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Hericium erinaceus protein-containing microbial preparation, as well as preparation method and application thereof

A microbial preparation, the technology of Hericium erinaceus, applied in the field of microecological preparations and its preparation, can solve the problems of less research on Hericium erinaceus protein, achieve a significant synergistic auxiliary effect, and improve the effect of gastrointestinal function

Inactive Publication Date: 2018-04-13
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the research on Hericium erinaceus at home and abroad mainly focuses on the extraction of Hericium erinaceus polysaccharides and the determination of chemical components, but there are few studies on Hericium erinaceus protein

Method used

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  • Hericium erinaceus protein-containing microbial preparation, as well as preparation method and application thereof
  • Hericium erinaceus protein-containing microbial preparation, as well as preparation method and application thereof
  • Hericium erinaceus protein-containing microbial preparation, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Preparation of Hericium Hericium Protein Extract

[0033] Specific steps are as follows:

[0034] (1) Crush the fruiting bodies of Hericium erinaceus with an ice crusher, add 20% distilled water, mix well and put them in a -80°C refrigerator for 6 hours. After the fruiting bodies of Hericium erinaceus are completely frozen, place them in a water bath at 37°C Freeze and thaw completely, repeat freeze-thaw 3 times;

[0035] (2) Put the frozen-thawed Hericium erinaceus fruiting bodies into a large beaker, add NaOH solution, adjust the pH to 9.47, keep the material-liquid ratio at 50ml / g, seal with plastic wrap, and extract with magnetic stirring at 37.9°C for 6h , repeatedly extracted twice, filtered, and combined filtrate;

[0036] (3) Adjust the pH to 4.5 with dilute hydrochloric acid solution to make the precipitation complete. Under the condition of 4°C, the number of revolutions is 8000r / min, the centrifugation time is 20min, and the precipitation is collected, whic...

Embodiment 2

[0038] Example 2: Preparation of probiotics containing Hericium erinaceus protein and compound probiotics

[0039] Get 30 parts by weight of Hericium erinaceus protein extract, 3 parts by weight of each probiotic dry powder of Bifidobacterium, Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus jansii, Streptococcus faecalis, Lactococcus, Streptococcus intermediarius, respectively 1.5ⅹ10 9 The number of viable bacteria / g, mix evenly, and then pack.

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Abstract

The invention discloses a microbial preparation and a preparation method thereof, in particular to a hericium erinaceus protein-containing microbial preparation and a preparation method thereof. The hericium erinaceus protein-containing microbial preparation is obtained by uniformly mixing hericium erinaceus protein extract and compound probiotics in the weight ratio ranging from 8:1 to 15:1; thecompound probiotics comprise a mixture of bifidobacteria, lactobacillus acidophilus, lactobacillus casei, lactobacillus jensenii, streptococcus faecalis, galactococcus and intermediate streptococcocciwhich are in the weight ratio of (1 to 2):(1 to 2):(1 to 2):(1 to 2):(1 to 2):(1 to 2):(1 to 2); the activities of the bifidobacteria, the lactobacillus acidophilus, the lactobacillus casei, the lactobacillus jensenii, the streptococcus faecalis, the galactococcus and the intermediate streptococcocci are 5*10<9> to 1*10<10> viable bacteria / g; the preparation method of the hericium erinaceus protein-containing microbial preparation comprises the steps which are shown in the specification; the microbial preparation disclosed by the invention can remarkably improve a gastrointestinal function; compared with using the compound probiotics, for example, the compound probiotics provided by the invention, singly, the microbial preparation has a remarkable synergistic assisting effect.

Description

technical field [0001] The invention relates to a microecological preparation and a preparation method thereof, in particular to a microbial preparation containing Hericium erinaceus protein, a preparation method and application thereof. Background technique [0002] Hericium erinaceus (Hericium erinaceus) is an edible fungus with great nutritional, health and medicinal value, flat in nature and sweet in taste. Studies have reported that the active substances of Hericium erinaceus are mainly polysaccharides, sterols (ergosterols), terpenes (diterpenes, heteroterpenes), fatty acids (saturated fatty acids, unsaturated fatty acids, etc.), phenols, proteins and other compounds. Benefits the internal organs, helps digestion, nourishes, anti-cancer, anti-neurasthenia and other effects. [0003] At present, the research on Hericium erinaceus at home and abroad mainly focuses on the extraction of Hericium erinaceus polysaccharides, the determination of chemical components, etc., bu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/02C07K1/14A61P1/00A61P29/00A61P37/04A61P35/00A61K35/745A61K35/747A61K35/744A61K36/07
CPCA61K35/744A61K35/745A61K35/747A61K36/07A61K38/02C07K1/145A61K2300/00
Inventor 陈地灵杨俭帅欧唐晓萃郑超群谢意珍吴清平杨柏华
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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