Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of derivatives containing arylpyrazole coumarin skeletons in inhibiting pathogenic fungi in edible mushrooms

A technology of coumarin skeleton and arylpyrazole, which is applied in the field of biochemical pharmacy, can solve problems such as adverse effects, mutagenesis, and teratogenicity, and achieve the effect of reducing large-scale use, reducing losses, and achieving good results

Inactive Publication Date: 2018-04-20
NANJING INST FOR THE COMPREHENSIVE UTILIZATION OF WILD PLANTS CHINA COOP
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the most effective way to control the diseases of edible fungi after picking is cold storage combined with chemical bactericidal preservatives. Although they can kill pathogenic bacteria and achieve the purpose of antiseptic preservation, many chemically synthesized preservatives have certain adverse effects on human health. There are carcinogenic, teratogenic, mutagenic, etc., so more and more voices require the development of safe, non-toxic, natural edible fungi preservatives
[0003] At present, there is a lack of application of a class of plant-derived derivatives containing arylpyrazolecoumarin skeletons in inhibiting pathogenic fungi of edible fungi

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of derivatives containing arylpyrazole coumarin skeletons in inhibiting pathogenic fungi in edible mushrooms
  • Application of derivatives containing arylpyrazole coumarin skeletons in inhibiting pathogenic fungi in edible mushrooms
  • Application of derivatives containing arylpyrazole coumarin skeletons in inhibiting pathogenic fungi in edible mushrooms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The application of a class of derivatives containing aryl pyrazole coumarin skeleton in edible mushroom fresh-keeping technology.

[0025] The application of a class of derivatives containing aryl pyrazole coumarin skeleton in inhibiting edible fungus pathogenic fungi.

[0026] The pathogenic fungus is Penicillium chrysogenum, Trichoderma viride, Aspergillus versicolor, Trichoderma or Aspergillus niger.

[0027] The described class of derivatives containing arylpyrazolecoumarin skeleton has a structure as shown in formula (I):

[0028]

[0029] Wherein, R is selected from: -H, -F, -Cl, -CH 3 、-OCH 3 , -OH, -NO 2 、-CF 3 、-OCH 2 CH 3 .

[0030] A class of derivatives containing an arylpyrazolecoumarin skeleton has a structure as shown in formula (II):

[0031]

[0032] A class of derivatives containing an arylpyrazolecoumarin skeleton has a structure as shown in formula (III):

[0033]

[0034] A class of derivatives containing an arylpyrazolecoumarin sk...

Embodiment 2

[0037] The biological activity evaluation method of the above-mentioned antibacterial compounds containing arylpyrazole coumarin skeleton is mainly plate test analysis, and according to the preliminary screening results, the dose-effect relationship of the bacteriostatic agent with better effect on pathogenic bacteria is determined. The specific instructions are as follows:

[0038] Common pathogens of edible fungi: Penicillium chrysogenum, Trichoderma sp., Aspergillus niger, T.viride, A.versicolor.

[0039] Preparation of pathogenic fungal spore suspension

[0040] Preparation of medium: PDA medium: 200g potato, 20g glucose, 20g agar powder, KH 2 PO 4 1g, MgSO 4 Dissolve 0.5g in 1000mL of distilled water, sterilize at 121°C for 20min and set aside.

[0041] Activation of five kinds of pathogenic fungi: each pathogenic fungus was inoculated into an appropriate amount of the above-mentioned culture medium from the laboratory cryopreservation bank, and cultivated overnight ...

Embodiment 3

[0044] Antifungal activity studies

[0045] Preparation of stock solution for screening antibacterial agents: 20 derivative compounds containing arylpyrazolecoumarin skeleton. Weigh 1mg of the compound, first dissolve it with 0.5mL ethanol, then dilute to 10mL with distilled water, and store it in a -20°C refrigerator for later use.

[0046] PDA plate, the compound and sterile water were prepared as 0.1 mg / mL bacteriostat solution, and sterile water was used as a negative control group. Use a pipette gun to add the prepared antibacterial agent into the PDA plate, add 0.5mL to each plate, use a glass coating rod to spread it evenly, and then air-dry it. Add a sterilized piece of filter paper with a diameter of 5mm to the suspension containing the spores of the pathogenic fungus, so that it is fully stained with the spores and hyphae of the pathogenic fungus. Inoculate the filter paper sheet with spores on the sample-containing plate, incubate at 20°C for 5-7 days, measure the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention discloses an application of derivatives containing arylpyrazole coumarin skeletons in inhibiting pathogenic fungi in edible mushrooms. The pathogenic fungi are penicillium chrysogenum, trichoderma viride, aspergillus versicolor, trichoderma, or aspergillus niger. The derivatives are safe, non-toxic, and good in effects of inhibiting pathogenic fungi, natural plant extracts containing structures of biological active ingredients for inhibiting pathogenic microorganisms, preventing microorganisms, inflammation and cancers, etc. are used, and the derivatives can reach effectsof extending shelf life of fruits and vegetables without using chemical preservatives.

Description

technical field [0001] The invention relates to the technical field of biochemical pharmacy, in particular to the application of a class of derivatives containing aryl pyrazole coumarin skeletons in inhibiting pathogenic fungi of edible fungi. Background technique [0002] Edible fungus has become one of the important ingredients that people like because of its rich nutritional value, delicious taste and unique taste. With the growth of edible fungus production, the requirements for efficient and safe fresh-keeping technology are also increasing. At present, the most effective way to control the diseases of edible fungi after picking is cold storage combined with chemical bactericidal preservatives. Although they can kill pathogenic bacteria and achieve the purpose of antiseptic preservation, many chemically synthesized preservatives have certain adverse effects on human health. Carcinogenic, teratogenic, mutagenic and other situations occur, so more and more voices require...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A23B7/154
CPCA23B7/154
Inventor 邵晨雯石宝俊林群英朱海亮
Owner NANJING INST FOR THE COMPREHENSIVE UTILIZATION OF WILD PLANTS CHINA COOP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com