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Method for counting different grades of red imported fire ants and application

A counting method and technology of RIFA, applied in sampling devices, animal husbandry, etc., can solve the problem of not reflecting the occurrence of ant nests, and achieve the effect of high counting accuracy

Active Publication Date: 2018-04-06
INST OF AGRI ENVIRONMENT & RESOURCES YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method can only reflect the growth and decline dynamics of worker ants, and cannot reflect the occurrence of other levels of ant nests throughout the year. In order to more accurately grasp the occurrence dynamics of different levels of ant colonies and find the vulnerable period of RIFA in ant nests, it is necessary to The counting of different grades of RIFA nests provides a certain scientific basis for the prevention and control work

Method used

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  • Method for counting different grades of red imported fire ants and application
  • Method for counting different grades of red imported fire ants and application
  • Method for counting different grades of red imported fire ants and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Specific steps are as follows:

[0037] 1. Sampling tools: talcum powder, scissors, steel tape, GPS, plastic bucket, notebook, tape paper, shovel, etc.

[0038] 2. Sampling: Select an ant nest that has not been damaged by interference, first smear the inner wall of the plastic bucket and the handle of the shovel with talcum powder to prevent the ants from biting people when they attack, and then use scissors to cut the weeds on the ant nest and Make other plants clean, and then measure the length, width, and height of the nest. According to the cross shape, take a quarter of the soil of the ant nest on the ground longitudinally. Quickly pour the sample into a plastic bucket with a shovel, seal it with tape, attach a label, and mark the relevant information of the collection site at the same time, and bring the sample back to the room for processing.

[0039] 3. Sample pretreatment: There are a lot of soil, weeds, gravel, grass seeds, red fire ants, etc. in the samples taken ...

Embodiment 1

[0044] Implementation 1: Take the 30,000 soldier ants counted in the control 1 as a sample, put 30,000 soldier ants in a test tube, just soak in absolute ethanol, and the ethanol concave surface just keeps the level of the soldier ants. The ants are evenly distributed in the grid ice box. The grid ice box is actually a grid model. The capacity of each square is the same. When the ants are divided, the tightness and height of each square should be kept as consistent as possible. Pack a total of 10 squares. Randomly select the number of soldiers in 3 squares for statistics. Record the time required for each square to count soldiers. This method is the volume method, where the number of soldiers ants = average number of soldiers ants in 3 squares * total number of squares.

Embodiment 2

[0045] Implementation 2: Take the 30,000 soldier ants counted in the control 1 as the sample, and place the sample on a 100-mesh nylon net. The purpose is to filter the absolute ethanol and dry the sample. When the ethanol just evaporates, weigh the sample with an electronic balance The total mass, then put the sample in the circular tray, shake the sample 10 times evenly to distribute the samples evenly, take 3 samples at random and put them in a 3ml plastic container and weigh the sample mass separately, and count each sample Count and record the time required for each sample. This method is a weighing method. Number of soldier ants = total weight of soldier ants / average mass of sample * average number of samples.

[0046] Count 30,000 soldiers ants in the sample by visual inspection, volumetric method, and weighing method, and analyze the effect of different counting methods, mainly depending on the accuracy of the counting and the time required for statistical data. The relat...

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Abstract

The invention provides a method for counting different grades of red imported fire ants. The method comprises the steps that the size of an ant nest is measured, and the ant nest is sampled; after anant nest sample is preprocessed and soil is removed, the sample is soaked with absolute ethyl alcohol; then, impurities are separated and removed to obtain a clean and impurity-free red imported fireant sample, and the sample is soaked with absolute ethyl alcohol; filtration is performed to remove the ethyl alcohol, the red imported fire ant sample is air-dried and screened by using a screen, andlarger-sized breeding ants are selected out for separate counting; the total mass of the red imported fire ants on the screen and the red imported fire ants under the screen are respectively weighed,then a small amount of sample is taken to weigh sample mass, the numbers of different grades of red imported fire ants in the sample are counted, and the numbers of different grades of red imported fire ants in the ant nest = the total mass of the red imported fire ants on the screen or under the screen / the mass of the sampled red imported fire ants on the screen or under the screen * the numbersof different grades of red imported fire ants in the sample, and the densities of different grades of red imported fire ants is calculated. By adopting the method, the structures of red imported fireants and the outbreak regularity of different grades of red imported fire ants can be known in detail. The method is high in accuracy, different grades and densities of ants can be applied to the outbreak regularity of different grades of red imported fire ants, and a scientific basis is provided for prevention and control of local red imported fire ants.

Description

Technical field [0001] The invention relates to a method for counting different grades of red imported fire ant nests and its application, in particular to the processing, counting and application of different grades of red imported fire ant samples, and belongs to the field of invasive pest control research. Background technique [0002] The red fire ant Solenopsis invicta Buren belongs to Hymenoptera, Formicidae, Mymicinae, and solenopsis. It is a dangerous pest native to Paraná Valley in South America and is listed as the most dangerous in the world. One of the 100 species of invasive pests in the invasion area has a significant impact on agricultural production, ecosystems and human health in the invasion area. After the RIFA invaded Taiwan, my country in 2004, it occurred in Wuchuan, Guangzhou, Shenzhen, Dongguan, Huizhou, Guangxi Beihai, Nanning, Zhangjiajie, Hunan, and Longyan, Fujian. It was discovered in Yuanmou County, Chuxiong Prefecture, Yunnan in October 2013, and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033G01N1/04
CPCA01K67/033G01N1/04
Inventor 张红梅陈福寿王燕谌爱东陈宗麒徐兴才杨艳鲜
Owner INST OF AGRI ENVIRONMENT & RESOURCES YUNNAN ACAD OF AGRI SCI
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