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Fructificatio amaurodermatis rudae hypha fermentation broth, preparation method thereof and cosmetic with oxidation-resisting and whitening effects

A technology of fermentation broth and mycelium, applied in the field of fermentation of black lucid ganoderma lucidum, can solve the problems of low tyrosinase activity inhibition rate, difference in composition and content of active ingredients, low content of polysaccharides and triterpenes, etc. Amidase activity inhibition rate and antioxidant capacity, inhibition of melanin formation, effects of high polysaccharide and triterpenoid content

Active Publication Date: 2018-04-06
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a kind of preparation method of fermented liquid of black sesame mycelium, to solve the problem that the production cycle of black sesame cultivation method in the prior art is longer, needs to adopt fruiting bodies, the cost is higher, and black sesame mycelium protoplasm tyrosine The inhibition rate of acidase activity is not high, the antioxidant function is not ideal, the content of polysaccharides and triterpenes is not high, it is difficult to scale production, and there are large differences in the composition and content of active ingredients between different batches.

Method used

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  • Fructificatio amaurodermatis rudae hypha fermentation broth, preparation method thereof and cosmetic with oxidation-resisting and whitening effects
  • Fructificatio amaurodermatis rudae hypha fermentation broth, preparation method thereof and cosmetic with oxidation-resisting and whitening effects
  • Fructificatio amaurodermatis rudae hypha fermentation broth, preparation method thereof and cosmetic with oxidation-resisting and whitening effects

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preparation example Construction

[0015] The present invention relates to a kind of preparation method of black sesame mycelia fermentation liquid, comprising:

[0016] The black sesame strain is cultured in inclined test tubes to obtain mycelium blocks, and the mycelium blocks are inoculated on plate medium for mycelial culture, then transferred to malt medium, and cultured in liquid fermentation at 25-29°C for 4-7 days.

[0017] Preferably, if the purpose of preparation is to obtain the fermented liquid of black sesame mycelium with the most polysaccharide content, then the liquid fermentation is cultivated for 4 days; if the purpose of the preparation is to obtain the fermented liquid of black sesame mycelium with the highest content of triterpenes, then the liquid fermentation is cultivated for 6 days; If the preparation purpose is to obtain the black sesame mycelia fermentation liquid with the best antioxidant effect, the liquid fermentation culture is 4 days; if the preparation purpose is to obtain the bl...

Embodiment

[0050] 1. Mycelium fermentation broth method

[0051] (1), preparation of culture medium and solution

[0052] Plate medium: MEA (Malt Extract Ager): malt extract 30g, soybean peptone 3g, agar malt extract 3w / v%, soybean peptone 0.3w / v%, agar 1.5w / v%, h 2 O 1L to constant volume, pH 5.6, sterilized at 121°C for 15 minutes.

[0053] Malt medium: malt extract powder 2w / v%, glucose 2w / v%, peptone 0.1w / v%, yeast extract 0.1w / v%, H 2 O 1L to constant volume, sterilized at 115°C for 20min.

[0054] CYM medium: glucose 20g, peptone 2g, yeast powder 2g, MgSO 4 ﹒ 7H 2 O0.5g, K 2 HPO 4 1g, KH 2 PO 4 0.46g, H 2 O 1L.

[0055] (2), liquid fermentation culture method of Heizhi bacterial strain

[0056] After the Heizhi strain was cultured in an inclined test tube, take one piece of 1cm 2 The mycelium block was inoculated into the plate medium, and cultured at 30°C for 7 days. Then take 1.5cm from the plate medium 2 Inoculate into malt culture medium (300ml Erlenmeyer flask...

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Abstract

The invention relates to the technical field of fermentation of Fructificatio amaurodermatis rudae, in particular to Fructificatio amaurodermatis rudae hypha fermentation broth, a preparation method thereof and cosmetic with oxidation-resisting and whitening effects. The method comprises the following steps: Fructificatio amaurodermatis rudae strains are cultured by slant tubes, hypha blocks are obtained, a plate medium is inoculated with the hypha blocks for hypha culture, and then the hypha blocks are transferred to a malt medium and subjected to liquid fermentation at 25-29 DEG C for 4-7 days. The preparation method of the Fructificatio amaurodermatis rudae hypha fermentation broth is simple to operate, the content of polysaccharides and triterpene in the prepared Fructificatio amaurodermatis rudae hypha fermentation broth is high, and the Fructificatio amaurodermatis rudae hypha fermentation broth has high physiological activity and good health care effect; besides, the Fructificatio amaurodermatis rudae hypha fermentation broth has high inhibition rate on tyrosinase activity and oxidation-resisting capacity, so that melanin formation can be inhibited effectively, and senescence can be delayed.

Description

technical field [0001] The invention relates to the technical field of fermentation of black lucidum ganoderma, in particular to a fermented liquid of black lucidum mycelium and a preparation method thereof, and a cosmetic with anti-oxidation and whitening effects. Background technique [0002] Black lucidum, also known as "Chinese Ganoderma lucidum", is sweet in taste and flat in nature. It mainly contains ergosterol, organic acids, glucosamine, polysaccharides, resins, mannitol, polysaccharide alcohols, fatty acids, alkaloids, lactones, coumarin, water-soluble proteins and various enzymes. Black sesame has a wide range of applications, and it can be taken regardless of the weakness of the heart, lungs, liver, spleen, and kidneys. Scientific research shows that the pharmacological components of Ganoderma lucidum are very rich, and the active ingredients can be divided into ten categories, including Ganoderma lucidum polysaccharides, Ganoderma lucidum polypeptides, three an...

Claims

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Application Information

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IPC IPC(8): C12N1/14A61K8/9728A61Q19/02A61Q19/08C12R1/645
CPCA61Q19/02A61Q19/08C12N1/14
Inventor 谢纯良彭源德龚文兵冯湘沅朱作华严理胡镇修
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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