Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing water-dispersing molecular imprinting fluorescent nanoparticles based on macromolecule self-assembling

A technology of molecular imprinting and fluorescent nanometers, applied in chemical instruments and methods, alkali metal oxides/hydroxides, inorganic chemistry, etc., can solve problems such as complex preparation process, limited application in detection field, inability to identify target molecules, etc., to achieve The effect of high selectivity, widening the preparation and application environment, and fast adsorption speed

Active Publication Date: 2018-04-06
郎溪品旭科技发展有限公司
View PDF10 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it cannot directly identify target molecules, and the preparation process is relatively complicated, which limits its application in the detection field.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing water-dispersing molecular imprinting fluorescent nanoparticles based on macromolecule self-assembling
  • Method for preparing water-dispersing molecular imprinting fluorescent nanoparticles based on macromolecule self-assembling
  • Method for preparing water-dispersing molecular imprinting fluorescent nanoparticles based on macromolecule self-assembling

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The first step: Add monomer acrylic acid (0.3603g, 5mmol), vinylcarbazole (0.9663g, 5mmol), azobisisobutylcyanide (0.0328g, 2%mt%) into a 50ml round bottom flask, add solvent N,N-Dimethylformamide (DMF) 15mL, through N 2 After removing the oxygen in the solution for 20min, under stirring, the temperature was kept at 80°C and reacted in an oil bath for 24h. The monomer ethylene glycol methacrylate (0.711g, 5mmol), triphenylphosphine (0.0262g, 0.1mmol) and hydroquinone (0.0028g, 0.025mmol) were dissolved in 5mL of DMF, and were added dropwise to the above polymer solution through a constant pressure dropping funnel. Under stirring, the temperature was maintained at 95°C in oil React in the bath for 12 hours, precipitate with petroleum ether three times, and dry in a vacuum oven at 40°C overnight to obtain a photosensitive fluorescent polymer;

[0023] Step 2: Dissolve 10 mg of the above polymer in DMF, a good solvent, and add 10 μL of paracetamol in DMF (2 mg mL -1 ), ...

Embodiment 2

[0026] The first step: Add monomer acrylic acid (0.7206g, 10mmol), vinyl carbazole (0.9663g, 5mmol), azobisisobutylcyanide (0.0328g, 2%mt%) into a 50ml round bottom flask, add solvent N,N-Dimethylformamide (DMF) 15mL, through N 2 After removing the oxygen in the solution for 20min, under stirring, the temperature was kept at 90°C and reacted in an oil bath for 24h. The monomer ethylene glycol methacrylate (1.422g, 10mmol), triphenylphosphine (0.0262g, 0.1mmol) and hydroquinone (0.0028g, 0.025mmol) were dissolved in 5mL of DMF, and were added dropwise to the above polymer solution through a constant pressure dropping funnel, and the temperature was kept at 95°C under stirring. React in the bath for 12 hours, precipitate with petroleum ether three times, and dry in a vacuum oven at 40°C overnight to obtain a photosensitive fluorescent polymer;

[0027] Step 2: Dissolve 10 mg of the above polymer in DMF, a good solvent, and add 10 μL of paracetamol in DMF (2 mg mL -1 ), 5 μL cro...

Embodiment 3

[0030] The first step: monomer methacrylic acid (0.4403g, 5mmol), vinyl carbazole (1.9326g, 10mmol), azobisisobutylcyanide (0.0328g, 2%mt%) were added in a 50ml round bottom flask, Add solvent N,N-dimethylformamide (DMF) 15mL, pass N 2 After removing the oxygen in the solution for 20min, under stirring, the temperature was kept at 80°C and reacted in an oil bath for 24h. The monomer ethylene glycol methacrylate (0.711g, 5mmol), triphenylphosphine (0.0262g, 0.1mmol) and hydroquinone (0.0028g, 0.025mmol) were dissolved in 5mL DMF, and were added dropwise to the above polymer solution through a constant pressure dropping funnel. React in the bath for 12 hours, precipitate with petroleum ether three times, and dry in a vacuum oven at 40°C overnight to obtain a photosensitive fluorescent polymer;

[0031] Step 2: Dissolve 10 mg of the above polymer in DMF, a good solvent, and add 10 μL of paracetamol in DMF (2 mg mL -1 ), 10 μL crosslinker divinylbenzene and 20 μL photoinitiator...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing water-dispersing molecular imprinting fluorescent nanoparticles based on macromolecule self-assembling, relating to the scientific and technical fields of material science, self-assembling, fluorescence analysis and detection and the like. According to the method, hydrophobic fluorescent monomers and hydrophilic monomers are copolymerized and are modified by virtue of monomers containing double bonds so as to generate a photosensitive fluorescent amphophilic polymer; the molecular imprinting fluorescent nanoparticles are prepared through macromolecule self-assembling and photo-crosslinking, and the specific recognition and detection of a molecular imprinting material to template molecules in a water phase are realized; and meanwhile, the material is endowed with a relatively large specific surface area by virtue of a nano-structure, so that the material has more active binding sites and is relatively high in mass transfer rate and bindingkinetics. By combining high sensitivity of fluorescence detection, the selectivity of a molecular imprinting technique and macromolecule self-assembling, the obtained molecular imprinting fluorescentnanoparticles can be applied to high-selectivity and high-sensitivity rapid detection of trace micromolecules or biomacromolecules in an actual sample and particularly have relatively large utilization potentials in the fields of food safety detection, environmental and water-pollution detection, clinical analysis and the like.

Description

technical field [0001] The invention relates to the scientific and technological fields of polymer material science, self-assembly and fluorescence analysis and detection, and in particular to a method for preparing water-dispersible molecularly imprinted fluorescent nanoparticles based on macromolecular self-assembly Background technique [0002] Molecularly imprinted polymers have cavities whose shapes match those of substrate molecules, and functional groups with specific arrangements that can recognize substrate molecules. Compared with conventional and conventional separation and analysis media, the outstanding feature of molecularly imprinted polymers based on molecular recognition is their high selectivity for the separated substance or analyte. Most of the molecular recognition sites on the imprinted polymers prepared by traditional methods are embedded in polymers with high cross-linking density, which leads to molecularly imprinted materials with high molecular rec...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): B01J20/26B01J20/28B01J20/30
CPCB01J20/268B01J20/28004B01J20/28011
Inventor 罗静黄婧邰名扬朱叶刘晓亚
Owner 郎溪品旭科技发展有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com