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A kind of preparation method of nano gold, nano gold and application

A nano-gold, reaction technology, applied in the field of bioengineering, to achieve the effect of moderate size and wide adaptability

Active Publication Date: 2021-02-19
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on the synthesis of gold nanoparticles from bacterial extracts.

Method used

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  • A kind of preparation method of nano gold, nano gold and application
  • A kind of preparation method of nano gold, nano gold and application
  • A kind of preparation method of nano gold, nano gold and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Bacterial culture and preparation of nano-gold synthesis agent.

[0035] (1) Bacterial culture: The Deinococcus radiodurans with the preservation number of ATCC13939 was plated for activation and culture, and then a single colony was picked and inoculated in 5 mL of TGY medium, and cultured overnight at 32°C with a shaker, and the shaker rotating speed was 220rpm ; Then transfer to a large bottle medium (1L culture bottle containing 500mL TGY medium) at a ratio of 1:100, and cultivate for 24h under the conditions of 32°C and 220rpm to cultivate the bacteria to a stationary phase (OD600nm=1.0).

[0036] (2) Collection and washing of bacterial cells: After the bacteria are cultured to a stable stage, they are collected by centrifugation at 8000 g for 10 min to obtain fresh bacterial cells. It was resuspended in 0.01M phosphate buffer with pH 7.0, shaken sufficiently, and then centrifuged at 8000 g for 10 min to collect the bacterial cells.

[0037] (3) Broken cells and p...

Embodiment 2

[0040] Bacterial culture and preparation of nano-gold synthesis agent.

[0041] (1) Bacterial culture: The Deinococcus radiodurans with the preservation number of ATCC13939 was activated and cultured on a plate, then a single colony was picked and inoculated in 5 mL of TGY medium, and cultured overnight at 30°C with a shaker, and the shaker rotation speed was 220rpm. ; Then transfer to a large bottle medium (1L culture bottle containing 500mL TGY medium) at a ratio of 1:100, and cultivate the bacteria for 24h under the conditions of 30°C and 220rpm to cultivate the bacteria to a stationary phase (OD600nm=1.0).

[0042] (2) Collection and washing of bacterial cells: After the bacteria are cultured to a stable stage, they are collected by centrifugation at 8000 g for 10 min to obtain fresh bacterial cells. It was resuspended in 0.01M phosphate buffer with pH 7.0, shaken sufficiently, and then centrifuged at 8000 g for 10 min to collect the bacterial cells.

[0043](3) Broken ce...

Embodiment 3

[0046] Preparation of gold nanoparticles.

[0047] (1) Preparation of synthesis reaction solution: 1mM Au 3+ Solution (AuCl, HAuCl 4 ·3H 2 O) In a 50mL centrifuge tube, add an appropriate amount of Deinococcus radiodurans extract dry powder (prepared in Example 1, without ammonium sulfate treatment) to make its concentration 3.00mg / mL, at room temperature (pH 7, 25 ℃) Mix and react under the conditions.

[0048] (2) Monitoring the reaction process: with the progress of the reaction, the color of the solution changes, gradually becomes purple and becomes stable, and the wavelength is scanned after 6 hours of reaction (with a peak at 545 nm).

[0049] (3) Purification of nano-gold solution: filter the above purple solution with a 0.22 μm syringe filter, centrifuge at 15,000 g for 30 min, collect the precipitate, wash with distilled water for several times, and dialyze the dialysis bag with a cut-off of 14KD for 24 hours to remove the remaining Au 3+ Wait, collect the solutio...

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Abstract

The invention discloses a preparation method of nano-gold, nano-gold and application. A method for preparing nano-gold, comprising the following steps: (1) taking the supernatant after breaking the Deinococcus radiodurans thallus to make a nano-gold synthesis agent; (2) adding the nano-gold synthesis agent containing Au 3+ (3) collecting the precipitate and washing to obtain the nano gold. The present invention uses the extract of Deinococcus radiodurans to prepare nano-gold, which can efficiently, greenly and conveniently synthesize nano-gold, and the synthesized nano-gold has good purity, moderate size (about 51.72nm), negative charge on the surface and basically no cytotoxicity , using the extract of Deinococcus radiodurans as a synthetic metal nanomaterial gold has advantages that ordinary plant and bacterial extracts do not have.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a preparation method of nano-gold, nano-gold and application thereof. Background technique [0002] Gold is a rare precious metal with stable chemical properties. Due to its unique physical and chemical properties, it has shown strange and novel roles in decoration, electronics, instruments, and biomedical engineering. [0003] Nano-gold refers to the tiny particles of gold with a diameter of 1-100 nm. It has high electron density, dielectric properties and catalytic effect. It can be combined with a variety of biological macromolecules without affecting its biological activity. It has a wide range of applications in the fields of sensor, biochip, optics and biomedicine. Gold nanoparticles with different particle sizes can be easily prepared from chloroauric acid by reduction method, and the colors are red to purple depending on the diameter. [0004] In general, nanomat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P3/00C12R1/01
CPCC12P3/00
Inventor 田兵李九龙
Owner ZHEJIANG UNIV
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