Metschnikowia fructicola for preventing and controlling postharvest diseases of fruits and vegetables, and preparation method and using method of metschnikowia fructicola

A technology for drupe mechie yeast and post-harvest disease, applied in the field of drupe mecca yeast, can solve the problems of biocontrol effect only being verified on a few fruits, lack of bacteriostatic spectrum strains, etc., and achieve significant social and ecological benefits and traits Stable and good application prospects

Inactive Publication Date: 2018-04-13
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, although there are nearly a hundred species of antagonistic yeasts reported at home and abroad, the biocontrol effects of most antagonistic yeasts have only been verified on a few fruits.
However, since the biocontrol effects of different strains of the same yeast are very different (Filonow et al., 1996), most of the antagonistic yeasts lack strains with broad antibacterial spectrum and stable effect.

Method used

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  • Metschnikowia fructicola for preventing and controlling postharvest diseases of fruits and vegetables, and preparation method and using method of metschnikowia fructicola
  • Metschnikowia fructicola for preventing and controlling postharvest diseases of fruits and vegetables, and preparation method and using method of metschnikowia fructicola
  • Metschnikowia fructicola for preventing and controlling postharvest diseases of fruits and vegetables, and preparation method and using method of metschnikowia fructicola

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Biological properties of Metschnikowia fructicola strain BY33

[0020] 1. Morphological features

[0021] (1) YPDA medium (1% yeast extract powder, 2% peptone, 2% glucose, 1.8% agar, sterilized at 121°C for 20 minutes) was cultured at 26°C for 48h, and the colonies were round and white with smooth and round edges. The cell shape is ellipsoidal.

[0022] (2) After culturing in YPDA liquid medium for 24 hours, no mold was formed, the bacterial solution was turbid, and there was precipitation. Microscopically, the yeast cells were oval and budded.

[0023] 2. Molecular biological identification

[0024] Use the universal forward primer NL-1 (5'-GCATATCAATAAGCGGAGGAAAAG-3') and reverse primer NL-4 (5'-GGTCCGTGTTTCAAGACGG-3') to perform PCR amplification of yeast 26S rDNA D1 / D2 region nucleic acid sequence, and the PCR product The sequencing results were entered into the website www.NCBI.nlm.nih.gov for BLAST, the homologous sequences were downloaded from the G...

Embodiment 2

[0026] Implementation Example 2 The Inhibitory Effect of Drupe Metchiella BY33 on Apple Penicillium, Botrytis Botrytis and Black Spot

[0027] 1. Experimental protocol

[0028] Take out Metschnissiae BY33 from the -80°C refrigerator, activate it with YPDA medium (10g of yeast extract powder, 20g of peptone, 20g of glucose, 18g of agar, 1000ml of deionized water, natural pH, sterilized at 121°C for 30min), and pick Single colonies were placed in YPD liquid medium, cultivated at 26°C and 200r / min for 24h, centrifuged at 4000rpm for 5min, discarded the supernatant, washed the collected bacteria with sterile water for 3 times, and counted on a hemocytometer to prepare The concentration is 1×10 8 Cells / mL of Metchione druposum BY33 bacterial suspension.

[0029]Activate Penicillium expansum, Botrytis cinerea or Alternaria tenuissima on PDA medium plate, culture at 26°C for 7-14 days, scrape appropriate amount of spores, wash with sterile water Prepared to a concentration of 5 x ...

Embodiment 3

[0039] Implementation example 3 Inhibitory effect of drupe metchi yeast BY33 on pear fruit blue mold and gray mold

[0040] 1. Experimental protocol

[0041] Take out Metschnissiae BY33 from the -80°C refrigerator, activate it with YPDA medium (10g of yeast extract powder, 20g of peptone, 20g of glucose, 18g of agar, 1000ml of deionized water, natural pH, sterilized at 121°C for 30min), and pick Single colonies were placed in YPD liquid medium, cultivated at 26°C and 200r / min for 24h, centrifuged at 4000rpm for 5min, discarded the supernatant, washed the collected bacteria with sterile water for 3 times, and counted on a hemocytometer to prepare The concentration is 1×10 8 Cells / mL of Metchione druposum BY33 bacterial suspension.

[0042] Activate Penicillium expansum or Botrytis cinerea on a PDA medium plate, culture at 26°C for 7-14 days, scrape appropriate amount of spores, and prepare a concentration of 5×10 with sterile water. 4 cells / mL of Penicillium or Botrytis cine...

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Abstract

The invention discloses a metschnikowia fructicola strain BY33, which is wide in antibacterial spectrum and stable in effects, for preventing and controlling postharvest diseases of fruits and vegetables, and a using method and use of the metschnikowia fructicola strain BY33. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC), and has a preservation number of CGMCC No. 14908. The using method of the metschnikowia fructicola comprises the following steps: activating a strain, carrying out fermentation culture on the strain with YPD, centrifuging, and preparing thallus into 1*10<8> cells / mL bacterial suspension with sterile water; putting fruits and vegetables such as apples, pears, grapes, strawberries, citrus or cherry tomatoes into the bacterial suspension, soaking for 30s and then taking out, and drying in the air; putting the dried products into preservation boxes, and storing at room temperature. The metschnikowia fructicola strain can be used for controlling apple penicillium, gray mold and black spot, pear fruit penicillium and gray mold, grape gray mold, aspergillosis, fusarium mildew and anthracnose, strawberry gray mold and aspergillosis, citrus penicillium as well as cherry tomato gray mold and aspergillosis at the same time, and reduces the loss caused by the postharvest diseases, thus having a very good application prospect.

Description

technical field [0001] The invention relates to the field of biological control of post-harvest diseases of fruits and vegetables, in particular to a Metschnikowia fructicola strain used for biological control of post-harvest diseases of fruits and vegetables. The main postharvest diseases have significant control effect. Background technique [0002] Although the quality deterioration of fresh fruits and vegetables is affected by many factors, disease is the main reason. Among them, rot and deterioration caused by fungal diseases is the most serious factor in postharvest fruit loss. Although postharvest diseases of fruits and vegetables can be prevented and controlled in many ways such as agricultural control, physical control, chemical control and biological control, the main measure at present is chemical control (Eckert & Ogawa, 1985, 1988). However, the long-term use of chemical pesticides not only leads to the development of drug resistance of pathogenic bacteria but...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16A23B7/155A01N63/04A01P3/00C12R1/645
CPCA23B7/155A23V2002/00A01N63/30C12N1/145C12R2001/645A23V2200/10
Inventor 王友升黄津津马琳任向峰姚婷李丽萍
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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