Double-dominant marker for accurately authenticating S/N cytoplasm genotype of onions and application of double dominant marker

Abstract

The invention discloses a double-dominant marker for accurately authenticating an S / N cytoplasm genotype of onions and application of the double-dominant marker. The length of a specific fragment of an S-form cytoplasm in the double-dominant marker is 232bp, and the length of a specific fragment of an N-form cytoplasm is 196bp. The invention further discloses SCAR marking primers SEQ ID NO.5 and SEQ ID NO.6. A prepared SCAR molecular marker is utilized for rapidly and accurately judging cytoplasm types of onion materials, so that the misclassification of cytoplasm genotypes caused due to experimental operate miss and invalid PCR amplification can be avoided; and furthermore, DNA can be extracted from mixed group samples when a maintainer line is selected in a large group and can be used for primarily screening whether a maintainer line single plant exists, so that the selection efficiency is greatly improved, the time and the cost are saved, and the double-dominant marker has importantsignificances to the matching of a breeding sterile line and a maintainer line of the onions and the breeding of hybrid varieties of the onions.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, relates to a molecular marker for onion breeding and its application, in particular to a double-dominant molecular marker for identifying the S / N cytoplasmic genotype of onion and its application, which can be applied to molecular marker-assisted breeding Accurate selection of onion male sterile lines and matching maintainer lines. Background technique [0002] Onion (Allium cepa.L), also known as bulb onion, round onion, jade onion, onion, Dutch onion, Piyazi, etc., is a biennial diploid plant belonging to the Liliaceae Allium genus. According to records, it has a cultivation history of more than 5,000 years , onion was introduced into my country in modern times, and it has only been cultivated in my country for more than 100 years. However, due to its strong adaptability and resistance to storage and transportation, although the history of onion planting in my country is short, its develo...

AI Technical Summary

Problems solved by technology

Although this marker can effectively avoid misclassification of cytoplasmic types due to experimental manipulation errors and ineffective PCR amplification, due to the lack of stable N-type cytoplasmic specific markers, it is impossible to select maintainer lines from large populations. Extract DNA from mixed population samples for preliminary screening of maintainer individual plants, which can only be verified by individual plants, which affects the selection efficiency

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