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Applications of benzopyran lactone in attenuating toxicity of aristolochic acid and traditional Chinese medicines containing aristolochic acid

A technology of benzopyran lactone and aristolochic acid, applied in the field of medicine, can solve the problems such as not being found

Active Publication Date: 2018-04-10
威海华洋药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, many Chinese medicines containing aristolochic acid have special uses in the field of traditional Chinese medicine, and there is no substitute for these traditional Chinese medicines containing aristolochic acid.
This has led to the fact that some classical prescriptions that have been passed down for hundreds of thousands of years can no longer be used because of the nephrotoxicity of aristolochic acid. It is a pity.

Method used

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  • Applications of benzopyran lactone in attenuating toxicity of aristolochic acid and traditional Chinese medicines containing aristolochic acid
  • Applications of benzopyran lactone in attenuating toxicity of aristolochic acid and traditional Chinese medicines containing aristolochic acid
  • Applications of benzopyran lactone in attenuating toxicity of aristolochic acid and traditional Chinese medicines containing aristolochic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Protective effect on human renal tubular epithelial cell damage caused by aristolochic acid

[0027] 1. Experimental materials

[0028] Human renal tubular epithelial cell line (HK-2) was purchased from China Type Culture Collection;

[0029] Aristolochic acid-I (AA-I) was purchased from China Institute for the Control of Pharmaceutical and Biological Products;

[0030] Fetal calf serum (FCS), Gibico company; DMEM: HamsF-12 medium (Hyclone); Annexin-V kit, Invitrogen company;

[0031] Enzyme-linked immunoassay instrument, American Thermofisher Company; flow cytometer, American BD Company.

[0032] 2. Experimental method

[0033] 1. Cell culture and grouping

[0034] Well-grown HK-2 cells were taken, suspended in DMEM: HamsF-12 medium containing 10% FCS and 1% penicillin + streptomycin, and 6×10 7 / L cell suspension was inoculated in a 96-well plate, inoculated 100 μL per well, at 37°C, 5% CO 2 After the cells adhered to the wall for 24 hours in an envir...

Embodiment 2

[0057] Example 2: Protective effect on aristolochic acid-induced renal interstitial fibrosis in rats

[0058] 1. Experimental materials

[0059] SPF grade male SD rats, weighing 280-300g, were purchased from Qinglong Mountain Animal Breeding Farm, Jiangning District, Nanjing;

[0060] Aristolochic acid Ⅰ was dissolved in edible oil as a working solution for intragastric administration.

[0061] 2. Experimental method

[0062] 1. Establishment of aristolochic acid renal fibrosis rat model and experimental grouping

[0063] Aristolochic Acid Renal Fibrosis Modeling Method (Reference: Establishment of Chronic Aristolochic Acid Nephropathy Animal Model and Taurine's Early Protective Effect on It, Chinese Journal of Integrated Traditional Chinese and Western Medicine, Vol. , Department of Pathology, Peking University Health Science Center): First, the rats were given a dose of 20 mg / kg / d aristolochic acid Ⅰ for 5 days continuously, and the drug was stopped for 9 days; after that...

Embodiment 3

[0085] Embodiment 3: make pharmaceutical composition with single flavor Chinese medicine

[0086] A pharmaceutical composition, which is a combination of a single Chinese medicine and one or more of peturogastrodin A, peturogastrodin B, peturogastrodin C, and peturogastrodin; the single herbal medicine can be Guan Mutong, Aristolochia , Fangji, Qingmuxiang, cinnabar lotus, Xugufeng, Tianxianteng, Magnolia officinalis, Asarum and other traditional Chinese medicines containing aristolochic acid.

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Abstract

The invention discloses applications of benzopyran lactone in attenuating the toxicity of aristolochic acid and traditional Chinese medicines containing aristolochic acid. The benzopyran lactone provided by the invention can attenuate the renal toxicity of aristolochia acid, and can be used for preparing attenuation medicines for single traditional Chinese medicines, traditional Chinese medicinalextracts or traditional Chinese medicinal compound preparations containing aristolochic acid. Benzopyran lactone can be developed into single medicines to be taken together with the single traditionalChinese medicines, traditional Chinese medicinal extracts or traditional Chinese medicinal compound preparations containing aristolochic acid, or the single traditional Chinese medicines, the traditional Chinese medicinal extracts or the traditional Chinese medicinal compound preparations containing aristolochic acid can be developed into medicinal compositions with benzopyran lactone. The traditional Chinese medicines can be the traditional Chinese medicines, such as caulis aristolochiae manshuriensis, fructus aristolochiae, radix aristolochiae fangchi, radix aristolochiae, radix aristolochiae kaempferi, herba aristolochiae mollissimae, herba aristolochiae, cortex magnoliae officinalis and herba asari, which contain aristolochic acid. The traditional Chinese medicinal compound preparations can be the traditional Chinese medicinal compound preparations such as rheum officinale pills for clearing stomach heat, children-welfare tablets, lucidity-separating pills for treating five kindsof stranguria, gentian pills for purging liver-fire, resurrection pills containing 10 ingredients named with Chinese character 'Xiang', and styrax pills for treating coronary heart diseases, which contain aristolochic acid.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to the use of benzopyranolactone for attenuating aristolochic acid and traditional Chinese medicines containing aristolochic acid. Background technique [0002] On October 18, 2017, researchers from Singapore and Taiwan published a research paper entitled "Aristolochic acids and their derivatives are widely implied in liver cancers in Taiwan and throughout Asia" in Science Translational Medicine, a sub-journal of Science. The article pointed out the dark side (toxic and side effects) of Chinese herbal medicine in the form of a cover story, and its main character—aristolochic acids (AA), is a class of chemical compounds widely present in Aristolochiaceae plants and various natural herbal medicines. Element. The chemical structures of the four main aristolochic acids are shown below: [0003] [0004] my country's State Food and Drug Administration (CFDA) and the medical community are also ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/37A61P13/12
CPCA61K31/37
Inventor 赵腾骅林溪王倩付俊毅
Owner 威海华洋药业有限公司
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