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A tissue culture method of endangered Tibetan medicinal material Mirabilis Himalayan

A technology for tissue culture and Tibetan medicinal materials, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of low yield, long reproduction cycle, and seasonal restrictions on seedling reproduction, and achieve fast reproduction and reproduction cycle. The effect of short, shortened growth cycle

Active Publication Date: 2020-05-22
VEGETABLE RES INST OF TIBET ACADEMY OF AGRI & ANIMAL HUSBANDRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The propagation cycle of the above-mentioned planting method is long, the yield is low, and seedling breeding is limited by seasons

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] This embodiment provides a method for tissue culture of the endangered Tibetan medicinal material Mirabilis Himalayan, the steps of which include:

[0029] (1) Select high-quality plump seeds, first use ethanol with a volume concentration of 60% to quickly rinse, and then use a concentration of 0.05% HgCl 2 The solution was disinfected for 3 minutes;

[0030] (2) The seeds treated in step (1) are quickly rinsed with sterile water for 3 times, and then the water on the surface of the seeds is blotted dry to obtain sterile explants for use;

[0031] (3) Under sterile conditions, inoculate the sterile explants described in step (2) onto 1 / 2 Ms medium, culture in dark at 15°C for 7 days until the seeds germinate, and transfer to Ms+6-BA2mg / l+NAA0.05mg / l medium, cultivate and induce cluster buds at 18°C ​​under natural light to form cluster buds; the natural light time per day is 10h;

[0032] (4) Transfer the cluster buds to 1 / 2Ms+0.5NAAmg / l, cultivate and take root at 1...

Embodiment 2

[0035] This embodiment provides a method for tissue culture of the endangered Tibetan medicinal material Mirabilis Himalayan, the steps of which include:

[0036] (1) Select high-quality plump seeds, first use ethanol with a volume concentration of 80% to quickly rinse, and then use a concentration of 0.2% HgCl 2 The solution was disinfected for 5 minutes;

[0037] (2) The seeds treated in step (1) are quickly rinsed with sterile water for 5 times, and then the water on the surface of the seeds is blotted dry to obtain sterile explants for use;

[0038] (3) Under sterile conditions, inoculate the sterile explants described in step (2) onto 1 / 2 Ms medium, culture in dark at 20°C for 5 days until the seeds germinate, and transfer to Ms+6-BA2mg / l+NAA0.05mg / l medium, cultivate and induce cluster buds at 25°C under natural light to form cluster buds; the natural light time per day is 12h;

[0039] (4) Transfer the cluster buds to 1 / 2Ms+0.7NAAmg / l, cultivate and take root at 25°C...

Embodiment 3

[0042] This embodiment provides a method for tissue culture of the endangered Tibetan medicinal material Mirabilis Himalayan, the steps of which include:

[0043] (1) Select high-quality plump seeds, first use ethanol with a volume concentration of 70% to quickly rinse, and then use a concentration of 0.1% HgCl 2 The solution was disinfected for 4 minutes;

[0044] (2) The seeds treated in step (1) are quickly rinsed with sterile water for 4 times, and then the water on the surface of the seeds is blotted dry to obtain sterile explants for use;

[0045] (3) Under sterile conditions, inoculate the sterile explants described in step (2) on 1 / 2 Ms medium, culture in dark at 18°C ​​for 6 days until the seeds germinate, and transfer to Ms+6-BA2mg / l+NAA0.05mg / l medium, cultivate and induce cluster buds at 22°C under natural light to form cluster buds; the natural light time per day is 11h;

[0046] (4) Transfer the cluster buds into 1 / 2Ms+0.6NAAmg / l, cultivate and take root at 22...

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Abstract

The invention relates to a tissue culture method of the endangered Tibetan medicinal material Mirabilis Himalayas. The selected seeds are successively treated with ethanol and HgCl 2 The solution is sterilized, and then quickly rinsed with sterile water to obtain sterile explants, which are sequentially inoculated under aseptic conditions, cultivated to induce cluster buds, rooted, cultivated, transplanted and colonized, until the medicinal material of Mirabilis Himalayan is harvested. The tissue culture method of the present invention can produce seedlings every year, shorten the growth period, increase the yield, and can be harvested in advance; the tissue culture method of the present invention is obtained by simulating the wild ecological environment of Mirabilis Himalayan in the plateau area, and the cost is low, and the reproduction Fast, able to adapt to the unique environment of large temperature difference, low temperature and strong ultraviolet rays in the plateau area, and has fast seedling raising speed, good heredity, short breeding cycle, and the quality of the obtained Mirabilis Himalayan is far superior to the existing technology.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a tissue culture method of endangered Tibetan medicinal material Mirabilis Himalayan. Background technique [0002] Himalayan mirabilis is a plant of Miraraceae, one of the most commonly used Tibetan medicinal materials, produced in various Tibetan areas. "Jingzhu Materia Medica", "Tibetan Medicine Jingjing Materia Medica" and other Tibetan medicine classics record that there are three kinds of Himalayan mirabilis: white Himalayan mirabilis, red Himalayan mirabilis, and black Himalayan mirabilis. The medicine is white Himalayan mirabilis. [0003] Himalayan Mirabilis grows in uphill grass, wasteland or shrubland at an altitude of 2900-3400m. It is produced in eastern Tibet and the Himalayas, and is also distributed in Sichuan and Yunnan. In our region, it is mainly distributed in the Himalayas, Qamdo, Nyingchi, Lhasa, Shannan and other areas. After investig...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/008
Inventor 白玛玉珍李淑萍谭淑琼欧珠卫华许娟妮曾钰婷祁驰恒
Owner VEGETABLE RES INST OF TIBET ACADEMY OF AGRI & ANIMAL HUSBANDRY SCI
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