell expansion hydrogel and a stem cell expansion and collection method. The hydrogel is prepared from metacrylic acid estermodified two-ion starch and double-ended sulfydryl modified polyethylene glycol in sulfydryl-alkene click means, and CGRGDS polypeptides are introduced in the preparation process, so that the affinity of the hydrogel for cells is improved. The mechanical strength of the hydrogel can be controlled within the range of 27.21 to 69.92 kPa through the content of a polymer. More importantly, the adhesion of nonspecific proteins in the cell expansion process can be effectively reduced. The hydrogel has high biocompatibility, the multiplication of brown fat stem cells can be supported, and the stem cell characteristic is maintained in the multiplication process. Solid technical support can be provided for tissue engineering and cell treatment strategy. The hydrogel is chemically obtained throughclicking, the participation of an organic solvent is eliminated, the environment is protected, and the disadvantage that the support biocompatibility is reduced, which is caused in the synthetic process, is reduced.

Description

technical field [0001] The invention relates to a stem cell expansion hydrogel and a preparation method thereof based on a two-ion starch hydrogel stem cell expansion and collection method, belonging to the field of biomedical engineering. Background technique [0002] Stem cells are the original source of the human body and its various tissue cells, and have the characteristics of high self-renewal ability, high proliferation and multi-lineage differentiation potential, implantability and reconstruction ability. It has good application prospects in cell therapy and tissue engineering, and its large-scale expansion is the premise and basis of its clinical application. In clinical treatment and tissue engineering applications, the demand is relatively large, generally at 10 9 -10 11 Among them, the traditional two-dimensional culture technology cannot realize the large-scale expansion of stem cells due to the low expansion efficiency. In recent years, the developed 3D cultu...

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Problems solved by technology

In clinical treatment and tissue engineering applications, the demand is relatively large, generally at 10 9 -10 11 Among them, the traditional two-dimensional culture technology cannot realize the large-scale expansion of stem cells due to the low expansion efficiency. In recent years, the developed 3D culture technology based on microcarriers has achieved the large-scale expansion of cells

However, there are still some problems that need to be solved. For example, it is easy to be interfered by non-specific proteins during the culture process, which makes it difficult to maintain the "stemness" of the cells during the expansion process, especially for some cells with self-differentiation ability (such as: Brown adipose stem cells with myocardial self-differentiation ability), this phenomenon is more obvious

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