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Cultivation method for agaricus bisporus

A cultivation method and technology of Agaricus bisporus are applied in the field of cultivation of Agaricus bisporus, which can solve the problems of easy water loss, slow seed production rate, poor water retention, etc., so as to shorten the growth time, improve the vitality and enhance the water retention capacity. Effect

Inactive Publication Date: 2018-04-13
常州莱尚纺织品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a method for cultivating Agaricus bisporus in view of the slow seed production rate, poor water retention and easy loss of water in the current cultivation process of Agaricus bisporus

Method used

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  • Cultivation method for agaricus bisporus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Strain: Agaricus bisporus, preserved in the Culture Center of Huazhong Agricultural University.

[0037] Plate medium: by mass parts, take 150 parts of potatoes, 15 parts of sucrose, KH 2 PO 4 1 part, MgSO4·7H 2 O0.05 parts, 15 parts of agar, 1000 parts of water, the pH is natural.

[0038] First-level liquid culture medium: in parts by mass, take 30 parts of wort juice, 8 parts of corn flour, KH 2 PO 4 0.8 parts, MgSO 4 0.3 copies, VB 1 0.01 parts, 1000 parts of water, pH6.5±0.2.

[0039] Secondary strain fermentation medium: in parts by mass, take 18 parts of bran, 5 parts of corn flour, 1 part of egg white, KH 2 PO 4 0.6 parts, MgSO 4 0.3 copies, VB 1 0.01 parts, 0.05 parts of fresh edible oil, 1000 parts of water, pH6.5±0.2.

[0040] Solid stock culture medium: in parts by mass, take 900 parts of wheat, 10 parts of dung grass, 5 parts of lime, 5 parts of gypsum, 1500 parts of water, pH6.5±0.2.

[0041] Solid culture medium: by mass, take 800 parts of whe...

Embodiment 2

[0049] Strain: Agaricus bisporus, preserved in the Culture Center of Huazhong Agricultural University.

[0050] Plate culture medium: by mass parts, take 170 parts of potatoes, 17 parts of sucrose, KH 2 PO 4 1 part, MgSO4·7H 2 O0.07 parts, 17 parts of agar, 1000 parts of water, pH natural.

[0051] First-level liquid culture medium: in parts by mass, take 30m33 parts of wort, 9 parts of corn flour, KH 2 PO 4 1.0 parts, MgSO 4 0.4 copies, VB 1 0.01 parts, 1000 parts of water, pH6.5±0.2.

[0052] Secondary strain fermentation medium: by mass parts, take 19 parts of bran, 7 parts of corn flour, 1 part of egg white, KH 2 PO 4 0.7 parts, MgSO 4 0.4 copies, VB 1 0.01 parts, 0.06 parts of fresh edible oil, 1000 parts of water, pH6.5±0.2.

[0053] Solid stock culture medium: in parts by mass, take 950 parts of wheat, 13 parts of dung grass, 6 parts of lime, 7 parts of gypsum, 1500 parts of water, pH6.5±0.2.

[0054] Solid culture medium: by mass, take 850 parts of wheat, 8...

Embodiment 3

[0062] Strain: Agaricus bisporus, preserved in the Culture Center of Huazhong Agricultural University.

[0063] Plate medium: by mass parts, take 200 parts of potatoes, 20 parts of sucrose, KH 2 PO4 2 parts, MgSO4·7H 2 O0.1 part, 20 parts of agar, 1000 parts of water, pH natural.

[0064] First-level liquid culture medium: in parts by mass, take 35 parts of wort juice, 10 parts of corn flour, KH 2 PO 4 1.2 parts, MgSO 4 0.5 copies, VB 1 0.02 parts, 1000 parts of water, pH6.5±0.2.

[0065] Secondary strain fermentation medium: in parts by mass, take 20 parts of bran, 10 parts of corn flour, 3 parts of egg white, KH 2 PO 4 0.8 parts, MgSO 4 0.5 copies, VB 1 0.02 parts, 0.08 parts of fresh edible oil, 1000 parts of water, pH6.5±0.2.

[0066] Solid stock culture medium: in parts by mass, take 1000 parts of wheat, 15 parts of dung grass, 8 parts of lime, 10 parts of gypsum, 1500 parts of water, pH6.5±0.2.

[0067] Solid culture medium: by mass, take 900 parts of wheat, 9...

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Abstract

The invention relates to the field of cultivation of mushrooms, and in particular relates to a cultivation method for agaricus bisporus. According to the method provided by the invention, expanded vermiculite is obtained through modification of vermiculite, the expanded vermiculite has very strong water absorbing performance, through the ion exchange ability of the vermiculite, cover soil can be loosened, the formation of a primordium of a sporocarp of the agaricus bisporus is facilitated, the problem that hyphae have reduced activity due to hypoxia caused by poor ventilation is improved, andthe water retaining capacity is enhanced; polyglutamic acid is added, and a mixture of the polyglutamic acid and the modified vermiculite is spread between the hyphae and the cover soil, in the process that the hyphae grow to a cover soil layer, through water dissolution, a film is formed at the surface layer of the hyphae, so that the nutrients and moisture in the cover soil can be in contact with and conveyed to the hyphae, the dissolution, storage, conveying and absorption of the nutrients can be extremely efficiently improved, and the producing rate of the agaricus bisporus can be improved.

Description

technical field [0001] The invention relates to the field of mushroom cultivation, in particular to a method for cultivating Agaricus bisporus. Background technique [0002] Soil is the base camp of microorganisms. The soil contains specific factors that are lacking in the growth and metabolism of microorganisms under laboratory conditions. Therefore, through the method of in situ cultivation, "unculturable microorganisms" can also grow under laboratory conditions. Adding soil water-soluble extracts to the medium can increase the expression of known genes of bacteria and actinomycetes and activate silenced genes. However, the effects of soil extracts on fungi have not been reported. [0003] Agaricus bisporus is the most widely cultivated edible fungus with the largest production and consumption in the world, and its output accounts for about 3 / 4 of the world's total output of edible fungi. Commonly used parent medium of Agaricus bisporus is potato dextrose agar medium...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/20
Inventor 吴保平李璐尹凯欣
Owner 常州莱尚纺织品有限公司
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