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Luffa enzyme mixed liquid and preparation method thereof

A technology of mixed solution and loofah, applied in the field of loofah enzyme mixed solution and its preparation, can solve the problems of easy deterioration, short storage period of loofah stock solution, low utilization rate of the whole loofah plant, etc., and achieves the advantages of promoting human metabolism and excellent in vitro inhibitory effect. Effect

Active Publication Date: 2018-04-20
南京同仁堂健康产业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Fruit and vegetable enzymes for the purpose of eating or health care emerge in an endless stream on the market, and edible enzyme liquids prepared from loofahs are also available as raw materials, but the loofah enzyme mixture prepared by the present invention is mainly used as cosmetic and skin care products in the daily chemical industry. The use of functional ingredients in the product is focused on solving the problems of short storage period and easy deterioration of loofah stock solution when used as a daily chemical raw material, and the low utilization rate of the whole loofah plant, and the underutilization of loofah stems and leaves as waste
[0006] At present, there is a lack of a loofah enzyme mixture capable of inhibiting enzyme activity and a preparation method thereof

Method used

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  • Luffa enzyme mixed liquid and preparation method thereof
  • Luffa enzyme mixed liquid and preparation method thereof
  • Luffa enzyme mixed liquid and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] A kind of loofah ferment mixed solution of the present invention is characterized in that comprising the following components in parts by mass:

[0034]

[0035] A kind of preparation method of luffa ferment mixed liquid of the present invention, comprises the steps:

[0036] (1) Choose 500 grams of mature loofah, wash it with running water, drain the surface water, cut it into small pieces of about 1cm*1cm, add 4000 grams of deionized water, and beat it into a homogenate in a beater;

[0037] (2) Use starch medium alone (2% soluble starch, 2% peptone, 1% yeast extract, 0.2% sodium chloride, pH 6.0, sterilized at 121°C for 30 minutes) for 24 hours, Trichoderma using potato medium (Potato 20%, sucrose 2%, agar 2%, pH6.0, 121 ℃ sterilization 30min can) cultivate 24h, then these 5 kinds of bacteria are mixed into fermented seed liquid in equal proportion; The inoculated fermentation raw material liquid is added, and the compound bacterial species is a combination of ...

Embodiment 2

[0044] The difference between embodiment 2 and embodiment 1 is: a kind of luffa ferment mixed solution of the present invention, comprises the following component composition in mass parts:

[0045]

[0046] A kind of preparation method of luffa ferment mixed liquid of the present invention, comprises the steps:

[0047] (1) Choose 1,000 grams of mature loofah, wash it with running water, drain the surface water, cut it into small pieces of about 1cm*1cm, add 6,000 grams of deionized water, and beat it into a homogenate in a beater;

[0048] (2) Use starch medium alone (2% soluble starch, 2% peptone, 1% yeast extract, 0.2% sodium chloride, pH 6.0, sterilized at 121°C for 30 minutes) for 24 hours, Trichoderma using potato medium (Potato 20%, sucrose 2%, agar 2%, pH 6.0, sterilized at 121°C for 30min) cultivated for 24h, and the composite strains were Trichoderma, Actinomyces nocardia, Silicate bacteria, polymyxa Combination of five bacteria Paenibacillus and Photosynthetic ...

Embodiment 3

[0052] The difference between embodiment 3 and embodiment 1 is: a kind of luffa ferment mixed liquor of the present invention, comprises the following component composition in mass parts:

[0053]

[0054]A kind of preparation method of luffa ferment mixed liquid of the present invention, comprises the steps:

[0055] (1) Choose 900 grams of mature loofah, wash with running water, drain the surface water, and cut into small pieces of about 1cm*1cm; choose 100 grams of loofah stems and leaves, wash with running water, drain the surface water, and cut into about 1cm A small section; add 8000 grams of deionized water, beat into a homogenous slurry in a beater;

[0056] (2) Use starch medium alone (2% soluble starch, 2% peptone, 1% yeast extract, 0.2% sodium chloride, pH 6.0, sterilized at 121°C for 30 minutes) for 24 hours, Trichoderma using potato medium (Potato 20%, sucrose 2%, agar 2%, pH 6.0, sterilized at 121°C for 30 minutes) cultivated for 24 hours, and then six kinds ...

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Abstract

The invention discloses a luffa enzyme mixed liquid and a preparation method thereof. The preparation method comprises the following steps: (1) weighing all components at proportion, cleaning raw materials with running water, adding deionized water, and then performing pulping to obtain luffa juice; (2) adding saccharose to obtain an enzyme raw material liquid, and inoculating a composite strain;and (3) under a dark condition, performing sealed fermentation for 7-15 d at 20-30 DEG C to prepare the luffa enzyme mixed liquid. The luffa enzyme mixed liquid can inhibit the activity of tyrosinasein vitro and the activity of alpha-glucosidase in vitro, and has the effects of promoting human metabolism, maintaining beauty and keeping young, reducing blood fat, reducing blood glucose and the like.

Description

technical field [0001] The invention relates to the production field of enzymes, in particular to a luffa enzyme mixed solution and a preparation method thereof. Background technique [0002] Loofah is the fresh and tender fruit of loofah, a plant of Cucurbitaceae. Loofah contains a variety of nutrients needed by the human body, among which the content of calcium, phosphorus, iron and protein is the highest among melon vegetables. Its roots, vines, leaves, fruits, seeds, and network can be used as medicine, which has high medicinal value. The loofah wound liquid collected from the loofah stem, also known as loofah water, is rich in B vitamins to prevent skin aging, vitamin C to brighten the skin and other ingredients, which can maintain skin moisture, eliminate plaque, and make the skin white and tender. A rare beauty product, so loofah water is also known as "beauty water". [0003] Enzyme, originally intended to be a general term for thousands of enzymes involved in vari...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61Q19/00A23L33/00A23L33/135
CPCA61K8/97A61K2800/782A61K2800/85A61Q19/00A23L33/00A23L33/135
Inventor 单承莺马世宏蔡明勇聂韡张卫明
Owner 南京同仁堂健康产业有限公司
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