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Method for preparing nanofiber membrane with core/shell wrapping structure through coaxial dual-layer electrostatic spinning and application thereof

A nanofiber membrane and electrospinning technology, applied in the field of biomedical composite materials, can solve the problems of bionic wound dressing function, bionic skin extracellular matrix, etc., achieve good biocompatibility and biodegradability, raw materials Rich in resources, beneficial to the effect of gas exchange

Inactive Publication Date: 2018-04-06
佛山今兰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The natural skin extracellular matrix is ​​composed of polysaccharides and proteins with a three-dimensional nanofiber network structure. The current common wound dressings are based on electrospinning technology to mimic the three-dimensional network structure of the skin extracellular matrix, using natural or synthetic polymers as raw materials to simulate The composition of the extracellular matrix; however, the wound repair process is a complex dynamic process, adding a single material cannot completely biomime the extracellular matrix of the skin, so it is impossible to realize the functional biomimetic of the wound dressing

Method used

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  • Method for preparing nanofiber membrane with core/shell wrapping structure through coaxial dual-layer electrostatic spinning and application thereof
  • Method for preparing nanofiber membrane with core/shell wrapping structure through coaxial dual-layer electrostatic spinning and application thereof
  • Method for preparing nanofiber membrane with core/shell wrapping structure through coaxial dual-layer electrostatic spinning and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Take by weighing 0.2g PCL and 0.2g type I collagen (the mass ratio of PCL and type I collagen is 1:1) with electronic balance, be dissolved in 5ml hexafluoroisopropanol, obtain mass volume ratio concentration and be 8% (m / v ) core layer solution; Weigh 1.425g PLGA and 0.075g fibrinogen (PLGA and fibrinogen mass ratio is 19:1), dissolve in 5ml mixed solution (chloroform: PBS=9:1), get Shell solution with mass volume ratio concentration of 30% (m / v). The solution was magnetically stirred at room temperature for 12 hours to dissolve completely, and then coaxial electrospinning was started after standing for 5 minutes; the parameters of electrospinning were: electrostatic voltage 30kV, receiving distance 12cm, core layer solution propulsion rate 0.05ml / h, shell layer solution The propulsion rate is 0.1ml / h, and the relative humidity is 50%.

Embodiment 2

[0031] Take by weighing 0.16g PVP and 0.04g type I collagen (the mass ratio of PVP and type I collagen is 4:1) with electronic balance, be dissolved in 5ml hexafluoroisopropanol, obtain mass volume ratio concentration and be 4% (m / v ) core solution; Weigh 0.375g PLLA and 0.375g fibrinogen (the mass ratio of PLLA and fibrinogen is 1:1), dissolve in 5ml mixed solution (DMF:DCM:MEM=2:7:1) , to obtain a shell solution with a mass volume ratio concentration of 15% (m / v). The solution was magnetically stirred at room temperature for 12 hours to dissolve completely, and after standing for 5 minutes, coaxial electrospinning was started; the parameters of electrospinning were: electrostatic voltage 23kV, receiving distance 18cm, core solution advancing rate 1ml / h, shell solution advancing Rate 2.7ml / h, relative humidity 70%.

Embodiment 3

[0033] Take by weighing 0.54g PGA and 0.06g type I collagen (the mass ratio of PGA and type I collagen is 9:1) with electronic balance, be dissolved in 5ml hexafluoroisopropanol, obtain mass volume ratio concentration and be 12% (m / v ) core layer solution; Weigh 1.035g PLA and 0.115g fibrinogen (PLA and fibrinogen mass ratio is 9:1), dissolve in 5ml mixed solution (THF: normal saline=9:1), get the mass A shell solution with a concentration of 23% (m / v) by volume. The solution was magnetically stirred at room temperature for 12 hours to dissolve completely, and after standing for 5 minutes, coaxial electrospinning was started; the parameters of electrospinning were: electrostatic voltage 16kV, receiving distance 8cm, core solution advancing rate 0.2ml / h, shell solution The propulsion rate is 0.5ml / h, and the relative humidity is 20%.

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Abstract

The invention discloses a method for preparing a nanofiber membrane with a core / shell wrapping structure through coaxial dual-layer electrostatic spinning and application thereof, and belongs to the field of biomedical composite materials. The method adopts the coaxial dual-layer electrostatic spinning technology for preparing the nanofiber membrane with the core / shell wrapping structure, main components in a skin extracellular matrix are adopted as raw materials, the core is a degradable synthetic macromolecular material carrying type-I collagen, and the shell is a degradable synthesis macromolecular material carrying fibrinogen. The preparation method is simple and easy to implement, and the prepared nanofiber membrane has the good biocompatibility and the biodegradability; along with degradation of the shell layer material in the nanofiber and exposure of the core layer material, component change dynamic bionics in the skin wound repairing process can be achieved, normal skin tissuegeneration is promoted, and the good application prospect is achieved in the aspect of skin wound dressing.

Description

technical field [0001] The invention belongs to the field of biomedical composite materials, and in particular relates to a method for preparing a nanofiber membrane with a core / shell embedding structure by coaxial double-layer electrospinning, and its application as a bionic skin wound repair dressing. Background technique [0002] Skin tissue is located on the surface of the human body, and it is vulnerable to damage from the outside world in daily life, such as burns, wounds, ulcers, inflammation, and external stimulation, which cause tissue defects or ruptures, thereby forming wounds. At the same time, with the aging of the social population, the incidence of chronic diseases such as diabetes has gradually increased in many countries, and the refractory ulcer wounds caused by chronic diseases such as diabetes have become a serious problem affecting public health and have brought Heavy social and medical economic burden. Based on this, it is of great significance to deve...

Claims

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Application Information

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IPC IPC(8): D04H1/728D01D5/00D01D5/34D01F8/02D01F8/14D01F8/10
CPCD04H1/728D01D5/0007D01D5/0069D01D5/34D01F8/02D01F8/10D01F8/14D10B2509/022
Inventor 陈晓峰孙璐瑶曹晓东原波
Owner 佛山今兰生物科技有限公司
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